Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 283,151 T→A 6.2% I201N (ATC→AAC)  betT‑II → choline/carnitine/betaine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947283,1510TA 6.2% 198.5 / 3.2 65I201N (ATC→AAC) betT‑IIcholine/carnitine/betaine transporter
Reads supporting (aligned to +/- strand):  ref base T (32/29);  new base A (2/2);  total (34/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGGCGCGGCTGTG  >  NC_002947/283004‑283293
                                                                                                                                                   |                                                                                                                                              
gCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGcat                                                                                                                                                >  8:11462/1‑148 (MQ=255)
       aGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCa                                                                                                                                        <  7:233589/149‑1 (MQ=255)
       aGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCa                                                                                                                                        <  2:61935/149‑1 (MQ=255)
              gCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGtt                                                                                                                                 <  6:313712/149‑1 (MQ=255)
              gCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGtt                                                                                                                                 >  2:274321/1‑149 (MQ=255)
                cTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCg                                                                                                                               <  4:1324/149‑1 (MQ=255)
                    gtcgGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCt                                                                                                                           >  4:150804/1‑149 (MQ=255)
                       ggcatggcatTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTgg                                                                                                                         <  5:84414/148‑1 (MQ=255)
                           tggcatTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGTCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGcc                                                                                                                    <  1:274321/149‑1 (MQ=255)
                            ggcatTGGCCTATTTTGCCTACCGGCACTACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGcc                                                                                                                    >  1:203808/1‑148 (MQ=255)
                                   gCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATggg                                                                                                            >  1:238992/1‑149 (MQ=255)
                                   gCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATgg                                                                                                             <  6:26877/148‑1 (MQ=255)
                                    ccTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGc                                                                                                           >  8:245676/1‑149 (MQ=255)
                                          ttGCCTACCTGCACAACCTACCGCTGGAGCTGCGTTCGGCTCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTgg                                                                                                      <  1:141883/148‑1 (MQ=255)
                                           tGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTggg                                                                                                     <  7:11462/148‑1 (MQ=255)
                                              cTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGtgc                                                                                                 <  2:238992/149‑1 (MQ=255)
                                                  cGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCGTGGGGGGGGTGTTGGGGTTTGGGGtgggggt                                                                                             >  2:142963/1‑144 (MQ=255)
                                                            tACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCAACATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGcc                                                                                   >  4:168950/1‑149 (MQ=255)
                                                            tACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCAACATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGcc                                                                                   >  4:163414/1‑149 (MQ=255)
                                                              ccGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCg                                                                                  <  2:203808/148‑1 (MQ=255)
                                                               cGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGt                                                                                <  7:245676/149‑1 (MQ=255)
                                                                      ccTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGtg                                                                                                  >  6:102739/1‑124 (MQ=255)
                                                                      ccTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTg                                                                             >  4:272075/1‑145 (MQ=255)
                                                                      ccTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTg                                                                             <  3:272075/145‑1 (MQ=255)
                                                                                gcgcTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCgg                                                               >  3:257081/1‑149 (MQ=255)
                                                                                     gTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTAt                                                            >  3:6292/1‑147 (MQ=255)
                                                                                      tACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCCGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATca                                                          <  1:142963/148‑1 (MQ=255)
                                                                                         ccGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACg                                                                                     >  5:49204/1‑118 (MQ=255)
                                                                                         ccGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATcagcca                                                      >  3:110015/1‑149 (MQ=255)
                                                                                         ccGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGAATCTGAACg                                                                                     <  6:49204/118‑1 (MQ=255)
                                                                                               aTTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATcagccacagcca                                                <  3:150804/149‑1 (MQ=255)
                                                                                                   gCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTAc                                                                       >  7:299740/1‑122 (MQ=255)
                                                                                                   gCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTAc                                                                       <  8:299740/122‑1 (MQ=255)
                                                                                                   gCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATca                                                          <  6:83864/135‑1 (MQ=255)
                                                                                                   gCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATca                                                          >  5:83864/1‑135 (MQ=255)
                                                                                                   gCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTgg                                            >  7:250822/1‑149 (MQ=255)
                                                                                                    cAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTg                                                                                                       >  7:75237/1‑89 (MQ=255)
                                                                                                    cAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTg                                                                                                       <  8:75237/89‑1 (MQ=255)
                                                                                                       gcgcATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATca                                                          <  2:260985/131‑1 (MQ=255)
                                                                                                       gcgcATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATca                                                          >  1:260985/1‑131 (MQ=255)
                                                                                                         gcATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTgg                                                                            >  4:200371/1‑111 (MQ=255)
                                                                                                         gcATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTgg                                                                            <  3:200371/111‑1 (MQ=255)
                                                                                                          cATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAgg                                     <  4:257081/149‑1 (MQ=255)
                                                                                                                  gCCCGCTCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGCACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAgg                                     >  1:278779/1‑141 (MQ=255)
                                                                                                                  gCCCGCTCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAgg                                     <  2:278779/141‑1 (MQ=255)
                                                                                                                  gCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCt                              >  5:35396/1‑148 (MQ=255)
                                                                                                                    ccGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCtcat                           >  6:10953/1‑149 (MQ=255)
                                                                                                                     cGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTgg                                            <  4:249902/131‑1 (MQ=255)
                                                                                                                     cGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTgg                                            >  3:249902/1‑131 (MQ=255)
                                                                                                                     cGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCtcat                           >  2:264143/1‑148 (MQ=255)
                                                                                                                      gATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGt                                          >  4:263968/1‑132 (MQ=255)
                                                                                                                      gATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGt                                          <  3:263968/132‑1 (MQ=255)
                                                                                                                       aTCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCAc                        >  5:361398/1‑149 (MQ=255)
                                                                                                                        tCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCAcc                       <  4:6292/149‑1 (MQ=255)
                                                                                                                          ggCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCt                     >  7:197486/1‑149 (MQ=255)
                                                                                                                                   gTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCAACAGCCCGAGGGTGCAGGTGATCCTCATCACCCTGATGATggg            >  4:216198/1‑149 (MQ=255)
                                                                                                                                      gATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTg                                         >  7:13885/1‑117 (MQ=255)
                                                                                                                                      gATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTg                                         <  8:13885/117‑1 (MQ=255)
                                                                                                                                      gATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGgcgc         >  7:18342/1‑149 (MQ=255)
                                                                                                                                         ggCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGGCGCgg       <  4:110015/148‑1 (MQ=255)
                                                                                                                                            ttCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGt                                          <  8:163285/110‑1 (MQ=255)
                                                                                                                                            ttCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGt                                          >  7:163285/1‑110 (MQ=255)
                                                                                                                                            ttCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGGCGCGGCtgt   >  6:139411/1‑149 (MQ=255)
                                                                                                                                            ttCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGGATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGGCGCGGCtgt   >  1:187490/1‑149 (MQ=255)
                                                                                                                                              cGGCAACATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGGCGCGGCtgtg  <  3:163414/148‑1 (MQ=255)
                                                                                                                                              cGGCAACATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCAGCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGTTGCAGGTGATCCTCATCACCCTGATGATGGGCGCGGCtgtg  <  3:168950/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GCTGGGGAGTGTTCGCCTTCGTCGGCATGGCATTGGCCTATTTTGCCTACCGGCACAACCTACCGCTGGCCCTGCGTTCGGCGCTGTACCCGCTGATTGGCAAGCGCATCAACGGCCCGATCGGCTATGCGGTGGATGGCTTCGGCATCATCGCCACGGTGTTCGGCCTGGGCGCCGACATGGGCTTTGGGGTGCTGCATCTGAACGCCGGTCTGGACTACCTGTTCGGTATCAGCCACAGCCAGTGGGTGCAGGTGATCCTCATCACCCTGATGATGGGCGCGGCTGTG  >  NC_002947/283004‑283293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑