Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,674,476 T→C 7.1% Q27R (CAG→CGG)  PP_3238 ← transcriptional regulator PyrR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,674,4760TC 7.1% 156.2 / 2.8 56Q27R (CAG→CGG) PP_3238transcriptional regulator PyrR
Reads supporting (aligned to +/- strand):  ref base T (27/25);  new base C (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

GCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGA  >  NC_002947/3674331‑3674622
                                                                                                                                                 |                                                                                                                                                     
gcgcTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGc                                                                                                                                                    <  4:266424/149‑1 (MQ=255)
  gcTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCgg                                                                                                                                                  <  6:105780/149‑1 (MQ=255)
  gcTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCgg                                                                                                                                                  <  2:266134/149‑1 (MQ=255)
         cTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCCGCCAGGCCAtgg                                                                                                                                           <  1:91616/149‑3 (MQ=255)
         cTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAAAGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑c                                                                                                                                           >  4:73387/1‑148 (MQ=255)
            gATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAg                                                                                                                                        <  1:17482/148‑1 (MQ=255)
            gATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAg                                                                                                                                        >  2:17482/1‑148 (MQ=255)
             aTCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAACGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAg‑‑c                                                                                                                                     <  3:73387/148‑1 (MQ=255)
                 ggTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAAGCAAGCAGGCCATGGCCCGCCAGGCCAtggcct                                                                                                                                        <  2:298563/144‑6 (MQ=255)
                 ggTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGAAAGCAAGCAGGCCATGGCCCGCCAGGCCAtggcct                                                                                                                                        >  1:298563/1‑139 (MQ=255)
                  gTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑Cgcagca                                                                                                                               >  5:301805/1‑149 (MQ=255)
                        cGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGAGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAC‑‑CGCAGCACACTGt                                                                                                                         >  4:326064/1‑149 (MQ=255)
                                gcgcAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATc                                                                                                                  <  3:52639/148‑1 (MQ=255)
                                          gTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCGAGATCGTCTTCGTCa                                                                                                        >  8:98623/1‑148 (MQ=255)
                                              aGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAg                                                                                                   >  4:285659/1‑149 (MQ=255)
                                                        ggTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAAc                                                                                             <  3:28449/145‑1 (MQ=255)
                                                        ggTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAAc                                                                                             >  4:28449/1‑145 (MQ=255)
                                                                    ggCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCgggg                                                                             >  4:130726/1‑149 (MQ=255)
                                                                     gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg                                                                                >  2:79873/1‑145 (MQ=255)
                                                                     gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg                                                                                >  2:84770/1‑145 (MQ=255)
                                                                     gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg                                                                                <  1:79873/145‑1 (MQ=255)
                                                                     gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg                                                                                <  1:84770/145‑1 (MQ=255)
                                                                       gcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGa                                                                          >  7:103594/1‑149 (MQ=255)
                                                                           cgcCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTc                                                                       <  3:130726/148‑1 (MQ=255)
                                                                           cgcCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTc                                                                       <  6:301805/148‑1 (MQ=255)
                                                                             ccAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATg                                                                    <  5:277198/149‑1 (MQ=255)
                                                                             ccAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATg                                                                    >  7:31675/1‑149 (MQ=255)
                                                                                       accacGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTcc                                                          >  8:116518/1‑149 (MQ=255)
                                                                                        ccacGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCATGGGAGACCGCACCACAGGGACAACATCGACTTCTAAATAACGCCGAACCCGGGTAGCAGCGGGCCGATTCATGTTGtttt                                                             >  1:290520/1‑148 (MQ=255)
                                                                                          acGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCttt                                                       >  7:62946/1‑149 (MQ=255)
                                                                                            gccgcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTAt                                                     <  3:232305/149‑1 (MQ=255)
                                                                                            gccgcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTAt                                                     <  3:285659/149‑1 (MQ=255)
                                                                                              cgcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTg                                                   <  7:116518/149‑1 (MQ=255)
                                                                                               gcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTgg                                                  <  1:165220/149‑1 (MQ=255)
                                                                                                cTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGa                                                 >  8:51472/1‑149 (MQ=255)
                                                                                                  gAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCa                                                                                                        >  4:259397/1‑92 (MQ=255)
                                                                                                  gAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCa                                                                                                        <  3:259397/92‑1 (MQ=255)
                                                                                                         tACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCa                                            <  3:38066/145‑1 (MQ=255)
                                                                                                         tACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCa                                            >  4:38066/1‑145 (MQ=255)
                                                                                                         tACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCActct                                        >  7:187255/1‑149 (MQ=255)
                                                                                                          aCCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGtttt                                                             <  1:21951/127‑1 (MQ=255)
                                                                                                          aCCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGACTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGtttt                                                             >  2:21951/1‑127 (MQ=255)
                                                                                                           ccAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGat                                                <  2:112610/139‑1 (MQ=255)
                                                                                                           ccAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGat                                                >  1:112610/1‑139 (MQ=255)
                                                                                                            cAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAT‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTGATTGTGTTTTTCCTTTATTGGATATCACTCTGtt                                     <  3:326064/149‑1 (MQ=255)
                                                                                                                    cTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGcc                             <  8:62946/149‑1 (MQ=255)
                                                                                                                            gTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGCGCCTACCCggg                     >  7:133305/1‑149 (MQ=255)
                                                                                                                              aGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTc                                    >  2:113827/1‑132 (MQ=255)
                                                                                                                              aGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCCTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTc                                    <  1:113827/132‑1 (MQ=255)
                                                                                                                                  aaGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCtt               >  7:250279/1‑149 (MQ=255)
                                                                                                                                   aGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGa                                <  3:190736/131‑1 (MQ=255)
                                                                                                                                   aGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGa                                >  4:190736/1‑131 (MQ=255)
                                                                                                                                        tcgaTGGCCCGCCAGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTg         >  1:343987/4‑149 (MQ=255)
                                                                                                                                         ccATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTgg        >  7:2807/1‑149 (MQ=255)
                                                                                                                                          cATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTggg       <  8:31675/149‑1 (MQ=255)
                                                                                                                                                cTGGAGGGCCAT‑CGAG‑‑CGCCGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGa  >  6:179035/1‑148 (MQ=255)
                                                                                                                                                 tGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCttt                                                       >  7:137427/1‑94 (MQ=255)
                                                                                                                                                 tGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCttt                                                       <  8:137427/94‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGA  >  NC_002947/3674331‑3674622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑