| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,674,478 | G→C | 6.9% | R26R (CGC→CGG) | PP_3238 ← | transcriptional regulator PyrR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,674,478 | 0 | G | C | 6.9% | 183.3 / 2.9 | 58 | R26R (CGC→CGG) | PP_3238 | transcriptional regulator PyrR |
| Reads supporting (aligned to +/- strand): ref base G (28/26); new base C (2/2); total (30/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01 | |||||||||||
GCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGATAAA > NC_002947/3674331‑3674626 | gcgcTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGc < 4:266424/149‑1 (MQ=255) gcTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCgg < 6:105780/149‑1 (MQ=255) gcTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCgg < 2:266134/149‑1 (MQ=255) cTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCCGCCAGGCCAtgg < 1:91616/149‑3 (MQ=255) cTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAAAGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑c > 4:73387/1‑148 (MQ=255) gATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAg < 1:17482/148‑1 (MQ=255) gATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAg > 2:17482/1‑148 (MQ=255) aTCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAACGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAg‑‑c < 3:73387/148‑1 (MQ=255) ggTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAAGCAAGCAGGCCATGGCCCGCCAGGCCAtggcct < 2:298563/144‑6 (MQ=255) ggTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGAAAGCAAGCAGGCCATGGCCCGCCAGGCCAtggcct > 1:298563/1‑139 (MQ=255) gTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑Cgcagca > 5:301805/1‑149 (MQ=255) cGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGAGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAC‑‑CGCAGCACACTGt > 4:326064/1‑149 (MQ=255) gcgcAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATc < 3:52639/148‑1 (MQ=255) gTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCGAGATCGTCTTCGTCa > 8:98623/1‑148 (MQ=255) aGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAg > 4:285659/1‑149 (MQ=255) ggTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAAc < 3:28449/145‑1 (MQ=255) ggTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAAc > 4:28449/1‑145 (MQ=255) ggCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCgggg > 4:130726/1‑149 (MQ=255) gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg > 2:79873/1‑145 (MQ=255) gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg > 2:84770/1‑145 (MQ=255) gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg < 1:79873/145‑1 (MQ=255) gcgcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCg < 1:84770/145‑1 (MQ=255) gcTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGa > 7:103594/1‑149 (MQ=255) cgcCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTc < 3:130726/148‑1 (MQ=255) cgcCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTc < 6:301805/148‑1 (MQ=255) ccAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATg < 5:277198/149‑1 (MQ=255) ccAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATg > 7:31675/1‑149 (MQ=255) accacGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTcc > 8:116518/1‑149 (MQ=255) ccacGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCATGGGAGACCGCACCACAGGGACAACATCGACTTCTAAATAACGCCGAACCCGGGTAGCAGCGGGCCGATTCATGTTGtttt > 1:290520/1‑148 (MQ=255) acGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCttt > 7:62946/1‑149 (MQ=255) gccgcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTAt < 3:232305/149‑1 (MQ=255) gccgcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTAt < 3:285659/149‑1 (MQ=255) cgcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTg < 7:116518/149‑1 (MQ=255) gcTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTgg < 1:165220/149‑1 (MQ=255) cTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGa > 8:51472/1‑149 (MQ=255) gAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCa > 4:259397/1‑92 (MQ=255) gAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCa < 3:259397/92‑1 (MQ=255) tACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCa < 3:38066/145‑1 (MQ=255) tACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCa > 4:38066/1‑145 (MQ=255) tACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCActct > 7:187255/1‑149 (MQ=255) aCCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGtttt < 1:21951/127‑1 (MQ=255) aCCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGACTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGtttt > 2:21951/1‑127 (MQ=255) ccAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGat < 2:112610/139‑1 (MQ=255) ccAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGat > 1:112610/1‑139 (MQ=255) cAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAT‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTGATTGTGTTTTTCCTTTATTGGATATCACTCTGtt < 3:326064/149‑1 (MQ=255) cTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGcc < 8:62946/149‑1 (MQ=255) gTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGCGCCTACCCggg > 7:133305/1‑149 (MQ=255) aGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTc > 2:113827/1‑132 (MQ=255) aGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCCTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTc < 1:113827/132‑1 (MQ=255) aaGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCtt > 7:250279/1‑149 (MQ=255) aGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGa < 3:190736/131‑1 (MQ=255) aGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGa > 4:190736/1‑131 (MQ=255) tcgaTGGCCCGCCAGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTg > 1:343987/4‑149 (MQ=255) ccATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTgg > 7:2807/1‑149 (MQ=255) cATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTggg < 8:31675/149‑1 (MQ=255) cTGGAGGGCCAT‑CGAG‑‑CGCCGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGa > 6:179035/1‑148 (MQ=255) tGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCttt > 7:137427/1‑94 (MQ=255) tGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCttt < 8:137427/94‑1 (MQ=255) gCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGATaaa < 8:2807/149‑1 (MQ=255) | GCGCTTGACCTTGATCGGGTAGAGCGGCAATGGCGCAGCACCGTTCAGTGCTTGCAGGTGCAGTTGCAGGCGCTGCGCCAGCGGCTCACCACGCCGCTGAATGCCTACCAGCGCGACTTCAGACGTAGGCAAGCAGGCCATGGCCTGGCGGGCCAT‑CGAG‑‑CGCAGCACACTGTCCAGATCGTCTTCGTCATACAGGCGAACCCTCGATGGAGCGGGGCGATTCATGGTGTTTTTCCTTTATTGGATATCACTCTGTTCGGGAGCCTACCCGGGCACCTTGAGGTGGGCTTGATAAA > NC_002947/3674331‑3674626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |