Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,909,240 C→G 9.8% P397A (CCA→GCA)  PP_3450 → TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,909,2400CG 9.8% 149.8 / 6.4 51P397A (CCA→GCA) PP_3450TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base G (2/3);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

GTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAG  >  NC_002947/3909091‑3909386
                                                                                                                                                     |                                                                                                                                                  
gTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTT‑GGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGc                                                                                                                                                    <  5:292196/149‑1 (MQ=255)
    cgcCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCa                                                                                                                                                  <  1:343853/148‑1 (MQ=255)
      ccATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                                >  7:202249/1‑148 (MQ=255)
           cccGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAgcgc                                                                                                                                          >  7:222654/1‑149 (MQ=255)
            ccGACCAACCGGGTGAGGCGGTGGGCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCCACCGTGTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTGCGACACCGCGCAGCCGATCTGGCGCAGCCTGCCAGACAGAGcc                                                                                                                                         >  2:228915/1‑149 (MQ=255)
               aCCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCTGTCTGGCAGGCTGCGccaga                                                                                                                                      <  2:59989/149‑4 (MQ=255)
                 cAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTAc                                                                                                                                    <  1:45727/149‑1 (MQ=255)
                 cAACCGGGAGAGGAGGTGGTCTACTACCCGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCAACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCATTGAc                                                                                                                                    >  6:65080/1‑149 (MQ=255)
                   aCCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTAcc                                                                                                                                   >  5:252252/1‑148 (MQ=255)
                       ggTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACGCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCGTTACCGATaa                                                                                                                              >  4:9659/1‑149 (MQ=255)
                           aGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGc                                                                                                                           <  1:72295/148‑1 (MQ=255)
                             gCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGAAACCGTCTACAGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCg                                                                                                                        >  6:296799/1‑149 (MQ=255)
                               ggtggtCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGcc                                                                                                                      >  2:61071/1‑149 (MQ=255)
                               ggtggtCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGcc                                                                                                                      <  8:222654/149‑1 (MQ=255)
                                      tactacCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGc                                                                                                               >  5:159237/1‑149 (MQ=255)
                                         tacCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGc                                                                                                            >  7:138901/1‑149 (MQ=255)
                                              ggCTGCCGAGGGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                                <  4:300361/108‑1 (MQ=255)
                                              ggCTGCCGAGAGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                                >  3:300361/1‑108 (MQ=255)
                                                cTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCgcggc                                                                                                     <  1:61071/149‑1 (MQ=255)
                                                   ccGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGgcgcg                                                                                                  >  7:160694/1‑149 (MQ=255)
                                                    cGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGgcgcgc                                                                                                 <  8:138901/149‑1 (MQ=255)
                                                     gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                                <  7:80477/149‑1 (MQ=255)
                                                     gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                                <  6:252252/149‑1 (MQ=255)
                                                     gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                                >  8:80477/1‑149 (MQ=255)
                                                         gTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAAt                                                                                            >  7:47261/1‑149 (MQ=255)
                                                               gCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGc                                                                                      <  6:115798/149‑1 (MQ=255)
                                                                         cAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATg                                                                            <  8:202249/149‑1 (MQ=255)
                                                                            ccGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATgca                                                                          <  4:134216/148‑1 (MQ=255)
                                                                            ccGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATg                                                                            >  3:134216/1‑146 (MQ=255)
                                                                                   ccGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCAGCCAGACAGCGCCTATACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGTGCTGAATGCAGGCGACGCCGATGCAGCAcgccg                                                                  <  1:228915/149‑1 (MQ=255)
                                                                                         gCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTg                                                            >  6:123364/1‑149 (MQ=255)
                                                                                               gCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGc                                                      <  6:159237/149‑1 (MQ=255)
                                                                                               gCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGc                                                      <  5:296799/149‑1 (MQ=255)
                                                                                                ctcTCGAAGCGGCCGGCGACTCCGAAACGGCCGAGCCGTTCTGGCGCAGCCTGCCAGACAGCGACTTTACCGATAATGCCCGGCTCACCGCTGCCGGCGGCGCGCTGAATGCAGGCTACGCGGATGCATCAGGCCGCTACTGGGActct                                                     <  8:98815/147‑1 (MQ=255)
                                                                                                   tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGa                                                                                              >  1:104385/1‑105 (MQ=255)
                                                                                                   tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGa                                                                                              <  2:104385/105‑1 (MQ=255)
                                                                                                   tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGcc                                                  >  3:96099/1‑149 (MQ=255)
                                                                                                     gAAGCGGCCGGCGACTCCGAAACCGCCCAGGCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGc                                                                                                                       <  8:250129/78‑1 (MQ=255)
                                                                                                     gAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGc                                                                                                                       >  7:250129/1‑78 (MQ=255)
                                                                                                          ggccggcGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACg                                                       <  7:200437/137‑1 (MQ=255)
                                                                                                          ggccggcGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACg                                                       >  8:200437/1‑137 (MQ=255)
                                                                                                                cGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAgcg                                     >  8:115805/1‑149 (MQ=255)
                                                                                                                    tCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCTCCTCACCGCTGCCCGCGCCGCGCTGAATGCAGGCGACGCCGATGCACCACGCCGCTACTGGGACGCTGCCGCGCACCACAGAGCtga                                 >  6:102529/1‑149 (MQ=255)
                                                                                                                     ccGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCtgat                                <  5:123364/149‑1 (MQ=255)
                                                                                                                       gAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCtgctga                              >  3:270817/1‑149 (MQ=255)
                                                                                                                           ccGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgg                          <  8:47261/149‑1 (MQ=255)
                                                                                                                                          ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                >  4:322537/1‑144 (MQ=255)
                                                                                                                                          ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                <  3:327865/144‑1 (MQ=255)
                                                                                                                                          ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                >  4:327865/1‑144 (MQ=255)
                                                                                                                                          ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                <  3:322537/144‑1 (MQ=255)
                                                                                                                                            cgcAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCTCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCAt         <  5:65080/149‑1 (MQ=255)
                                                                                                                                                   tGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAg  >  5:357636/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTTGCGCCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAG  >  NC_002947/3909091‑3909386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑