Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,624,753	G→A	6.5%	S245S (AGC→AGT)	PP_3195 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,624,753	0	G	A	6.5%	183.0 / 2.2	63	S245S (AGC→AGT)	PP_3195	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base A (2/2);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

CCCACACCACGCCGCAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTT  >  NC_002947/3624617‑3624900                                                                                                                                         |                                                                                                                                                    cccACACCACGCCGCAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAgtgt                                                                                                                                         >  2:204902/1‑149 (MQ=255)      accacGCCGCAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGAATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCt                                                                                                                                    >  1:148045/1‑149 (MQ=255)         acGCCGCAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGt                                                                                                                                  >  8:154627/1‑148 (MQ=255)           tccgcAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCAt                                                                                                                               <  8:304755/148‑1 (MQ=255)             cgcAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATgg                                                                                                                             >  1:53018/1‑149 (MQ=255)              gcaagcaaAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGc                                                                                                                            <  1:204902/149‑1 (MQ=255)                aagcaaAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGt                                                                                                                          >  6:322221/1‑149 (MQ=255)                aagcaaAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGt                                                                                                                          >  2:118339/1‑149 (MQ=255)                   caaAGCGCGTATCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGcc                                                                                                                       <  1:123779/149‑1 (MQ=255)                   caaAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCAAGTGTTCGCTGTCATGGCGTGcc                                                                                                                       >  3:181061/1‑149 (MQ=255)                     aaGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGt                                                                                                                     >  8:127998/1‑149 (MQ=255)                      aGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTc                                                                                                                    <  1:240106/149‑1 (MQ=255)                         gcgGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCAATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATg                                                                                                                 <  7:197435/149‑1 (MQ=255)                               cGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTg                                                                                                            <  2:268554/148‑1 (MQ=255)                                gAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGa                                                                                                          <  7:314082/149‑1 (MQ=255)                                  aCGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAgg                                                                                                        <  1:118339/149‑1 (MQ=255)                                      cACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGGCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGaa                                                                                                    <  5:339994/149‑1 (MQ=255)                                      cACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGc                                                                                                                            >  6:131881/1‑125 (MQ=255)                                      cACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGc                                                                                                                            <  5:131881/125‑1 (MQ=255)                                      cACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCg                                                                                                      >  8:101495/1‑147 (MQ=255)                                            gcgcgGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGACTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCtt                                                                                              >  5:12413/1‑149 (MQ=255)                                            gcgcgGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGACTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCtt                                                                                              >  1:217949/1‑149 (MQ=255)                                             cgcgGACAGTAGTAGCCGCCCAGGGAAACGCCTTACAGGCCGATGCGCCTGGCATCGCCATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATTCCGTGGCGGCGCAGCGCTTc                                                                                             >  5:2126/1‑149 (MQ=255)                                               cgGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGt                                                                                                             >  2:305594/1‑131 (MQ=255)                                               cgGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGc                                                                                           <  7:127998/149‑1 (MQ=255)                                                  aCAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCgg                                                                                        >  3:119620/1‑149 (MQ=255)                                                        ttagCCGCCCTGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCCGCTAGTCCACTTTGGGGCTTGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCg                                                                                   <  7:227066/147‑1 (MQ=255)                                                        gtagCCGCCCAGGGAAACGCCTTCCAGGCCGATGAGCCTGGCATCGACATCAGCTCGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCAGGTGACgg                                                                                  >  6:78556/1‑149 (MQ=255)                                                           gccgccCAGGGAAACGCATTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGc                                                                                <  7:154627/148‑1 (MQ=255)                                                               cccAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTc                                                                           >  8:77218/1‑149 (MQ=255)                                                                 cAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGa                                                                         >  5:237912/1‑149 (MQ=255)                                                                       aaCGCCTTCCCGGCCGATGCGCCGGGCATCCACATCACCACGGGTTTCCAGCCAGTCCACAATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCTTGGAGGCGAAGCTCTTCGCCGGTGCCGGGCTGGTCGACAATCa                                                                   <  5:78556/149‑1 (MQ=255)                                                                         cGCCTTCCCGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCCGCCAGCCCACTATGCGGCTGCCCCAGTGTTCGCGGTCATGGCGTGCGGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGc                                                                 <  2:347778/149‑1 (MQ=255)                                                                          gCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGt                                                                            >  1:222797/1‑137 (MQ=255)                                                                          gCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGt                                                                            <  2:222797/137‑1 (MQ=255)                                                                             ttCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGcgac                                                              >  5:109081/1‑148 (MQ=255)                                                                               ccAGGCCGAGGCGCCGGGCATCGACATCAGCACGGGTTTCCCGCCAGTCCACTATGCGCCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGcgacga                                                            <  6:237912/148‑1 (MQ=255)                                                                                 aGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGacac                                                         >  1:224523/1‑149 (MQ=255)                                                                                   gCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGCCAATCAGCGACGACAcgc                                                       >  7:15469/1‑149 (MQ=255)                                                                                           gcCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCTAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTg                                               <  4:119620/149‑1 (MQ=255)                                                                                           gcCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTg                                               <  6:109081/149‑1 (MQ=255)                                                                                            cgtgccATCGACATCTCCCGGGGTTTCCCCCCCGCCCACTAGGCGGCGGGCCCTGTGTGCGCGGTCTGGGCGGGCCGTCAGGCGGTGGCGGCTAAGCGCTGCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTgg                                              <  8:15469/144‑1 (MQ=255)                                                                                            ccTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTgg                                              >  6:262439/1‑149 (MQ=255)                                                                                             cTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGgc                                             <  7:77218/149‑1 (MQ=255)                                                                                             cTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCCCTTCGCCGGTGCCggg                                                                                 >  6:9185/1‑113 (MQ=255)                                                                                             cTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGACTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGgc                                             <  2:217949/149‑1 (MQ=255)                                                                                             cTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGACTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGgc                                             <  6:12413/149‑1 (MQ=255)                                                                                                  aTCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGgccagcc                                        <  7:101495/149‑1 (MQ=255)                                                                                                     gACATCTCCACGGCTTTCCAGCCACTCGACTATGCGGCTGGCCCAGTGCTGGCTGTCATGGTGTGCGGTCATGCCGTTGAGGCGATGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCcagg                                     <  4:181061/149‑1 (MQ=255)                                                                                                      gcATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCt                                                                               >  6:289735/2‑106 (MQ=255)                                                                                                      gcATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCt                                                                               <  5:289735/105‑1 (MQ=255)                                                                                                      aCATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCTGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCcagg                                     >  5:112080/1‑148 (MQ=255)                                                                                                          cAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTc                                                                           <  3:260866/106‑1 (MQ=255)                                                                                                          cAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTc                                                                           >  4:260866/1‑106 (MQ=255)                                                                                                            gCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCcaggcaggcag                              <  2:224523/149‑1 (MQ=255)                                                                                                             cACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCcaggcaggcagg                             >  2:117784/1‑149 (MQ=255)                                                                                                               cGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCTGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGcc                           <  6:112080/149‑1 (MQ=255)                                                                                                                       cagccagTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGACGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCcaggcagg                                 <  4:138201/135‑1 (MQ=255)                                                                                                                       cagccagTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCcaggcagg                                 >  3:138201/1‑135 (MQ=255)                                                                                                                        agccagGCCACTATGCGGCGGGCCCAGTGCTCGCTGTCATGGCGGGCCGTCATGCCGTGGAGGCGACGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTa                  <  5:322221/149‑1 (MQ=255)                                                                                                                                  cTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTcc        >  7:161281/1‑149 (MQ=255)                                                                                                                                     tGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCtt      <  2:53018/148‑1 (MQ=255)                                                                                                                                       cGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGt   <  1:117784/149‑1 (MQ=255)                                                                                                                                        ggCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGtt  <  5:184877/149‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                    CCCACACCACGCCGCAAGCAAAGCGCGGTTCGAACGCCACTGCGCGCGGACAGTAGTAGCCGCCCAGGGAAACGCCTTCCAGGCCGATGCGCCTGGCATCGACATCAGCACGGGTTTCCAGCCAGTCCACTATGCGGCTGGCCCAGTGTTCGCTGTCATGGCGTGCCGTCATGCCGTGGAGGCGAAGCGCTTCGCCGGTGCCGGGCTGGTCGACAATCAGCGACGACACGCCTCGCCTGGCCAGCCAGGCAGGCAGGCCGACGCGGTATTTCATTTCCTTGGTT  >  NC_002947/3624617‑3624900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑