Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,779,129	A→T	6.3%	S112T (TCT→ACT)	nikR ←	nickel‑responsive regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,779,129	0	A	T	6.3%	179.0 / 2.4	63	S112T (TCT→ACT)	nikR	nickel‑responsive regulator
Reads supporting (aligned to +/- strand):  ref base A (32/27);  new base T (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

CGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTG  >  NC_002947/3778985‑3779274                                                                                                                                                 |                                                                                                                                                   cGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAAc                                                                                                                                                <  2:305173/149‑1 (MQ=255)  gTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACt                                                                                                                                               >  7:220853/1‑149 (MQ=255)         ttCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATAACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGATACAACTCGCCAAt                                                                                                                                        >  8:89402/1‑149 (MQ=255)              aGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGTTGTCTCGCCAAttga                                                                                                                                     <  8:336981/147‑3 (MQ=255)                  aCGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGGAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCt                                                                                                                               >  1:21273/1‑149 (MQ=255)                  aCGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCt                                                                                                                               >  5:184259/1‑149 (MQ=255)                   cGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGcc                                                                                                                                  <  4:86018/145‑1 (MQ=255)                   cGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGcc                                                                                                                                  >  3:86018/1‑145 (MQ=255)                       ccTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGc                                                                                                                          <  8:218421/149‑1 (MQ=255)                        cTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGcc                                                                                                                         <  3:352603/149‑1 (MQ=255)                              ccGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTgcag                                                                                                                   >  8:304750/1‑149 (MQ=255)                                    gcgTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCga                                                                                                             <  2:21273/149‑1 (MQ=255)                                       tCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACt                                                                                                                                               >  7:144292/1‑112 (MQ=255)                                       tCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACt                                                                                                                                               <  8:144292/112‑1 (MQ=255)                                         aCGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCt                                                                                                        >  7:213460/1‑149 (MQ=255)                                              cAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAgg                                                                                                   >  4:196909/1‑149 (MQ=255)                                                aGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAgg                                                                                                   >  8:316716/1‑147 (MQ=255)                                                  tGAGGCAACGCCTCAGCGCACCATCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGt                                                                                               <  8:293870/149‑1 (MQ=255)                                                      gCAACGCCTCAGCGCACCTTCCGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTCGCGAGACAACTCGCCAATTCGGCCGCATTCGACCTGCAGCACCGAGACCTCCAGGCACTGGcc                                                                                           >  7:289563/1‑149 (MQ=255)                                                       cAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCg                                                                                          >  6:315227/1‑149 (MQ=255)                                                        aaCGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCgg                                                                                         >  7:266575/1‑149 (MQ=255)                                                             cTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATc                                                                                     >  8:10812/1‑148 (MQ=255)                                                                  cgcACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTg                                                                               >  7:70860/1‑149 (MQ=255)                                                                        ttCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACAt                                                                          >  5:145282/1‑148 (MQ=255)                                                                          cGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACAt                                                                          <  6:145282/146‑1 (MQ=255)                                                                          cGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCACAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGc                                                                        <  1:293464/148‑1 (MQ=255)                                                                             cTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGc                                                                        <  5:353214/145‑1 (MQ=255)                                                                             cTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGc                                                                        >  6:353214/1‑145 (MQ=255)                                                                               gCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTg                                                                  <  7:304750/149‑1 (MQ=255)                                                                                   cGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑c                                                             <  5:315227/149‑1 (MQ=255)                                                                                       gCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGt                                                         <  6:184259/149‑1 (MQ=255)                                                                                             aTCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGt                                                         >  1:337528/1‑143 (MQ=255)                                                                                                acctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCgtg                                                <  8:266575/149‑1 (MQ=255)                                                                                                  ctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCgtggt                                              <  7:316716/149‑1 (MQ=255)                                                                                                  ctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCgtggt                                              >  5:41609/1‑149 (MQ=255)                                                                                                  ctgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCgtggt                                              >  4:203319/1‑149 (MQ=255)                                                                                                   tgcacctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCgtggtg                                             >  2:131710/1‑149 (MQ=255)                                                                                                      acctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAg                                                                      <  6:113704/122‑1 (MQ=255)                                                                                                      acctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGc                                          >  2:269046/1‑149 (MQ=255)                                                                                                        ctgGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGt                                        <  8:213460/149‑1 (MQ=255)                                                                                                           gCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCa                                     <  1:131710/149‑1 (MQ=255)                                                                                                              aTGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAggg                                  >  1:117678/1‑149 (MQ=255)                                                                                                               tGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGt                                 <  6:41609/149‑1 (MQ=255)                                                                                                                 ttCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTcc                                                                                                      <  4:135362/79‑1 (MQ=255)                                                                                                                 ttCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTcc                                                                                                      >  3:135362/1‑79 (MQ=255)                                                                                                                   ccACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTgg                             >  3:305213/1‑149 (MQ=255)                                                                                                                    cACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGt                            >  8:175178/1‑149 (MQ=255)                                                                                                                    cACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGt                            >  1:35510/1‑149 (MQ=255)                                                                                                                       gcctcGCTCAACCATCACTTGGCGAGACAACTCTCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCATGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGa                         <  8:289563/149‑1 (MQ=255)                                                                                                                       gccgcGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGa                         <  3:196909/149‑1 (MQ=255)                                                                                                                        ccgcGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAg                        <  4:305213/149‑1 (MQ=255)                                                                                                                          gcgcTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGcc                      <  8:220853/149‑1 (MQ=255)                                                                                                                                aaCCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGa                                                     <  2:160226/112‑1 (MQ=255)                                                                                                                                aaCCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGa                                                     >  1:160226/1‑112 (MQ=255)                                                                                                                                aaCCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTgg                >  1:95548/1‑149 (MQ=255)                                                                                                                                aaCCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTgg                >  7:19779/1‑149 (MQ=255)                                                                                                                                  ggccgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAgg                                   >  2:182637/6‑128 (MQ=255)                                                                                                                                  ggccgaaTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAgg                                   <  1:182637/123‑1 (MQ=255)                                                                                                                                    aTCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAg            >  4:73479/1‑149 (MQ=255)                                                                                                                                       aaTTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGATCCGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGtt          <  1:269046/149‑1 (MQ=255)                                                                                                                                       aaTTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTc         >  3:29664/1‑149 (MQ=255)                                                                                                                                        aTTGGCGAGTTGTCTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTcg        >  7:306028/1‑149 (MQ=255)                                                                                                                                         ttGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTcgc       <  2:117678/149‑1 (MQ=255)                                                                                                                                              gagaCAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTg  >  3:36536/1‑149 (MQ=255)                                                                                                                                                 aCAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGt           >  3:81027/1‑137 (MQ=255)                                                                                                                                                 aCAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGt           <  4:81027/137‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CGTTGGACTTCTGAGTAACGGCCCTTGGGCCGATCGCGTCACGAACAAGTGAGGCAACGCCTCAGCGCACCTTCGGCTGCCCCGGAGCCGGGATCACCTGCACCTGGCCATGTTCCACGCCGCGCTCAACCATCAATTGGCGAGACAACTCGCCAATTCGGCCGCTTTCGCCCTGCAGCACCGAGACCTCCAGGCAGTGGCCGGCATCCAGGTGCACATGCAGGGTGGAT‑CGGGTCAGATCGTGGTGGGCGTGCAGGGTCTGGTTGAGCCGTCTGGGCAGTTCGCGGGTG  >  NC_002947/3778985‑3779274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑