Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,759,984 T→C 9.1% L206P (CTG→CCG) ‡ folEB → GTP cyclohydrolase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,759,9840TC 9.1% 180.0 / 7.1 67L206P (CTG→CCG) ‡folEBGTP cyclohydrolase I
Reads supporting (aligned to +/- strand):  ref base T (33/27);  new base C (3/3);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GAGGTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCTG  >  NC_002947/3759836‑3760127
                                                                                                                                                    |                                                                                                                                                
gAGGTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTACGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCt                                                                                                                                                  <  8:327693/149‑1 (MQ=255)
  ggTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTgg                                                                                                                                                <  1:17544/149‑1 (MQ=255)
         aTCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGcc                                                                                                                                         <  5:64206/149‑1 (MQ=255)
         aTCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcg                                                                                                                                         <  7:130764/149‑2 (MQ=255)
          tCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCca                                                                                                                                        <  4:333507/149‑1 (MQ=255)
             gCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCcaca                                                                                                                                      >  2:350808/1‑148 (MQ=255)
              cTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcgttgct                                                                                                                                    >  4:71091/1‑143 (MQ=255)
               tACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGa                                                                                                                                             <  4:316669/139‑1 (MQ=255)
               tACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGa                                                                                                                                             >  3:316669/1‑139 (MQ=255)
               tACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAg                                                                                                                                   >  2:73241/1‑149 (MQ=255)
                            gCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCg                                                                                                                        >  8:101223/1‑147 (MQ=255)
                             cccGTGCTCGGCCGCTCTCTCCCTGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGGAGCGaa                                                                                                                      <  7:227242/148‑1 (MQ=255)
                             cccGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAg                                                                                                                     >  5:29017/1‑149 (MQ=255)
                                   cTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTc                                                                                                                  <  4:100061/146‑1 (MQ=255)
                                   cTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTc                                                                                                                  >  3:100061/1‑146 (MQ=255)
                                    tCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcgttgct                                                                                                                                    <  7:262986/127‑7 (MQ=255)
                                    tCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcgttgct                                                                                                                                    >  8:262986/1‑121 (MQ=255)
                                        ccGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTgcgc                                                                                                          <  2:25151/149‑1 (MQ=255)
                                          gCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCCGCAACGCGTCGATGGCGACGCCACATAGCCACCGCAGCGAAGCTCGAGTGCGCGt                                                                                                        >  4:183538/1‑149 (MQ=255)
                                              tctcCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCTACATAGCCAACGCAGCGAACCTCGAGTGCGCGTCACg                                                                                                    >  6:314814/1‑149 (MQ=255)
                                              tctcCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCCCGTCACg                                                                                                    >  6:5471/1‑149 (MQ=255)
                                               ctcCCGGCAGTTGATTGAGGACAGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACgg                                                                                                   >  8:183705/1‑149 (MQ=255)
                                                tctccacAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACg                                                                                                    <  8:6344/141‑1 (MQ=255)
                                                  ccGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTa                                                                                                 <  5:64882/148‑1 (MQ=255)
                                                   cGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTAcc                                                                                               >  5:63112/1‑149 (MQ=255)
                                                           gattgaGGAAGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGCAGCACGAGTGCGCGTCACGGTACCTGCCGATg                                                                                       >  8:322296/1‑149 (MQ=255)
                                                               gaGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACTTAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGAAACCTGCCGATGcccc                                                                                   >  1:122583/1‑149 (MQ=255)
                                                                aGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGCCGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCACCg                                                                                  >  1:213311/1‑149 (MQ=255)
                                                                       cTTCCTGCGAACCTTCATGGCCCAGCCACTGCGCGACGTCAGCGCGGGCGCGCTGGGGCGGCGCAGCACCGCGGCGGGGGCGACGCCAAATCCCCACCGCCGCTACGCTGGTGGGCGGGTCAGGGTACCTGCCGATGCCCCGGATcgcg                                                                           <  2:149639/149‑1 (MQ=255)
                                                                       cTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCg                                                                           >  1:149639/1‑149 (MQ=255)
                                                                              cGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCggg                                                                         <  6:233187/144‑1 (MQ=255)
                                                                              cGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCggg                                                                         >  5:233187/1‑144 (MQ=255)
                                                                              cGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCg                                                                           >  4:282933/1‑142 (MQ=255)
                                                                              cGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCg                                                                           <  3:282933/142‑1 (MQ=255)
                                                                               gAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTg                                                                   <  7:322296/149‑1 (MQ=255)
                                                                               gAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTg                                                                   <  6:29017/149‑1 (MQ=255)
                                                                                   cTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGccc                                                               <  7:183705/149‑1 (MQ=255)
                                                                                   cTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGccc                                                               <  7:101223/149‑1 (MQ=255)
                                                                                       aaGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGAt                                                           <  1:73241/149‑1 (MQ=255)
                                                                                         ggACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCg                                                         <  5:5471/149‑1 (MQ=255)
                                                                                             cAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCa                                                     >  5:286881/1‑149 (MQ=255)
                                                                                             cAGCCACTGCGCGACGGCGCTGACTTCGAGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGGTGCTGGCCCTGATCGACCa                                                     >  8:336530/1‑149 (MQ=38)
                                                                                              aGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCAc                                                    <  2:165676/149‑1 (MQ=255)
                                                                                               gCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGCCGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACg                                                   >  6:335756/1‑149 (MQ=255)
                                                                                               gCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACg                                                   >  2:231681/1‑149 (MQ=255)
                                                                                                ccACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGt                                                  <  6:63112/149‑1 (MQ=255)
                                                                                                       tCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑gc                                          >  2:15691/1‑149 (MQ=255)
                                                                                                            gTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGTGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCtt                                     <  8:170687/149‑1 (MQ=255)
                                                                                                            gTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCtt                                     <  1:231681/149‑1 (MQ=255)
                                                                                                            gTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCtt                                     >  6:237369/1‑149 (MQ=255)
                                                                                                            gTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCtt                                     >  2:92367/1‑149 (MQ=255)
                                                                                                               aGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTgcg                                  >  1:267739/1‑149 (MQ=255)
                                                                                                                gcgcCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGcc                                                                <  6:160783/119‑1 (MQ=255)
                                                                                                                gcgcCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGAc                                                       <  3:70062/128‑1 (MQ=255)
                                                                                                                gcgcCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGAc                                                       >  4:70062/1‑128 (MQ=255)
                                                                                                                  gcCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCAc                               >  6:281883/1‑149 (MQ=255)
                                                                                                                         cgcAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGtt                        <  1:92367/149‑1 (MQ=255)
                                                                                                                          gcAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGaa                                              >  6:362120/1‑127 (MQ=255)
                                                                                                                          gcAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGaa                                              <  5:362120/127‑1 (MQ=255)
                                                                                                                             gTGGCTGCGCAGCAACGCGTCGCTGGCGACGCTACATAGCCTACGCCGCGAATCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGa                    <  5:314814/149‑1 (MQ=255)
                                                                                                                                gCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGAccg                 >  4:262645/1‑149 (MQ=255)
                                                                                                                                       tatgtggcgTCGCCAGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAGAGCGCCTTGCGCACGCCGGTTCAGa                    <  7:223093/134‑1 (MQ=255)
                                                                                                                                       tatgtggcgTCGCCAGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGa                    >  8:223093/7‑139 (MQ=255)
                                                                                                                                         caACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAAc        >  4:228462/1‑149 (MQ=255)
                                                                                                                                           aCGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACgg      >  2:173181/1‑149 (MQ=255)
                                                                                                                                            cgcgTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACggg     <  6:286881/149‑1 (MQ=255)
                                                                                                                                               gTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCt   >  8:269533/1‑148 (MQ=255)
                                                                                                                                               gTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCTg  >  7:204378/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GAGGTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAGCCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAG‑GCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCTG  >  NC_002947/3759836‑3760127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑