Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,909,236 C→T 9.8% S395S (AGC→AGT) ‡ PP_3450 → TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,909,2360CT 9.8% 137.0 / 4.6 51S395S (AGC→AGT) ‡PP_3450TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (23/23);  new base T (2/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

GCCGTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCAT  >  NC_002947/3909088‑3909379
                                                                                                                                                    |                                                                                                                                               
gCCGTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTT‑GGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGcc                                                                                                                                                >  6:292196/1‑149 (MQ=255)
   gTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTT‑GGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGc                                                                                                                                             <  5:292196/149‑1 (MQ=255)
       cgcCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCa                                                                                                                                           <  1:343853/148‑1 (MQ=255)
         ccATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                         >  7:202249/1‑148 (MQ=255)
              cccGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAgcgc                                                                                                                                   >  7:222654/1‑149 (MQ=255)
               ccGACCAACCGGGTGAGGCGGTGGGCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCCACCGTGTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTGCGACACCGCGCAGCCGATCTGGCGCAGCCTGCCAGACAGAGcc                                                                                                                                  >  2:228915/1‑149 (MQ=255)
                  aCCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCTGTCTGGCAGGCTGCGccaga                                                                                                                               <  2:59989/149‑4 (MQ=255)
                    cAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTAc                                                                                                                             <  1:45727/149‑1 (MQ=255)
                    cAACCGGGAGAGGAGGTGGTCTACTACCCGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCAACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCATTGAc                                                                                                                             >  6:65080/1‑149 (MQ=255)
                      aCCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTAcc                                                                                                                            >  5:252252/1‑148 (MQ=255)
                          ggTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACGCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCGTTACCGATaa                                                                                                                       >  4:9659/1‑149 (MQ=255)
                              aGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGc                                                                                                                    <  1:72295/148‑1 (MQ=255)
                                gCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGAAACCGTCTACAGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCg                                                                                                                 >  6:296799/1‑149 (MQ=255)
                                  ggtggtCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGcc                                                                                                               >  2:61071/1‑149 (MQ=255)
                                  ggtggtCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGcc                                                                                                               <  8:222654/149‑1 (MQ=255)
                                         tactacCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGc                                                                                                        >  5:159237/1‑149 (MQ=255)
                                            tacCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGc                                                                                                     >  7:138901/1‑149 (MQ=255)
                                                 ggCTGCCGAGGGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                         <  4:300361/108‑1 (MQ=255)
                                                 ggCTGCCGAGAGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                         >  3:300361/1‑108 (MQ=255)
                                                   cTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCgcggc                                                                                              <  1:61071/149‑1 (MQ=255)
                                                      ccGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGgcgcg                                                                                           >  7:160694/1‑149 (MQ=255)
                                                       cGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGgcgcgc                                                                                          <  8:138901/149‑1 (MQ=255)
                                                        gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                         <  7:80477/149‑1 (MQ=255)
                                                        gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                         >  8:80477/1‑149 (MQ=255)
                                                        gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                         <  6:252252/149‑1 (MQ=255)
                                                            gTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAAt                                                                                     >  7:47261/1‑149 (MQ=255)
                                                                  gCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGc                                                                               <  6:115798/149‑1 (MQ=255)
                                                                            cAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATg                                                                     <  8:202249/149‑1 (MQ=255)
                                                                               ccGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATgca                                                                   <  4:134216/148‑1 (MQ=255)
                                                                               ccGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATg                                                                     >  3:134216/1‑146 (MQ=255)
                                                                                      ccGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCAGCCAGACAGCGCCTATACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGTGCTGAATGCAGGCGACGCCGATGCAGCAcgccg                                                           <  1:228915/149‑1 (MQ=255)
                                                                                            gCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTg                                                     >  6:123364/1‑149 (MQ=255)
                                                                                                  gCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGc                                               <  6:159237/149‑1 (MQ=255)
                                                                                                  gCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGc                                               <  5:296799/149‑1 (MQ=255)
                                                                                                   ctcTCGAAGCGGCCGGCGACTCCGAAACGGCCGAGCCGTTCTGGCGCAGCCTGCCAGACAGCGACTTTACCGATAATGCCCGGCTCACCGCTGCCGGCGGCGCGCTGAATGCAGGCTACGCGGATGCATCAGGCCGCTACTGGGActct                                              <  8:98815/147‑1 (MQ=255)
                                                                                                      tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGa                                                                                       >  1:104385/1‑105 (MQ=255)
                                                                                                      tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGa                                                                                       <  2:104385/105‑1 (MQ=255)
                                                                                                      tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGcc                                           >  3:96099/1‑149 (MQ=255)
                                                                                                        gAAGCGGCCGGCGACTCCGAAACCGCCCAGGCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGc                                                                                                                <  8:250129/78‑1 (MQ=255)
                                                                                                        gAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGc                                                                                                                >  7:250129/1‑78 (MQ=255)
                                                                                                             ggccggcGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACg                                                <  7:200437/137‑1 (MQ=255)
                                                                                                             ggccggcGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACg                                                >  8:200437/1‑137 (MQ=255)
                                                                                                                   cGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAgcg                              >  8:115805/1‑149 (MQ=255)
                                                                                                                       tCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCTCCTCACCGCTGCCCGCGCCGCGCTGAATGCAGGCGACGCCGATGCACCACGCCGCTACTGGGACGCTGCCGCGCACCACAGAGCtga                          >  6:102529/1‑149 (MQ=255)
                                                                                                                        ccGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCtgat                         <  5:123364/149‑1 (MQ=255)
                                                                                                                          gAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCtgctga                       >  3:270817/1‑149 (MQ=255)
                                                                                                                              ccGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgg                   <  8:47261/149‑1 (MQ=255)
                                                                                                                                             ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg         <  3:327865/144‑1 (MQ=255)
                                                                                                                                             ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg         >  4:322537/1‑144 (MQ=255)
                                                                                                                                             ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg         <  3:322537/144‑1 (MQ=255)
                                                                                                                                             ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg         >  4:327865/1‑144 (MQ=255)
                                                                                                                                               cgcAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCTCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCAt  <  5:65080/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GCCGTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCAT  >  NC_002947/3909088‑3909379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑