Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,738,813	A→G	5.6%	Q189R (CAG→CGG)	PP_3303 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,738,813	0	A	G	5.6%	209.9 / 2.4	71	Q189R (CAG→CGG)	PP_3303	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base A (37/30);  new base G (2/2);  total (39/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

CACCACTGACGCACGTGGCCCTCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAGCCCTGA  >  NC_002947/3738667‑3738960                                                                                                                                                    |                                                                                                                                                    caccacTGACGCACGTGGCCCTCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAgg                                                                                                                                                   >  3:272660/1‑149 (MQ=255)    cacTGACGCACGTGGCCCTCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCa                                                                                                                                                <  5:330997/149‑1 (MQ=255)        gACGCACGTGGCCCTCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAg                                                                                                                                                    <  3:36868/141‑1 (MQ=255)        gACGCACGTGGCCCTCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAg                                                                                                                                                    >  4:36868/1‑141 (MQ=255)         aCGCACGTGGCCCTCTCAAGTTGTCGCCCTTCACTGCTCCATCGTTCCTTGCCAATATGGCAGC‑GGGTCATGTCTCCATACTCTATGTCTTCGAATGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTg                                                                                                                                             <  8:225179/147‑1 (MQ=255)                      tCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGc                                                                                                                              >  1:27160/1‑149 (MQ=255)                        gCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGc                                                                                                                              <  5:48140/147‑1 (MQ=255)                                 cGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCtcat                                                                                                                          <  1:246710/142‑1 (MQ=255)                                 cGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCtcat                                                                                                                          >  2:246710/1‑142 (MQ=255)                                  gCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAgcg                                                                                                                  >  5:70678/1‑149 (MQ=255)                                    ccTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAgcggc                                                                                                                <  4:272660/149‑1 (MQ=255)                                          cGGCGCCATCGTTCCTGGCCAATATGGCAGCGGGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCtc                                                                                                                            >  1:54269/1‑132 (MQ=255)                                          cGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCtc                                                                                                                            <  2:54269/131‑1 (MQ=255)                                          cGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCCCTGCGGGGGTGCAGGCCAATGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCg                                                                                                          >  3:109224/1‑149 (MQ=255)                                                cATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATc                                                                                                    >  7:192608/1‑149 (MQ=255)                                                 aTCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATcg                                                                                                   <  4:41723/149‑1 (MQ=255)                                                 aTCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATcg                                                                                                   >  4:159204/1‑149 (MQ=255)                                                 aTCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATcg                                                                                                   >  3:41723/1‑149 (MQ=255)                                                        cTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTg                                                                                            >  4:250066/1‑149 (MQ=255)                                                           gCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAgcgg                                                                                                                 >  5:28087/1‑125 (MQ=255)                                                           gCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAgcgg                                                                                                                 <  6:28087/125‑1 (MQ=255)                                                           gCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATc                                                                                                    >  6:73676/1‑138 (MQ=255)                                                           gCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATc                                                                                                    <  5:73676/138‑1 (MQ=255)                                                           gCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCgg                                                                                         >  3:108760/1‑149 (MQ=255)                                                               atatGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGgc                                                                                     >  1:184475/1‑149 (MQ=255)                                                               atatGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACACCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGgc                                                                                     >  4:147930/1‑149 (MQ=255)                                                                    gCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCg                                                                                 <  2:184475/148‑1 (MQ=255)                                                                      aGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAgg                                                                              >  4:90326/1‑149 (MQ=255)                                                                             tCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCaa                                                                        >  7:316683/1‑149 (MQ=255)                                                                               aggTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGTCGACGCCGCTCGGCGCATCAGAAGCGGCGAAGCGGATCTCGCTATCTGCGGTGGCGCCGAGGCGGCAAtt                                                                      <  8:165352/147‑1 (MQ=255)                                                                                    tcCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCt                                                                                             <  8:326857/121‑1 (MQ=255)                                                                                    tcCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCt                                                                                             >  7:326857/1‑121 (MQ=255)                                                                                      cATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtg                                                               >  5:16700/1‑149 (MQ=255)                                                                                      cATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtg                                                               >  3:89668/1‑149 (MQ=255)                                                                                      cATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACACCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATc                                                                  <  3:147930/146‑1 (MQ=255)                                                                                       aTACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtgt                                                              >  8:49301/1‑149 (MQ=255)                                                                                       aTACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtgt                                                              >  8:199129/1‑149 (MQ=255)                                                                                       aTACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtgt                                                              <  3:159204/149‑1 (MQ=255)                                                                                           gCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtgt                                                              <  1:28071/145‑1 (MQ=255)                                                                                           gCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCgtgt                                                              >  2:28071/1‑145 (MQ=255)                                                                                             tATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGc                                                         >  3:101625/1‑148 (MQ=255)                                                                                               tGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTa                                                       <  2:3816/148‑1 (MQ=255)                                                                                                 gCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCGGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGa                                                                    <  1:284512/133‑1 (MQ=255)                                                                                                 gCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCGGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGa                                                                    <  1:144061/133‑1 (MQ=255)                                                                                                 gCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCGGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGa                                                                    >  2:144061/1‑133 (MQ=255)                                                                                                 gCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCGGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGa                                                                    >  2:284512/1‑133 (MQ=255)                                                                                                  cTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCCTCAGAAGCGGCGAAGCGGATATCGCGCTCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGCCGCTAGgct                                                   >  3:220758/1‑149 (MQ=255)                                                                                                   ttCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGgctg                                                  >  6:162325/1‑149 (MQ=255)                                                                                                       aaGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCttt                                              >  7:37400/1‑149 (MQ=255)                                                                                                            ccACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATGGTGTGACGCTAGGCTGCTTTGCGGc                                         >  3:264390/1‑149 (MQ=255)                                                                                                            ccACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGc                                         >  3:1744/1‑149 (MQ=255)                                                                                                            ccACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGc                                         <  8:192608/149‑1 (MQ=255)                                                                                                            ccACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGACATTGGCGACGCCGCTCGGCTCATCAGAAGCGTCGAAGCGGATATCGCGATCTGCTGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGc                                         >  5:366221/1‑149 (MQ=255)                                                                                                                   gggCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGa                                  <  7:199129/149‑1 (MQ=255)                                                                                                                     ggacgcGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCGGCTCGGCTCATCTGAAGGGGCGAAGCGGTTATCGCGATCTGCGGTGGCGCCGTGGGTGCAATTGATCGTGTGACGCTAGgct                                                   <  7:276522/125‑1 (MQ=255)                                                                                                                     gCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGgct                                                   >  8:276522/1‑130 (MQ=255)                                                                                                                         ccGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCt                             >  4:118055/1‑148 (MQ=255)                                                                                                                         ccGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTc                            <  6:70678/149‑1 (MQ=255)                                                                                                                              acaGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCAc                       >  2:330241/1‑149 (MQ=255)                                                                                                                              acaGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCAc                       <  7:49301/149‑1 (MQ=255)                                                                                                                                 gCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACggg                    <  8:316683/149‑1 (MQ=255)                                                                                                                                 gCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACggg                    >  8:284600/1‑149 (MQ=255)                                                                                                                                   ctgcgctgcgGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCt                  >  1:38996/1‑149 (MQ=255)                                                                                                                                       gctgcgGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTc              <  4:89668/149‑1 (MQ=255)                                                                                                                                           cgGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGa           <  3:118055/148‑1 (MQ=255)                                                                                                                                           cgGGGGTGCAGGCCAATGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACTCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGTGCTTTTCGGa           <  4:109224/148‑1 (MQ=255)                                                                                                                                             ggggTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAg        <  4:264390/149‑1 (MQ=255)                                                                                                                                                 tGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAGCCCt    <  7:284600/149‑1 (MQ=255)                                                                                                                                                 tGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAGCCCt    <  6:16700/149‑1 (MQ=255)                                                                                                                                                   cAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAGCCCt    >  3:153624/1‑147 (MQ=255)                                                                                                                                                   cAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAGCCCTGa  <  3:250066/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CACCACTGACGCACGTGGCCCTCGCAAGTTGTCGCCCTTCACGGCGCCATCGTTCCTGGCCAATATGGCAGC‑GGGTCATGTCTCCATACGCTATGGCTTCGAAGGCCCACTTGGGGCACCCGTCACAGCCTGCGCTGCGGGGGTGCAGGCCATTGGCGACGCCGCTCGGCTCATCAGAAGCGGCGAAGCGGATATCGCGATCTGCGGTGGCGCCGAGGCGGCAATTGATCGTGTGACGCTAGGCTGCTTTGCGGCTGCACGAGCGCTCAGCACGGGCTTTTCGGATAGCCCTGA  >  NC_002947/3738667‑3738960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑