Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,909,244 A→G 9.8% D398G (GAC→GGC)  PP_3450 → TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,909,2440AG 9.8% 131.1 / 4.1 51D398G (GAC→GGC) PP_3450TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base G (2/3);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAGCGCC  >  NC_002947/3909097‑3909390
                                                                                                                                                   |                                                                                                                                                  
ccATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                                    >  7:202249/1‑148 (MQ=255)
     cccGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAgcgc                                                                                                                                              >  7:222654/1‑149 (MQ=255)
      ccGACCAACCGGGTGAGGCGGTGGGCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCCACCGTGTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTGCGACACCGCGCAGCCGATCTGGCGCAGCCTGCCAGACAGAGcc                                                                                                                                             >  2:228915/1‑149 (MQ=255)
         aCCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCTGTCTGGCAGGCTGCGccaga                                                                                                                                          <  2:59989/149‑4 (MQ=255)
           cAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTAc                                                                                                                                        <  1:45727/149‑1 (MQ=255)
           cAACCGGGAGAGGAGGTGGTCTACTACCCGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCAACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCATTGAc                                                                                                                                        >  6:65080/1‑149 (MQ=255)
             aCCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTAcc                                                                                                                                       >  5:252252/1‑148 (MQ=255)
                 ggTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACGCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCGTTACCGATaa                                                                                                                                  >  4:9659/1‑149 (MQ=255)
                     aGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGc                                                                                                                               <  1:72295/148‑1 (MQ=255)
                       gCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGAAACCGTCTACAGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCg                                                                                                                            >  6:296799/1‑149 (MQ=255)
                         ggtggtCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGcc                                                                                                                          <  8:222654/149‑1 (MQ=255)
                         ggtggtCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGcc                                                                                                                          >  2:61071/1‑149 (MQ=255)
                                tactacCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGc                                                                                                                   >  5:159237/1‑149 (MQ=255)
                                   tacCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGc                                                                                                                >  7:138901/1‑149 (MQ=255)
                                        ggCTGCCGAGGGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                                    <  4:300361/108‑1 (MQ=255)
                                        ggCTGCCGAGAGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGa                                                                                                                                                    >  3:300361/1‑108 (MQ=255)
                                          cTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCgcggc                                                                                                         <  1:61071/149‑1 (MQ=255)
                                             ccGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGgcgcg                                                                                                      >  7:160694/1‑149 (MQ=255)
                                              cGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGgcgcgc                                                                                                     <  8:138901/149‑1 (MQ=255)
                                               gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                                    <  6:252252/149‑1 (MQ=255)
                                               gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                                    <  7:80477/149‑1 (MQ=255)
                                               gAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCt                                                                                                    >  8:80477/1‑149 (MQ=255)
                                                   gTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAAt                                                                                                >  7:47261/1‑149 (MQ=255)
                                                         gCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGc                                                                                          <  6:115798/149‑1 (MQ=255)
                                                                   cAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATg                                                                                <  8:202249/149‑1 (MQ=255)
                                                                      ccGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATgca                                                                              <  4:134216/148‑1 (MQ=255)
                                                                      ccGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATg                                                                                >  3:134216/1‑146 (MQ=255)
                                                                             ccGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCAGCCAGACAGCGCCTATACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGTGCTGAATGCAGGCGACGCCGATGCAGCAcgccg                                                                      <  1:228915/149‑1 (MQ=255)
                                                                                   gCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTg                                                                >  6:123364/1‑149 (MQ=255)
                                                                                         gCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGc                                                          <  5:296799/149‑1 (MQ=255)
                                                                                         gCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGc                                                          <  6:159237/149‑1 (MQ=255)
                                                                                          ctcTCGAAGCGGCCGGCGACTCCGAAACGGCCGAGCCGTTCTGGCGCAGCCTGCCAGACAGCGACTTTACCGATAATGCCCGGCTCACCGCTGCCGGCGGCGCGCTGAATGCAGGCTACGCGGATGCATCAGGCCGCTACTGGGActct                                                         <  8:98815/147‑1 (MQ=255)
                                                                                             tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGa                                                                                                  >  1:104385/1‑105 (MQ=255)
                                                                                             tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGa                                                                                                  <  2:104385/105‑1 (MQ=255)
                                                                                             tCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGcc                                                      >  3:96099/1‑149 (MQ=255)
                                                                                               gAAGCGGCCGGCGACTCCGAAACCGCCCAGGCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGc                                                                                                                           <  8:250129/78‑1 (MQ=255)
                                                                                               gAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGc                                                                                                                           >  7:250129/1‑78 (MQ=255)
                                                                                                    ggccggcGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACg                                                           <  7:200437/137‑1 (MQ=255)
                                                                                                    ggccggcGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACg                                                           >  8:200437/1‑137 (MQ=255)
                                                                                                          cGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAgcg                                         >  8:115805/1‑149 (MQ=255)
                                                                                                              tCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCTCCTCACCGCTGCCCGCGCCGCGCTGAATGCAGGCGACGCCGATGCACCACGCCGCTACTGGGACGCTGCCGCGCACCACAGAGCtga                                     >  6:102529/1‑149 (MQ=255)
                                                                                                               ccGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCtgat                                    <  5:123364/149‑1 (MQ=255)
                                                                                                                 gAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCtgctga                                  >  3:270817/1‑149 (MQ=255)
                                                                                                                     ccGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgg                              <  8:47261/149‑1 (MQ=255)
                                                                                                                                    ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                    <  3:327865/144‑1 (MQ=255)
                                                                                                                                    ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                    <  3:322537/144‑1 (MQ=255)
                                                                                                                                    ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                    >  4:322537/1‑144 (MQ=255)
                                                                                                                                    ggCGCTGTCTGGCAGGCTGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctgggcg                    >  4:327865/1‑144 (MQ=255)
                                                                                                                                      cgcAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCTCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCAt             <  5:65080/149‑1 (MQ=255)
                                                                                                                                             tGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAg      >  5:357636/1‑149 (MQ=255)
                                                                                                                                                 aGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGCTGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAgcgc   >  8:244187/1‑148 (MQ=255)
                                                                                                                                                 aGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAGCGcc  <  4:96099/149‑1 (MQ=255)
                                                                                                                                                 aGACAGCGCCCTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctg                        >  4:101150/1‑127 (MQ=255)
                                                                                                                                                 aGACAGCGCCCTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGActgggcgctg                        <  3:101150/127‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCATGCCCGACCAACCGGGTGAGGCGGTGGTCTACTACCAGGCTGCCGAGCGTTTGGGCGACCCTGGCAACCGTCTACCGCTGGCCTACGCACTCGAAGCGGCCGGCGACTCCGAAACCGCCCAGCCGATCTGGCGCAGCCTGCCAGACAGCGCCTTTACCGATAATGCCCGCCTCACCGCTGCCCGCGGCGCGCTGAATGCAGGCGACGCCGATGCAGCACGCCGCTACTGGGACGCTGCCGCGCACCAGAGCGCTGATGACTGGGCGCTGGGCGCCGCCATCGCCGAGCGCC  >  NC_002947/3909097‑3909390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑