Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,590,024 A→C 5.6% E182A (GAA→GCA)  nicP‑I → porin‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,590,0240AC 5.6% 265.4 / 3.3 84E182A (GAA→GCA) nicP‑Iporin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (41/38);  new base C (3/2);  total (44/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAACCGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAG  >  NC_002947/3589883‑3590166
                                                                                                                                             |                                                                                                                                              
tGCAACCGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTTGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGc                                                                                                                                         >  7:269156/1‑149 (MQ=255)
tGCAACCGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGc                                                                                                                                         >  6:328163/1‑149 (MQ=255)
 gCAACCGAACCTGCCGGTGCTCACATTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGATTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCAGGGCACTTGAAAAGCACCCACCTGAGTAACGAAGGTGGc                                                                                                                                         >  5:8683/1‑148 (MQ=255)
  cAACCGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGa                                                                                                                                       >  3:247297/1‑149 (MQ=255)
   aaCCGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGAt                                                                                                                                      >  5:44082/1‑149 (MQ=255)
     ccGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATgg                                                                                                                                    >  3:256523/1‑149 (MQ=255)
      cGAACCTGCCGTTGCAGAGCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACTGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGc                                                                                                                                   <  2:244959/149‑1 (MQ=255)
      cGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAg                                                                                                                                              <  4:101758/138‑1 (MQ=255)
      cGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAg                                                                                                                                              >  3:101758/1‑138 (MQ=255)
                  tGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAAC                                                                                                                          <  6:18981‑M1/146‑1 (MQ=255)
                  tGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAAC                                                                                                                          >  5:18981‑M1/1‑146 (MQ=255)
                      gACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGc                                                                                                                   >  8:198958/1‑149 (MQ=255)
                      gACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGAGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGc                                                                                                                   >  8:193704/1‑149 (MQ=255)
                       aCCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATg                                                                                                                    <  5:323481/147‑1 (MQ=255)
                          ttCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATACACGGGCTGACTATGCAGGCCGGGCAATTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCgc                                                                                                               >  5:231364/1‑148 (MQ=255)
                             aGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCa                                                                                                            >  8:173000/1‑149 (MQ=255)
                                  tatTCGCCTGTTGCCACCCACCTATCAGTGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTTCGTTACGCAg                                                                                                                                              <  8:180725/110‑1 (MQ=255)
                                  tatTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTTCGTTACGCAg                                                                                                                                              >  7:180725/1‑110 (MQ=255)
                                  tatTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGc                                                                                                       >  2:206633/1‑149 (MQ=255)
                                  tatTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGc                                                                                                       >  3:123204/1‑149 (MQ=255)
                                     tCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGa                                                                                                    >  2:133190/1‑149 (MQ=255)
                                     tCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGa                                                                                                    >  5:190943/1‑149 (MQ=255)
                                       gCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTTGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGAt                                                                                                   <  8:269156/148‑1 (MQ=255)
                                       gCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTTACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGAt                                                                                                   <  1:85473/148‑1 (MQ=255)
                                       gCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATg                                                                                                  >  5:65711/1‑149 (MQ=255)
                                                 acccaccTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGcc                                                                                         >  1:151242/1‑148 (MQ=255)
                                                   ccaccTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAgg                                                                                                                                             <  7:326954/94‑1 (MQ=255)
                                                   ccaccTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAgg                                                                                                                                             >  8:326954/1‑94 (MQ=255)
                                                     accTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAg                                                                                    <  8:124432/149‑1 (MQ=255)
                                                         aTCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGAc                                                                                <  4:3861‑M2/149‑2 (MQ=255)
                                                               gCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGtcgctttcggcctgtcgcatcggcacatggccgagcatcgcgttc                                                                          <  1:216438‑M1/149‑46 (MQ=255)
                                                                     gTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTAt                                                                    <  7:198958/149‑1 (MQ=255)
                                                                     gTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTAt                                                                    <  7:193704/149‑1 (MQ=255)
                                                                         gAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGc                                                                                          <  3:164181/123‑1 (MQ=255)
                                                                         gAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGc                                                                                          >  4:164181/1‑123 (MQ=255)
                                                                         gAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTAGgtgg                                                                >  7:2827/1‑149 (MQ=255)
                                                                               gtcgGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGtcgctttcggcctgtcgcatcggcacatggccgagcatcgcgttcat                                                                        <  5:197743‑M1/135‑48 (MQ=255)
                                                                               gtcgGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGtcgctttcggcctgtcgcatcggcacatggccgagcatcgcgttcat                                                                        >  6:197743‑M1/1‑88 (MQ=255)
                                                                               gtcgGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATgtgggtgg                                                            <  5:180628/147‑1 (MQ=255)
                                                                                tcgGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGa                                                         >  4:256025/1‑149 (MQ=255)
                                                                                  ggAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGAc                                                        >  6:222443/1‑148 (MQ=255)
                                                                                  ggAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGAc                                                        >  8:176078/1‑148 (MQ=255)
                                                                                    tattCGAGGGGCTGTCTATTCAGGGCGGGCACTTGAAAATCACCCACCTGCTTAACGAAGGTGGAGATGGCAAGATGATCGCGATGCTCGGCCTTGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTAt                                                     <  6:231364/146‑1 (MQ=255)
                                                                                    aaaTCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTAt                                                     <  6:190943/149‑1 (MQ=255)
                                                                                       tCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCg                                                  <  7:173000/149‑1 (MQ=255)
                                                                                         gACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTAt                                                     <  7:329681/144‑1 (MQ=255)
                                                                                         gACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTAt                                                     >  8:329681/1‑144 (MQ=255)
                                                                                         gACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGtt                                                <  1:206633/149‑1 (MQ=255)
                                                                                         gACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGt                                                 <  4:247297/148‑1 (MQ=255)
                                                                                            gggCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCaa                                             <  5:222443/149‑1 (MQ=255)
                                                                                              gCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATg                                           >  7:201878/1‑149 (MQ=255)
                                                                                               cTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGt                                                                                                                                            <  6:163585/51‑1 (MQ=255)
                                                                                               cTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGt                                                                                                                                            >  5:163585/1‑51 (MQ=255)
                                                                                                 gACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGcc                                         >  6:82284/1‑148 (MQ=255)
                                                                                                  aCTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGccaa                                       >  8:193355/1‑149 (MQ=255)
                                                                                                  aCTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGcca                                        <  1:133190/148‑1 (MQ=255)
                                                                                                   cTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGccaa                                       <  3:3861/148‑1 (MQ=255)
                                                                                                    tATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTAtgt                                                                  <  8:178112/120‑1 (MQ=255)
                                                                                                    tATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTAtgt                                                                  >  7:178112/1‑120 (MQ=255)
                                                                                                    tATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGccaac                                      >  5:329502/1‑148 (MQ=255)
                                                                                                      tGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGccaacca                                    >  6:218912/1‑148 (MQ=255)
                                                                                                        cAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAag                                 >  5:16997/1‑149 (MQ=255)
                                                                                                        cAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAag                                 >  5:11843/1‑149 (MQ=255)
                                                                                                        cAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAag                                 <  4:256523/149‑1 (MQ=255)
                                                                                                         aGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAagc                                <  5:218912/149‑1 (MQ=255)
                                                                                                                gCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGt                           >  4:20418/1‑147 (MQ=255)
                                                                                                                  acatGAAAACCACCCACCTGCGTAACGAAGGTGGCGATGGCACGATGAACTCGATGCTCGGCCATGTGCCGATGCGACACGCCGAAATCGACGCGTTCATCTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGGAGCGTCAgg                       <  8:2827/146‑2 (MQ=255)
                                                                                                                  aCTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGc                       >  7:217238/1‑149 (MQ=255)
                                                                                                                  aCTAGAAAAGCACCCACCTACGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATCTGGGTGGGGACTATGCGTTCAATGCCAACCAAACCAGCGTCAGc                       >  8:121227/1‑149 (MQ=255)
                                                                                                                    ttGAAAAGCACCCACCTGCGTAACGCAGATGGCGATGACAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGCAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCt                     >  8:54205/1‑149 (MQ=255)
                                                                                                                       aaaaGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCttt                   <  7:193355/148‑1 (MQ=255)
                                                                                                                           gCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTAc              >  4:101331/1‑149 (MQ=255)
                                                                                                                              gccaccTTCGTTACGCAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCa                         >  1:43689/2‑135 (MQ=255)
                                                                                                                              gccaccTTCGTTACGCAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCa                         <  2:43689/134‑1 (MQ=255)
                                                                                                                               ccaccTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTc          <  2:151242/149‑1 (MQ=255)
                                                                                                                                   cTGCGTAACGAAGGTGGCGATGGCAAGATGNACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCt      >  1:28470/1‑149 (MQ=255)
                                                                                                                                      cGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGTTCAGCTgga   <  6:11843/149‑1 (MQ=255)
                                                                                                                                      cGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTgga   <  6:16997/149‑1 (MQ=255)
                                                                                                                                      cGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTgga   <  3:20418/149‑1 (MQ=255)
                                                                                                                                      cGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTgga   <  4:123204/149‑1 (MQ=255)
                                                                                                                                       gTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGTGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTgga   <  7:121227/148‑1 (MQ=255)
                                                                                                                                       gTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTggag  <  6:329502/149‑1 (MQ=255)
                                                                                                                                        tAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTgg                 <  1:45901/133‑1 (MQ=255)
                                                                                                                                        tAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTgg                 >  2:45901/1‑133 (MQ=255)
                                                                                                                                             |                                                                                                                                              
TGCAACCGAACCTGCCGGTGCTGACCTTCAGTGATATTCGCCTGTTGCCACCCACCTATCAGGGCGTGAGTTTGAGCTCGTCGGAAATCGACGGGCTGACTATGCAGGCCGGGCACTTGAAAAGCACCCACCTGCGTAACGAAGGTGGCGATGGCAAGATGAACGCGATGCTCGGCCATGTGCCGATGCGACAGGCCGAAAGCGACGCGTTCAACTATGTGGGTGGGGACTATGCGTTCAATGCCAACCAAAGCAGCGTCAGCCTTTGGTACGGTCAGCTGGAG  >  NC_002947/3589883‑3590166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑