Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,617,993 C→T 7.0% Q340* (CAG→TAG)  PP_3188 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,617,9930CT 7.0% 164.2 / 2.1 57Q340* (CAG→TAG) PP_3188hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/28);  new base T (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

GGTATTGTCGACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGAAGCCCACGGCAAGGA  >  NC_002947/3617849‑3618141
                                                                                                                                                |                                                                                                                                                    
ggTATTGTCGACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCg                                                                                                                                                  >  4:219738/1‑149 (MQ=255)
  tATTGTCGACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGTTCATTGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTc                                                                                                                                                <  7:226585/149‑1 (MQ=255)
   aTTGTCGACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCt                                                                                                                                               >  4:82651/1‑149 (MQ=255)
      gTCGACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTgg                                                                                                                                             <  3:219738/148‑1 (MQ=255)
         gACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCATACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATAGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCt                                                                                                                                         >  1:267277/1‑149 (MQ=255)
            cGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCa                                                                                                                                      >  8:305673/1‑149 (MQ=255)
              ttCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCAcc                                                                                                                                    >  1:1025/1‑149 (MQ=255)
                  cTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGCCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCaa                                                                                                                                >  5:325368/1‑149 (MQ=255)
                     gTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGACCCTTGACGCAAACACGCCCATGCTTTCGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGTCCGCACACCGTCTGGGGATGCACCACAacac                                                                                                                             >  3:55536/1‑149 (MQ=255)
                        gatgatTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCACCGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGat                                                                                                                          >  7:330705/1‑149 (MQ=255)
                         atgatTTCGTCAGCAGCACCCTCGGCCCGCTGCTCGCCCGTGGACGCAAACACGCCCATCCTTTGGTGCATACCCTCGTGGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCAGCGCAACACGatc                                                                                                                         <  4:247862/149‑1 (MQ=255)
                          tgatTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGatca                                                                                                                        <  3:82651/149‑1 (MQ=255)
                                 gTCAGCCGCACCCTCGGCCCGCTGTTCGCCCCTGGACGCAAACACGGCCATGCTTTGGTGCATACGCTCGACGCCTTGCTCATGGTATCTGGCAACGGGCGCAAGGCCGCACAGCGTCTGGGGCTGCACCg                                                                                                                                   <  7:23103/131‑1 (MQ=255)
                                 gTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATcagc                                                                                                                  <  5:85458/148‑1 (MQ=255)
                                 gTCAGCCGCACCCTCGCCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGACTTGCTCATGGAAAATGGCAACGGGATCAAGGCCGCACAGCGTCTGGGGCTGCACCg                                                                                                                                   >  8:23103/1‑131 (MQ=255)
                                       cGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGTAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATcagcgcatcca                                                                                                           <  3:234352/149‑1 (MQ=255)
                                       cGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATcagcgcatc                                                                                                             >  6:275084/1‑147 (MQ=255)
                                         cACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGAAACACGATCAATcagcgcatccagc                                                                                                         >  3:85791/1‑149 (MQ=255)
                                            cctatgCCCTCTGATAGCCCCTGGACGCAAACACGCCCATGCTTTGGTTCATACCCTAGACGCCTTTCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCTTCTGGGGCTGCACCGCAACAAGAACAATcagcgcatccagcgca                                                                                                      <  4:85791/144‑1 (MQ=255)
                                            ccTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATcagcgcatccagcgc                                                                                                       >  4:107214/1‑148 (MQ=255)
                                            ccTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATcagcgcatccagcgc                                                                                                       <  3:107214/148‑1 (MQ=255)
                                                    cGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGaacaa                                                                                              >  5:292632/1‑149 (MQ=255)
                                                    cGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGaacaa                                                                                              <  2:1025/149‑1 (MQ=255)
                                                      cTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGaacaac                                                                                             <  8:285834/148‑1 (MQ=255)
                                                      cTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGaacaac                                                                                             >  7:285834/1‑148 (MQ=255)
                                                         aTCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAg                                                                                         <  3:5788/149‑1 (MQ=255)
                                                             ccccTGGACGCATACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATAGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCggg                                                                                     <  2:267277/149‑1 (MQ=255)
                                                               ccTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCggg                                                                                     >  4:222857/1‑147 (MQ=255)
                                                                         aaCACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGatcaat                                                                                                                      >  1:1974/1‑104 (MQ=255)
                                                                         aaCACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGatcaat                                                                                                                      <  2:1974/104‑1 (MQ=255)
                                                                            acGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCATAGCGTCTgggg                                                                                                                                           <  5:293252/80‑1 (MQ=255)
                                                                            acGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCATAGCGTCTgggg                                                                                                                                           >  6:293252/1‑80 (MQ=255)
                                                                                      tttGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGat                                                                                                                          <  7:165151/87‑1 (MQ=255)
                                                                                      tttGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGat                                                                                                                          >  8:165151/1‑87 (MQ=255)
                                                                                        tGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTc                                                            <  5:191284/147‑1 (MQ=255)
                                                                                          gTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGGATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCa                                                         <  6:292632/148‑1 (MQ=255)
                                                                                          gTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCAt                                                        <  5:275084/149‑1 (MQ=255)
                                                                                                  ccTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCATAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCtt                                                >  2:10992/1‑149 (MQ=255)
                                                                                                      gACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCg                                              <  3:222857/147‑1 (MQ=255)
                                                                                                        cGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCg                                          <  7:305673/149‑1 (MQ=255)
                                                                                                                  aTGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCgg                                             >  7:69431/1‑136 (MQ=255)
                                                                                                                  aTGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCgg                                             <  8:69431/136‑1 (MQ=255)
                                                                                                                  aTGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTgg                                 >  2:265858/1‑148 (MQ=255)
                                                                                                                       aaaTGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGgc                           <  1:265858/149‑1 (MQ=255)
                                                                                                                            gcatctggCTCAAGGGTGCACAGCGTCTGGGGCTGCACCAGTTCAGGATCTATGAGCGCATCCAGCGCATCGAACATCTGATCGGGCTGTGGATGGACGACCCGCTTTTTCGAATGAATGCTTCGGTCGCCATTCTGGTATGGCGGAt                       <  4:55536/142‑1 (MQ=255)
                                                                                                                            gCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCAt                       >  1:335750/1‑148 (MQ=255)
                                                                                                                              aaCGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGaacaac                                                                                             <  5:327694/76‑1 (MQ=255)
                                                                                                                              aaCGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGaacaac                                                                                             >  6:327694/1‑76 (MQ=255)
                                                                                                                               aCGGGCTCAAGGCCGCATAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGc                   <  1:10992/149‑1 (MQ=255)
                                                                                                                                  ggCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGaa                <  6:325368/149‑1 (MQ=255)
                                                                                                                                   gCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCtt                                                                <  6:306876/100‑1 (MQ=255)
                                                                                                                                   gCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCtt                                                                >  5:306876/1‑100 (MQ=255)
                                                                                                                                     tCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGgcgc                         <  6:176947/137‑1 (MQ=255)
                                                                                                                                     tCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGgcgc                         >  5:176947/1‑137 (MQ=255)
                                                                                                                                       aaGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGAGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGAAGCCCa           >  5:251468/1‑149 (MQ=255)
                                                                                                                                         ggCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGAAGCCCACg         <  2:335750/149‑1 (MQ=255)
                                                                                                                                            cGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGAAGCCCACGGc       >  8:36043/1‑148 (MQ=255)
                                                                                                                                                caGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGAAGCCCACGGCAAGGa  <  7:36043/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGTATTGTCGACCGTTCGCTGGTCGATGATTTCGTCAGCCGCACCCTCGGCCCGCTGATCGCCCCTGGACGCAAACACGCCCATGCTTTGGTGCATACCCTCGACGCCTTGCTCATGGAAAATGGCAACGGGCTCAAGGCCGCACAGCGTCTGGGGCTGCACCGCAACACGATCAATCAGCGCATCCAGCGCATCGAACAACTGAGCGGGCAGTCGCTGGACGACCCGCTTTTTCGCATGAATGCTTCGGTCGCCATGCTGGTATGGCGCATGAGCGAAGCCCACGGCAAGGA  >  NC_002947/3617849‑3618141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑