| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 25,224 | T→A | 6.3% | V177D (GTC→GAC) | PP_0020 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 25,224 | 0 | T | A | 6.3% | 189.7 / 3.1 | 63 | V177D (GTC→GAC) | PP_0020 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (28/31); new base A (2/2); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01 | |||||||||||
GTAATCACGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGACCAAACGAGAGCACGCCAGGCGCGCAAAT > NC_002947/25079‑25369 | gTAATCACGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTcga < 6:234310/149‑1 (MQ=255)gTAATCACGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTcga > 5:3344/1‑149 (MQ=255) tAATCACGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTcgac < 4:211297/149‑1 (MQ=255) aCGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGTGCATCGCCGTGCTTCTCGACGAtt > 4:316652/1‑148 (MQ=255) aCGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGAttt > 6:95277/1‑149 (MQ=255) gCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTtcc > 7:212605/1‑149 (MQ=255) aagaaAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCAt > 8:335035/1‑146 (MQ=255) aagaaAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAg < 8:212605/149‑1 (MQ=255) gaaAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGaa < 5:141475/149‑1 (MQ=255) aaCGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACAGTATTGCGATGCCGGACGAGTTGGTCAAACGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGaacaa > 5:251200/1‑149 (MQ=255) tCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAAt > 8:98624/1‑149 (MQ=255) tCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAAt > 4:198283/1‑149 (MQ=255) tctcATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGc < 2:170124/148‑1 (MQ=255) tcATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAg > 8:283427/1‑149 (MQ=255) tcATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATATCCTCCATCAGAACAACCAATTTTTCGCTAg > 1:139629/1‑149 (MQ=255) tGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTg > 5:299455/1‑149 (MQ=255) gATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTcgacga < 6:2544/108‑1 (MQ=255) gATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTcgacga > 5:2544/1‑108 (MQ=255) gATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTgg < 1:347306/148‑1 (MQ=255) gATCTCCCACCATTACAACCGTATTGTGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAGGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTgg < 4:9405/148‑1 (MQ=255) cccACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAAt < 4:192846/126‑1 (MQ=255) cccACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAAt > 3:192846/1‑126 (MQ=255) ccaccaTTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTcc < 7:335035/148‑1 (MQ=255) caccaTTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCtt < 6:3344/149‑1 (MQ=255) accaTTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCtt > 3:346460/1‑148 (MQ=255) aCAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAAccc < 7:283427/148‑1 (MQ=255) ctaCCTTATTGCGATGCCGGACGTGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCa < 6:251200/147‑1 (MQ=255) gCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCCTCGCCGTGCTTGACGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGGTTCCtt < 5:258828/131‑1 (MQ=255) gCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGACGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCtt > 6:258828/1‑131 (MQ=255) gATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTc < 4:346460/149‑1 (MQ=255) aTGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTcg > 5:18407/1‑149 (MQ=255) tGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTcgc < 7:98624/149‑1 (MQ=255) cGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGAc > 5:206271/1‑149 (MQ=255) ggACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGtt > 5:15742/1‑118 (MQ=255) ggACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGtt < 6:15742/118‑1 (MQ=255) gTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTtcctc < 2:100532/76‑1 (MQ=255) gTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTtcctc > 1:100532/1‑76 (MQ=255) gTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCa > 4:23083/1‑121 (MQ=255) gTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCa < 3:23083/121‑1 (MQ=255) tCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGccc > 4:290914/1‑149 (MQ=255) tCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGccc < 6:206271/149‑1 (MQ=255) aCCGCCTGAAGGCAGACCAAGGGGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTAc < 3:232704/149‑1 (MQ=255) cGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTAc > 5:86130/1‑147 (MQ=255) gCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAg < 5:95277/149‑1 (MQ=255) ccTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTgg > 8:65416/1‑97 (MQ=255) ccTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTgg < 7:65416/97‑1 (MQ=255) ccTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAgg < 3:290914/149‑1 (MQ=255) cTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAggt < 3:198283/149‑1 (MQ=255) atGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGCACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCACCCCCAAGGGCGAGATTCGCGAACCGGCTGAGACCTACGAggt > 3:213203/2‑149 (MQ=255) aGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAAc > 2:56015/1‑100 (MQ=255) aGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAAc < 1:56015/100‑1 (MQ=255) aCCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCt < 7:323988/125‑1 (MQ=255) aCCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCt > 8:323988/1‑125 (MQ=255) cAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGtt > 7:67544/1‑149 (MQ=255) ggTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGttttt < 4:100384/149‑1 (MQ=255) ggTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGttttt < 6:299455/149‑1 (MQ=255) tGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGa < 4:144837/149‑1 (MQ=255) acgacgGCATGAAGGGCATCGCCGTGCTTGACGACGATTTCCTCAATCAGAACAACCAATTTTTCGc < 7:224013/67‑1 (MQ=255) acgacgGCATGAAGGGCATCGCCGTGCTTGACGACGATTTCCTCAATCAGAACAACCAATTTTTCGc > 8:224013/1‑67 (MQ=255) gCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGACCAAACga < 6:18407/149‑1 (MQ=255) gggCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGACCAAACGAGAGCACg > 2:296339/1‑149 (MQ=255) gCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGACCAAACGAGAGCACGCCAGgc < 3:316652/148‑1 (MQ=255) tGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGACCAAACGAGAGCACGCCAGGCGCGCAAAt > 3:156032/1‑148 (MQ=255) | GTAATCACGCTGAGCGAAGAAAACGGTCAGTATTTCTCATACTGGATGTCCCACCATTACAACCGTATTGCGATGCCGGACGAGTTGGTCAACCGCCTGAAGGCAGACCAAGGTGACGACGGCATGAAGGGCATCGCCGTGCTTGTCGACGATTTCCTCCATCAGAACAACCAATTTTTCGCTAGTGCTTGGGTTTCCTTCAACCCCAAGGGCGAGATTCGCGACCCGGCTGAGCCCTACGAGGTGGTGTTCCGGTTTTTGACCAAACGAGAGCACGCCAGGCGCGCAAAT > NC_002947/25079‑25369 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |