Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,879,680 C→A 7.1% P505P (CCC→CCA)  mexF → multidrug RND transporter MexF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,879,6800CA 7.1% 177.2 / 4.2 56P505P (CCC→CCA) mexFmultidrug RND transporter MexF
Reads supporting (aligned to +/- strand):  ref base C (28/24);  new base A (2/2);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CTGCAGCGTTCATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGCCCTGTTCGTGTATG  >  NC_002947/3879535‑3879828
                                                                                                                                                 |                                                                                                                                                    
cTGCAGCGTTCATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAg                                                                                                                                                   >  3:252134/1‑149 (MQ=255)
     gCGTTCATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCg                                                                                                                                              >  7:308150/1‑149 (MQ=255)
       gTTCATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAg                                                                                                                                                   >  8:189837/1‑142 (MQ=255)
       gTTCATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAg                                                                                                                                                   <  7:189837/142‑1 (MQ=255)
          cATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTtc                                                                                                                                          <  3:22931/148‑1 (MQ=255)
             ttCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTcgc                                                                                                                                      >  2:261799/1‑149 (MQ=255)
                        ccGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGa                                                                                                                            <  2:207133/148‑1 (MQ=255)
                                ttCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAgctgct                                                                                                                   >  4:238601/1‑149 (MQ=255)
                                    aCAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTggg                                                                                                                >  7:217578/1‑148 (MQ=255)
                                    aCAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTggg                                                                                                                >  6:289313/1‑148 (MQ=255)
                                        gCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgct                                                                                                           >  6:228564/1‑149 (MQ=255)
                                        gCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgct                                                                                                           <  4:252134/149‑1 (MQ=255)
                                           gTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgctggc                                                                                                        <  1:162745/149‑1 (MQ=255)
                                           gTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgctggc                                                                                                        <  1:157342/149‑1 (MQ=255)
                                           gTTCGCCCTGACCATCGCAATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgctggc                                                                                                        >  4:108458/1‑149 (MQ=255)
                                            ttCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCCCAGCAgcttgtc                                                                                                       <  1:261799/149‑5 (MQ=255)
                                                cccTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGGTGCTgg                                                                                                                 >  2:231913/1‑135 (MQ=255)
                                                cccTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGGTGCTgg                                                                                                                 <  1:231913/135‑1 (MQ=255)
                                                cccTGACCATCGCCACCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTtc                                                                                                   >  3:75630/1‑149 (MQ=255)
                                                     aCCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTGTcccc                                                                                              <  3:108458/149‑1 (MQ=255)
                                                        aTCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCttt                                                                                           >  3:344718/1‑149 (MQ=255)
                                                        aTCGCCACCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCttt                                                                                           <  4:75630/149‑1 (MQ=255)
                                                            ccATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAAc                                                                                       >  5:10224/1‑149 (MQ=255)
                                                               tctcGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCgg                                                                                    >  2:138353/1‑149 (MQ=255)
                                                                  cGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGttc                                                                                 >  6:163407/1‑149 (MQ=255)
                                                                     ccGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCAAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGttcttc                                                                              >  3:316385/1‑149 (MQ=255)
                                                                     ccGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCAAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGttcttc                                                                              >  3:311327/1‑149 (MQ=255)
                                                                       gTCATTTCGGCGTTCAACTCGCTGGCCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGa                                                                            >  2:156039/1‑149 (MQ=255)
                                                                           tttCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCgg                                                                        <  5:228564/149‑1 (MQ=255)
                                                                              cGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgctgg                                                                                                         >  8:256366/1‑113 (MQ=255)
                                                                              cGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgctgg                                                                                                         <  7:256366/113‑1 (MQ=255)
                                                                              cGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGgc                                                                      >  5:342779/1‑148 (MQ=255)
                                                                                  gTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAAcc                                                                                      <  4:233207/128‑1 (MQ=255)
                                                                                  gTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAAcc                                                                                      >  3:233207/1‑128 (MQ=255)
                                                                                   ttCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGgccagcca                                                                >  4:331587/1‑149 (MQ=255)
                                                                                        cTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCt                                                           <  6:10224/149‑1 (MQ=255)
                                                                                              gACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCg                                                     >  2:56787/1‑149 (MQ=255)
                                                                                               aCCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCgg                                                    >  8:214037/1‑149 (MQ=255)
                                                                                               aCCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCg                                                     <  5:163407/148‑1 (MQ=255)
                                                                                                   tGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGc                                                >  3:166858/1‑149 (MQ=255)
                                                                                                           gCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCg                                        <  4:344718/149‑1 (MQ=255)
                                                                                                            ccTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCgg                                       >  4:104411/1‑149 (MQ=255)
                                                                                                                  ccGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATc                                 <  3:238601/149‑1 (MQ=255)
                                                                                                                  acgcgGGGCTGCTCAAGGATCATCACGCCCCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATc                                 <  8:308150/148‑1 (MQ=255)
                                                                                                                     cgGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGc                              <  5:289313/149‑1 (MQ=255)
                                                                                                                     cgGTGCTGCTCAAGGATCATCACGCACCAAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGc                              <  4:316385/149‑1 (MQ=255)
                                                                                                                     cgGTGCTGCTCAAGGATCATCACGCACCAAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGc                              <  4:311327/149‑1 (MQ=255)
                                                                                                                             ctCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCgg                      <  1:56787/149‑1 (MQ=255)
                                                                                                                              tCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgct                                                                                                           <  4:110012/63‑1 (MQ=255)
                                                                                                                              tCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAgct                                                                                                           >  3:110012/1‑63 (MQ=255)
                                                                                                                                    atcatcACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGcc               >  8:65403/1‑149 (MQ=255)
                                                                                                                                      catcaCGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGCCCt             >  5:211119/1‑149 (MQ=255)
                                                                                                                                       atcaCGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGCCCTg            <  3:104411/149‑1 (MQ=255)
                                                                                                                                        tcaCGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGCCCTGt           <  7:65403/149‑1 (MQ=255)
                                                                                                                                          aCGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGt                                                   <  8:176450/107‑1 (MQ=255)
                                                                                                                                          aCGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGt                                                   >  7:176450/1‑107 (MQ=255)
                                                                                                                                                 cAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGCCCTGTTCGTGTATg  >  3:305547/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTGCAGCGTTCATTTCCGGCCTTACCGGGCAGTTCTACAAGCAGTTCGCCCTGACCATCGCCATCTCGACCGTCATTTCGGCGTTCAACTCGCTGACCCTGTCGCCAGCCTTGGCCGCGGTGCTGCTCAAGGATCATCACGCACCCAAGGACCGTTTCTCGCGGTTCCTCGACAAGCTGCTGGGCAGCTGGCTGTTCTCCCCTTTCAACCGGTTCTTCGACCGGGCCAGCCACAGCTACGTCGGTGGCGTGCGCCGGGTCATCCGCTCCAGCGGCATCGCCCTGTTCGTGTATG  >  NC_002947/3879535‑3879828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑