Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,668,785 A→G 6.3% D177D (GAT→GAC)  PP_3232 ← acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,668,7850AG 6.3% 180.7 / 2.8 64D177D (GAT→GAC) PP_3232acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base G (2/2);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

TTGCCGTAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGCAGCAGGCGACCCAAGCCAT  >  NC_002947/3668643‑3668933
                                                                                                                                              |                                                                                                                                                     
ttGCCGTAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCggg                                                                                                                                                 >  1:183123/1‑149 (MQ=255)
  gCCGTAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTAATGGGGAGCCTAACGTAAACGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGCGTTCAGATCAGGGAGAGCCGGTGGCTGAGCTGGCTGGGATCATCCGGGt                                                                                                                                                >  8:116311/1‑148 (MQ=255)
     gTAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAgc                                                                                                                                            >  4:347131/1‑149 (MQ=255)
      tAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCGGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCgg                                                                                                                                                  <  8:284990/142‑1 (MQ=255)
      tAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCGGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCgg                                                                                                                                                  >  7:284990/1‑142 (MQ=255)
           tttttCCATCAATTTCTTCGAAGGGCCGTTCATGGGTAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGcggcgg                                                                                                                                       <  6:51351/148‑1 (MQ=255)
                 cATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGaa                                                                                                                                <  2:183123/149‑1 (MQ=255)
                 cATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGa                                                                                                                                 <  7:77850/148‑1 (MQ=255)
                   tCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGTCCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATc                                                                                                                              >  6:133636/1‑149 (MQ=255)
                   tCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCgg                                                                                                                                                  <  3:264587/129‑1 (MQ=255)
                   tCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCgg                                                                                                                                                  >  4:264587/1‑129 (MQ=255)
                   tCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATc                                                                                                                              >  1:284616/1‑149 (MQ=255)
                      aTTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTg                                                                                                                           >  3:139683/1‑149 (MQ=255)
                         tcttcGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTCCGACGGCCCACGGTTCAGATCACGGAGAGCCGCTGGATGACCTGGCTCGGATCATCCCGGTCAGCGGCGGTGGTGAATCCTtccg                                                                                                                        >  3:283739/1‑149 (MQ=255)
                           ttcGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTgccgcc                                                                                                                      >  2:99042/1‑149 (MQ=255)
                           ttcGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTgccgcc                                                                                                                      >  8:81584/1‑149 (MQ=255)
                           ttcGAAGGGCCGCTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTgccgcc                                                                                                                      >  6:344769/1‑149 (MQ=255)
                                    ccGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGc                                                                                                             >  7:14135/1‑149 (MQ=255)
                                             gggAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCAt                                                                                                     <  5:344769/148‑1 (MQ=255)
                                                 gCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCtt                                                                                                <  3:347131/149‑1 (MQ=255)
                                                    tCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCttggt                                                                                             >  4:9785/1‑149 (MQ=255)
                                                      ccGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCttggttg                                                                                           >  5:196703/1‑149 (MQ=255)
                                                      ccGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCttggtt                                                                                            <  6:330982/148‑1 (MQ=255)
                                                         tAATCGGGCCGGCGCGCATTGACCCGTGTGTGCTCCGGCGCAGGGTTCAGATGAGGGTGAGTCGGTGGATGACCTGGGTGTGTTGTTGCGGGTCAGCGGCGGTGGTGAATGCTTCCTGCTTGTCCAGCTCGCGCTTCGCTTGTTTGGcc                                                                                        <  4:283739/149‑1 (MQ=255)
                                                                    ccgcGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCACTTGGTTGGCCGCTGCGTCGAc                                                                             <  2:234623/149‑1 (MQ=255)
                                                                        gcATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGaa                                                                         <  3:108492/149‑1 (MQ=255)
                                                                                ccGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCGTCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGaa                                                                         <  7:22749/141‑1 (MQ=255)
                                                                                ccGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCGTCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGaa                                                                         >  8:28102/1‑141 (MQ=255)
                                                                                ccGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCGTCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGaa                                                                         <  7:28102/141‑1 (MQ=255)
                                                                                ccGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCGTCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGaa                                                                         >  8:22749/1‑141 (MQ=255)
                                                                                ccGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCTCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTg                                                                <  7:116311/149‑1 (MQ=255)
                                                                                ccGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTg                                                                >  6:56980/1‑149 (MQ=255)
                                                                                      gTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGc                                                            >  7:264230/1‑147 (MQ=255)
                                                                                         cGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGc                                                        >  4:174429/1‑148 (MQ=255)
                                                                                         cGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGc                                                        <  3:174429/148‑1 (MQ=255)
                                                                                            cGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCtt                                                    >  1:317594/1‑149 (MQ=255)
                                                                                             ggCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTg                                                   <  8:14135/149‑1 (MQ=255)
                                                                                              gCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTgg                                                  <  5:56980/149‑1 (MQ=255)
                                                                                               cccAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTcgc                                                                                                         >  2:22710/1‑94 (MQ=255)
                                                                                               cccAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTcgc                                                                                                         <  1:22710/94‑1 (MQ=255)
                                                                                                ccAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGgcg                                                >  7:10084/1‑149 (MQ=255)
                                                                                                      ttCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATAGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCAGCCGCAAGCCCTAGgctccggaa                                          >  4:228011/1‑142 (MQ=255)
                                                                                                         aGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATgg                                       <  4:139683/149‑1 (MQ=255)
                                                                                                          gATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATc                                                                                                    <  5:68769/88‑1 (MQ=255)
                                                                                                          gATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATc                                                                                                    >  6:68769/1‑88 (MQ=255)
                                                                                                          gATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGt                                      <  6:196703/149‑1 (MQ=255)
                                                                                                          gATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGt                                      >  6:246903/1‑149 (MQ=255)
                                                                                                            tCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGt                                      >  3:237264/1‑147 (MQ=255)
                                                                                                            tCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGt                                      <  4:237264/147‑1 (MQ=255)
                                                                                                            tCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTc                                     >  3:112994/1‑148 (MQ=255)
                                                                                                              aGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGAt                                  <  3:228011/149‑1 (MQ=255)
                                                                                                               gggAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATc                                 <  8:264230/149‑1 (MQ=255)
                                                                                                               gcgagaGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGAt                                  <  1:307324/146‑1 (MQ=255)
                                                                                                                ggAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCa                                <  2:284616/149‑1 (MQ=255)
                                                                                                                       cGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCtt                         <  5:246903/149‑1 (MQ=255)
                                                                                                                         gTGGATGTCCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGa                       <  5:133636/149‑1 (MQ=255)
                                                                                                                                aCCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGcagcag                >  3:267269/1‑149 (MQ=255)
                                                                                                                                aCCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGcagcag                <  2:317594/149‑1 (MQ=255)
                                                                                                                                  ctggctggGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAATCACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGCAGCAgg               <  1:99042/149‑1 (MQ=255)
                                                                                                                                  ctggctggGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGa                       >  2:279395/1‑140 (MQ=255)
                                                                                                                                  ctggctggGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGa                       <  1:279395/140‑1 (MQ=255)
                                                                                                                                          gATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTc                                     >  3:223955/1‑118 (MQ=255)
                                                                                                                                          gATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTc                                     <  4:223955/118‑1 (MQ=255)
                                                                                                                                          gATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGCAGCAGGCGACCCAAg      >  8:174483/1‑149 (MQ=255)
                                                                                                                                              atcCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGCAGCAGGCGACCCAAGCCat  <  3:9785/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
TTGCCGTAGAGTTTTTCCATCAATTTCTTCGAAGGGCCGTTCATGGGGAGCCTCCCGTAATCGGGCCGCCGCGCATTGACCCGTCAGTGCGACGGCCCAGGGTTCAGATCAGGGAGAGCCGGTGGATGACCTGGCTGGGATCATCCGGGTCAGCGGCGGTGGTGAATCCTGCCGCCTTGGCCAGCTCGCGCATCGCTTGGTTGGCCGCTGCGTCGACCGAAT‑ACATCTGCCGCAAGCCCTTGGCGCGGGCATGGTCGATCAGGTGCTTGAGCAGCAGGCGACCCAAGCCAT  >  NC_002947/3668643‑3668933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑