Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,097 T→G 11.6% M166L (ATG→CTG) ‡ paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,0970TG11.6% 162.2 / 11.2 59M166L (ATG→CTG) ‡paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base T (25/27);  new base G (5/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTG  >  NC_002947/3717950‑3718245
                                                                                                                                                   |                                                                                                                                                      
gcTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATg                                                                                                                                                       >  5:56527/1‑149 (MQ=255)
     aTCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAg                                                                                                                                                  <  4:99470/149‑1 (MQ=255)
         gtgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTACTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGcc                                                                                                                                              <  3:329195/149‑1 (MQ=255)
          tgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCt                                                                                                                                             <  6:56527/149‑1 (MQ=255)
                         atcttgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGc                                                                                                                              <  3:96505/145‑1 (MQ=255)
                            ctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGa                                                                                                                           <  1:157065/149‑1 (MQ=255)
                            ctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGa                                                                                                                           <  2:31017/149‑1 (MQ=255)
                              gggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGAc                                                                                                                          >  8:201185/1‑148 (MQ=255)
                              gggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGAGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCCTGc                                                                                                                              >  4:96505/1‑144 (MQ=255)
                                             gggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTTGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCagcagc                                                                                                          >  8:155172/1‑149 (MQ=255)
                                              ggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         <  6:308083/149‑1 (MQ=255)
                                              ggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         <  2:225312/149‑1 (MQ=255)
                                                        aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         <  3:265416/139‑1 (MQ=255)
                                                        aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         >  4:265416/1‑139 (MQ=255)
                                                        aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑ccgc‑c                                                                                             >  1:282538/1‑149 (MQ=255)
                                                            cTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAg                                                                                         >  1:3791/1‑149 (MQ=255)
                                                             ttCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGt                                                                                        >  8:232843/1‑149 (MQ=255)
                                                             ttCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGt                                                                                        >  8:237334/1‑149 (MQ=255)
                                                               catcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTcc                                                                                      >  6:154222/1‑149 (MQ=255)
                                                                atcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCg                                                                                     <  7:201185/149‑1 (MQ=255)
                                                                atcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCg                                                                                     >  5:153685/1‑149 (MQ=255)
                                                                    cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCAc                                                                                 >  7:262334/1‑149 (MQ=255)
                                                                    cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCAc                                                                                 >  2:212172/1‑149 (MQ=255)
                                                                         cgcTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGc                                                                            <  2:282538/149‑1 (MQ=255)
                                                                            tGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGGCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCgt                                                                         <  5:353221/149‑2 (MQ=255)
                                                                              catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGTGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATc                                                                       >  6:217002/1‑149 (MQ=255)
                                                                              catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATc                                                                       <  6:129562/149‑1 (MQ=255)
                                                                                tcatcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGTGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     <  5:217002/149‑1 (MQ=255)
                                                                                  atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGc                                                                   <  1:212172/149‑1 (MQ=255)
                                                                                          cgcgGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGCAGGcc                                                           >  5:19875/1‑149 (MQ=255)
                                                                                             ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                        <  7:232843/149‑1 (MQ=255)
                                                                                             ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                        <  7:237334/149‑1 (MQ=255)
                                                                                             ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTg                                                         >  5:169118/1‑148 (MQ=255)
                                                                                                   tCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGaaa                                                  >  6:246634/1‑149 (MQ=255)
                                                                                                        ccccATTGCTCGGCCTGTTTGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGc                                             <  7:155172/149‑1 (MQ=255)
                                                                                                        ccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGc                                             <  5:246634/149‑1 (MQ=255)
                                                                                                         cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     >  6:107782/1‑124 (MQ=255)
                                                                                                         cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     <  5:107782/124‑1 (MQ=255)
                                                                                                         cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                            <  6:153685/149‑1 (MQ=255)
                                                                                                         cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                            >  2:70323/1‑149 (MQ=255)
                                                                                                         cccATTGCTCGGCCAGATCGGCCCCAAGGCGATCGCCCAGCATGGAAAACGCCTTGGCCCGGGCCATGCCGACCAACCGCGGCAGCAGACAAGTA‑CCGC‑CGGAGTCCGGCACAAGGACGATATTGCAGAAGGCCAGGATGAAACTGGcc                                            >  8:333596/1‑149 (MQ=255)
                                                                                                             ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGGGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                                        <  2:353137/149‑1 (MQ=255)
                                                                                                             ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                                        <  8:262334/149‑1 (MQ=255)
                                                                                                                     ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                                 >  2:62439/1‑148 (MQ=255)
                                                                                                                      atggcccgcgcCAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGCCCAACCGCGGCAGCAGCCAAGTA‑CCGC‑CGCAGTCCGGCACCACGCCGAAATTGCAGAAGGCCAGGATGAAACTGGCCGAGCGGGCGGCCa                               >  6:109488/8‑149 (MQ=25)
                                                                                                                                 cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCGCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                    >  1:328968/1‑149 (MQ=38)
                                                                                                                                 cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGc                                                                   <  4:117634/102‑1 (MQ=37)
                                                                                                                                 cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGc                                                                   >  3:117634/1‑102 (MQ=37)
                                                                                                                                   aGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCAGACCAAGCGCGGCAGCAGCCAAGTAGGAGCACGGAGTCCCGCACCAAGCCGATATTGCAGAAGCCCTGGATGAAACTGGCCGCGCGGGCGGCCAGCACCAAGTc                     >  1:290286/1‑148 (MQ=255)
                                                                                                                                    ggCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCt                                                                      >  6:69791/1‑96 (MQ=37)
                                                                                                                                    ggCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCt                                                                      <  5:69791/96‑1 (MQ=37)
                                                                                                                                     gcgcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAgg                >  5:247421/1‑149 (MQ=255)
                                                                                                                                          cGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg           <  7:333596/149‑1 (MQ=255)
                                                                                                                                            cccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAggg         <  5:154222/149‑1 (MQ=255)
                                                                                                                                               aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     >  7:183544/1‑86 (MQ=255)
                                                                                                                                               aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg           >  5:275730/1‑144 (MQ=255)
                                                                                                                                               aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg           <  6:275730/144‑1 (MQ=255)
                                                                                                                                               aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAAt      >  7:120670/1‑149 (MQ=255)
                                                                                                                                               aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGCCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     <  8:183544/86‑1 (MQ=255)
                                                                                                                                                   tGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTg  <  6:169118/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GCTTGATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTG  >  NC_002947/3717950‑3718245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑