Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,718,101 C→G 11.6% L164L (CTG→CTC)  paaG ← 2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,718,1010CG11.6% 167.6 / 10.2 59L164L (CTG→CTC) paaG2‑(1,2‑epoxy‑1,2‑dihydrophenyl)acetyl‑CoA isomerase
Reads supporting (aligned to +/- strand):  ref base C (23/28);  new base G (5/2);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTG  >  NC_002947/3717955‑3718245
                                                                                                                                                  |                                                                                                                                                  
aTCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAg                                                                                                                                                  <  4:99470/149‑1 (MQ=255)
    gtgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTACTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGcc                                                                                                                                              <  3:329195/149‑1 (MQ=255)
     tgtCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCt                                                                                                                                             <  6:56527/149‑1 (MQ=255)
                    atcttgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGc                                                                                                                              <  3:96505/145‑1 (MQ=255)
                       ctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGa                                                                                                                           <  1:157065/149‑1 (MQ=255)
                       ctgggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGa                                                                                                                           <  2:31017/149‑1 (MQ=255)
                         gggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGAc                                                                                                                          >  8:201185/1‑148 (MQ=255)
                         gggcGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGAGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCCTGc                                                                                                                              >  4:96505/1‑144 (MQ=255)
                                        gggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTTGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCagcagc                                                                                                          >  8:155172/1‑149 (MQ=255)
                                         ggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         <  2:225312/149‑1 (MQ=255)
                                         ggcgAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         <  6:308083/149‑1 (MQ=255)
                                                   aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         <  3:265416/139‑1 (MQ=255)
                                                   aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGcc                                                                                                         >  4:265416/1‑139 (MQ=255)
                                                   aGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑ccgc‑c                                                                                             >  1:282538/1‑149 (MQ=255)
                                                       cTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAg                                                                                         >  1:3791/1‑149 (MQ=255)
                                                        ttCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGt                                                                                        >  8:232843/1‑149 (MQ=255)
                                                        ttCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGt                                                                                        >  8:237334/1‑149 (MQ=255)
                                                          catcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTcc                                                                                      >  6:154222/1‑149 (MQ=255)
                                                           atcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCg                                                                                     >  5:153685/1‑149 (MQ=255)
                                                           atcaCGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCg                                                                                     <  7:201185/149‑1 (MQ=255)
                                                               cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCAc                                                                                 >  7:262334/1‑149 (MQ=255)
                                                               cGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCAc                                                                                 >  2:212172/1‑149 (MQ=255)
                                                                    cgcTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGc                                                                            <  2:282538/149‑1 (MQ=255)
                                                                       tGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGGCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCgt                                                                         <  5:353221/149‑2 (MQ=255)
                                                                         catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGTGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATc                                                                       >  6:217002/1‑149 (MQ=255)
                                                                         catcatCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATc                                                                       <  6:129562/149‑1 (MQ=255)
                                                                           tcatcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGTGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     <  5:217002/149‑1 (MQ=255)
                                                                             atcCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGc                                                                   <  1:212172/149‑1 (MQ=255)
                                                                                     cgcgGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGCAGGcc                                                           >  5:19875/1‑149 (MQ=255)
                                                                                        ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                        <  7:232843/149‑1 (MQ=255)
                                                                                        ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTgg                                                        <  7:237334/149‑1 (MQ=255)
                                                                                        ggTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTg                                                         >  5:169118/1‑148 (MQ=255)
                                                                                              tCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGaaa                                                  >  6:246634/1‑149 (MQ=255)
                                                                                                   ccccATTGCTCGGCCTGTTTGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGc                                             <  7:155172/149‑1 (MQ=255)
                                                                                                   ccccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGc                                             <  5:246634/149‑1 (MQ=255)
                                                                                                    cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     >  6:107782/1‑124 (MQ=255)
                                                                                                    cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     <  5:107782/124‑1 (MQ=255)
                                                                                                    cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                            <  6:153685/149‑1 (MQ=255)
                                                                                                    cccATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGcc                                            >  2:70323/1‑149 (MQ=255)
                                                                                                    cccATTGCTCGGCCAGATCGGCCCCAAGGCGATCGCCCAGCATGGAAAACGCCTTGGCCCGGGCCATGCCGACCAACCGCGGCAGCAGACAAGTA‑CCGC‑CGGAGTCCGGCACAAGGACGATATTGCAGAAGGCCAGGATGAAACTGGcc                                            >  8:333596/1‑149 (MQ=255)
                                                                                                        ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGGGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                                        <  2:353137/149‑1 (MQ=255)
                                                                                                        ttGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAgc                                        <  8:262334/149‑1 (MQ=255)
                                                                                                                ccTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGggcggc                                 >  2:62439/1‑148 (MQ=255)
                                                                                                                 atggcccgcgcCAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGCCCAACCGCGGCAGCAGCCAAGTA‑CCGC‑CGCAGTCCGGCACCACGCCGAAATTGCAGAAGGCCAGGATGAAACTGGCCGAGCGGGCGGCCa                               >  6:109488/8‑149 (MQ=25)
                                                                                                                            cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCGCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCg                    >  1:328968/1‑149 (MQ=38)
                                                                                                                            cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGc                                                                   <  4:117634/102‑1 (MQ=37)
                                                                                                                            cAAGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGc                                                                   >  3:117634/1‑102 (MQ=37)
                                                                                                                              aGGCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCAGACCAAGCGCGGCAGCAGCCAAGTAGGAGCACGGAGTCCCGCACCAAGCCGATATTGCAGAAGCCCTGGATGAAACTGGCCGCGCGGGCGGCCAGCACCAAGTc                     >  1:290286/1‑148 (MQ=255)
                                                                                                                               ggCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCt                                                                      >  6:69791/1‑96 (MQ=37)
                                                                                                                               ggCGCTGGCCATGCTGGGCGAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCt                                                                      <  5:69791/96‑1 (MQ=37)
                                                                                                                                gcgcTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAgg                >  5:247421/1‑149 (MQ=255)
                                                                                                                                     cGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg           <  7:333596/149‑1 (MQ=255)
                                                                                                                                       cccAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAggg         <  5:154222/149‑1 (MQ=255)
                                                                                                                                          aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     >  7:183544/1‑86 (MQ=255)
                                                                                                                                          aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg           <  6:275730/144‑1 (MQ=255)
                                                                                                                                          aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAg           >  5:275730/1‑144 (MQ=255)
                                                                                                                                          aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAAt      >  7:120670/1‑149 (MQ=255)
                                                                                                                                          aGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGCCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCtt                                                                     <  8:183544/86‑1 (MQ=255)
                                                                                                                                              tGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTg  <  6:169118/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATCAGTGTCAGGCCGTAGGTGGGCTGGGCGGCGAGGTGGCGGGCGAGGGTGAGGGCTTCATCACGCAGCGCTGCATCATCCACCACGCGGTAGATCAGCCCCCATTGCTCGGCCTGTTCGGCGCCAAGGCGCTCGCCCAGCATGGCCAGCGCCTTGGCCCGGGCCATGCCGACCAAGCGCGGCAGCAGCCAAGTA‑CCGC‑CGGAGTCCGGCACCAGGCCGATCTTGCAGAAGGCCTGGATGAAACTGGCCGAGCGGGCGGCCAGCACCAGGTCGCAGGCCAAGGGAATGTTG  >  NC_002947/3717955‑3718245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑