Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,892,916 C→A 6.7% L94M (CTG→ATG)  rarD → chloramphenicol‑sensitive protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,892,9160CA 6.7% 188.5 / 4.0 60L94M (CTG→ATG) rarDchloramphenicol‑sensitive protein
Reads supporting (aligned to +/- strand):  ref base C (24/32);  new base A (2/2);  total (26/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

CTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGAGC  >  NC_002947/3892774‑3893063
                                                                                                                                                  |                                                                                                                                                   
cTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATg                                                                                                                                               >  7:151229/1‑149 (MQ=255)
   gTTCATGCTTCGCTCGAAAGCCTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGTCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATgg                                                                                                                                              <  5:52743/147‑1 (MQ=255)
       aTGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAg                                                                                                                                        >  2:205101/1‑149 (MQ=255)
          cTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGTCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCATGCAGGGgcgcccag                                                                                                                                      <  8:152468/148‑5 (MQ=255)
          cTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGcc                                                                                                                                      <  2:346548/148‑1 (MQ=255)
             gTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTgg                                                                                                                                   <  1:196720/148‑1 (MQ=255)
             gTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTg                                                                                                                                    <  2:336212/147‑1 (MQ=255)
             gTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTg                                                                                                                                    <  2:160496/147‑1 (MQ=255)
              tctcGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGaa                                                                                                                                 <  7:24594/149‑1 (MQ=255)
              tctcGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGaa                                                                                                                                 <  4:347908/149‑1 (MQ=255)
              tctcGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGaa                                                                                                                                 <  8:151229/149‑1 (MQ=255)
              tctcGAAAGACTGGAAGCGGGTGGGCGAACTGATGGCCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGa                                                                                                                                  <  7:339295/148‑1 (MQ=255)
                 ccaaaGACTGGAAGCGGGGGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGGCCTGGCTGATGGGGGTGCAACTGCTGGCTGTTTCTCTGGGCGCCCCTGCa                                                                                                                                                 <  1:186710/129‑1 (MQ=255)
                 cGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCa                                                                                                                                                 >  2:186710/1‑130 (MQ=255)
                 cGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCAt                                                                                                                                                <  3:3115/131‑1 (MQ=255)
                 cGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCAt                                                                                                                                                >  4:3115/1‑131 (MQ=255)
                      gACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGAt                                                                                                                         >  4:339986/1‑149 (MQ=255)
                        cTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATgg                                                                                                                       <  1:205101/149‑1 (MQ=255)
                            aaGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTa                                                                                                                   >  1:67020/1‑149 (MQ=255)
                                gggTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTc                                                                                                               <  3:76953/149‑1 (MQ=255)
                                    gggCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCctgc                                                                                                           >  3:300826/1‑149 (MQ=255)
                                     ggCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTGCCCCCAGCATTGGTCGGCACGTCCTGTCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCct                                                                                                             <  1:119757/149‑1 (MQ=255)
                                     ggCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCctgct                                                                                                          >  7:122645/1‑149 (MQ=255)
                                      gCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGAt                                                                                                                         <  5:211995/133‑1 (MQ=255)
                                      gCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGAt                                                                                                                         >  6:211995/1‑133 (MQ=255)
                                            tGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATgg                                                                                                                       >  4:2586/1‑129 (MQ=255)
                                            tGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATgg                                                                                                                       <  3:2586/129‑1 (MQ=255)
                                                 ggCCGGGTATAACGCACGCAGGGGCTGGTT‑‑CTCGGCA‑TGGTCGGCACGTCCTGCCTTATGGGGGTGCAACTG‑TGGCTTTTTCTCTGGGCTCCCCTGCATGGGCGCAGACTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCAt                                                                                              <  4:204012/149‑1 (MQ=255)
                                                 ggCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCa                                                                                               <  2:67020/148‑1 (MQ=255)
                                                  gCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATg                                                                                             >  8:84372/1‑149 (MQ=255)
                                                        tAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCt                                                                                        >  4:1313/1‑148 (MQ=255)
                                                          aaaCGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGa                                                                                      <  4:300826/148‑1 (MQ=255)
                                                              gcacgcCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCggg                                                                                 >  7:212890/1‑149 (MQ=255)
                                                                  gcCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAgt                                                                                                                               >  7:268106/1‑99 (MQ=255)
                                                                  gcCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAgt                                                                                                                               <  8:268106/99‑1 (MQ=255)
                                                                   ccGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCt                                                                            <  5:67217/149‑1 (MQ=255)
                                                                      gTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGt                                                                         <  3:1313/149‑1 (MQ=255)
                                                                       tGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGt                                                                                                                     <  5:204423/104‑1 (MQ=255)
                                                                       tGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGt                                                                                                                     <  5:199389/104‑1 (MQ=255)
                                                                       tGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGt                                                                                                                     >  6:204423/1‑104 (MQ=255)
                                                                       tGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGt                                                                                                                     >  6:199389/1‑104 (MQ=255)
                                                                          tGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTatat                                                                     >  6:103120/1‑149 (MQ=255)
                                                                              g‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCGACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGc                                                                  <  4:115846/148‑1 (MQ=255)
                                                                              g‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTg                                                                                                   >  5:71243/1‑115 (MQ=255)
                                                                              g‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTg                                                                                                   <  6:71243/115‑1 (MQ=255)
                                                                                    ggCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCg                                                             >  5:23542/1‑149 (MQ=255)
                                                                                     gCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCgg                                                            <  8:212890/149‑1 (MQ=255)
                                                                                      cA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGc                                                           >  6:261816/1‑149 (MQ=255)
                                                                                                 aCGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCt                                                 <  5:261816/149‑1 (MQ=255)
                                                                                                     ccTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTg                                                >  6:287916/1‑146 (MQ=255)
                                                                                                         gCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACg                                          <  8:122645/148‑1 (MQ=255)
                                                                                                                             tgGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATg                                                                    >  2:23140/1‑103 (MQ=255)
                                                                                                                              ggCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCa                     <  7:84372/149‑1 (MQ=255)
                                                                                                                                 tGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTg                  >  2:155854/1‑149 (MQ=255)
                                                                                                                                          gggCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTg                                 <  6:325281/125‑1 (MQ=255)
                                                                                                                                          gggCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTg                                 >  5:325281/1‑125 (MQ=255)
                                                                                                                                          ctgcgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGc         >  2:108573/3‑149 (MQ=255)
                                                                                                                                           ggCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACTGTAGCCGTGGCCTGCGCCGCACTGGGCGTgg           <  5:103120/146‑1 (MQ=255)
                                                                                                                                             cgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTgg                                <  2:230672/123‑1 (MQ=255)
                                                                                                                                             cgcCCATGCAGGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTgg                                >  1:230672/1‑123 (MQ=255)
                                                                                                                                                 ccTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGAGc  >  8:228932/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTTGTTCATGCTTGTCTCGAAAGACTGGAAGCGGGTGGGCGAACTGATGGGCCGGGTAAAACGCACGCCGGTGCTGTTG‑‑CTCGGCA‑TGGTCGGCACGTCCTGGCTGATGGGGGTGCAACTG‑TGGCTGTTTCTCTGGGCGCCCCTGCATGGGCGCAGCCTGGAAGTGTCGATGGGTTACTTCCTGCTGCCACTGGCCATGGTCCTGACCGGGCGGCTGGTATATGGCGAGCGGCTGTCACGCCTGCAGACGGTAGCCGTGGCCTGCGCCGCACTGGGCGTGGGCCACGAGC  >  NC_002947/3892774‑3893063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑