Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,912,561 T→G 10.9% C303G (TGC→GGC) ‡ PP_3452 → GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,912,5610TG10.9% 170.0 / 11.7 63C303G (TGC→GGC) ‡PP_3452GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base G (4/3);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GGGCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGC  >  NC_002947/3912416‑3912707
                                                                                                                                                 |                                                                                                                                                  
gggCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGc                                                                                                                                                  >  1:242664/1‑148 (MQ=255)
  gCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAg                                                                                                                                               <  5:218419/149‑1 (MQ=255)
  gCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTACGCGATCTTCGTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAg                                                                                                                                               >  5:352130/1‑149 (MQ=255)
   cgacgaAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAgc                                                                                                                                              >  1:351366/1‑149 (MQ=255)
   cgacgaAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAgc                                                                                                                                              <  2:328184/149‑1 (MQ=255)
    gacgaAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAgcg                                                                                                                                             >  7:118798/1‑149 (MQ=255)
       gaAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGt                                                                                                                                          >  8:144515/1‑149 (MQ=255)
              tCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGat                                                                                                                                   >  7:309275/1‑148 (MQ=255)
               cAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAgg                                                                                                                                  >  7:52333/1‑149 (MQ=255)
                     cgTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTg                                                                                                                            <  5:296808/149‑1 (MQ=255)
                         gCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGc                                                                                                                          >  1:328895/1‑147 (MQ=255)
                         gCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGc                                                                                                                          <  2:328895/147‑1 (MQ=255)
                         gCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGa                                                                                                                        <  7:144515/149‑1 (MQ=255)
                          cccTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAg                                                                                                                       <  8:52333/149‑1 (MQ=255)
                           catGCATATCAAGACAAGGCTGCCCGATCAGGCGTATGCCCGGCTGGGTGGCGATCAGTTCGAGATCTTCATCGAAGGGCTGGAGCAGCAAGGGGCCTAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGc                                                                                                                      <  4:188259/147‑1 (MQ=255)
                             tGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCAc                                                                                                                    >  1:244073/1‑149 (MQ=255)
                              gCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCtgt                                                                                                                                                    >  5:9842/1‑116 (MQ=255)
                              gCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCtgt                                                                                                                                                    <  6:9842/116‑1 (MQ=255)
                               cATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCAcc                                                                                                                   <  2:6618/148‑1 (MQ=255)
                                   tCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGt                                                                                                               >  7:189482/1‑148 (MQ=255)
                                   tCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGt                                                                                                               >  8:284939/1‑148 (MQ=255)
                                   tCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTc                                                                                                              >  4:156790/1‑149 (MQ=255)
                                     aaGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAc                                                                                                            <  2:351366/149‑1 (MQ=255)
                                       gACAAGCATGGCCGATGAGGCTTTTGCCCGGCTGGGAGTTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCa                                                                                                             <  6:352130/146‑1 (MQ=255)
                                                    gATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGc                                                                                                                          >  8:273938/1‑120 (MQ=255)
                                                    gATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGc                                                                                                                          <  7:273938/120‑1 (MQ=255)
                                                                  ccGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGAcc                                                                               >  1:35251/1‑149 (MQ=255)
                                                                    ggCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                            >  8:286107/1‑134 (MQ=255)
                                                                    ggCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggcctg                                                                                            <  7:286107/134‑1 (MQ=255)
                                                                         ggAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGgaga                                                                        <  3:156790/149‑1 (MQ=255)
                                                                         ggAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGga                                                                          <  2:244073/147‑1 (MQ=255)
                                                                             gTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGc                                                                    <  2:242664/149‑1 (MQ=255)
                                                                                aaGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCggg                                                                            <  2:176611/138‑1 (MQ=255)
                                                                                aaGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCggg                                                                            >  1:176611/1‑138 (MQ=255)
                                                                                 agagTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTa                                                                >  6:33547/1‑149 (MQ=255)
                                                                                   agTTCGCGATCTTCTTCGAAGGGCTGGATCAGCTAGGGGCCGAGCAGCGAGTGGCCGGGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTac                                                               <  8:118798/148‑1 (MQ=255)
                                                                                   agTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggc                                                                                               >  7:130911/1‑116 (MQ=255)
                                                                                   agTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATtggc                                                                                               <  8:130911/116‑1 (MQ=255)
                                                                                   agTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTacc                                                              <  7:284939/149‑1 (MQ=255)
                                                                                     ttCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTaccac                                                            >  3:140265/1‑149 (MQ=255)
                                                                                        gcgATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACcgc                                                         <  4:25286/149‑1 (MQ=255)
                                                                                          gagcgtcttcGCAGGGCCGGAGCAGCACGGGGCCGACCCGCGAGGGGCCGCGCTGGGCCAGCGCGCGCGCGAGGACGCTGGCGAGCACCCGCCCACCCTCCCCCCTGCCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCgcgc                                                       <  2:35251/144‑1 (MQ=255)
                                                                                             cttcttCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCAACATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTgg                                                    >  6:257386/1‑149 (MQ=255)
                                                                                              ttcttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGt                                                   >  4:160385/1‑149 (MQ=255)
                                                                                                cttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTg                                                     <  5:33547/145‑1 (MQ=255)
                                                                                                 ttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGt                                                   >  8:141227/1‑146 (MQ=255)
                                                                                                 ttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGt                                                   <  7:141227/146‑1 (MQ=255)
                                                                                                 ttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCcg                                                >  3:288031/1‑149 (MQ=255)
                                                                                                 ttcGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCcg                                                >  4:124380/1‑149 (MQ=255)
                                                                                                   cGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTcc                                                 <  6:237521/146‑1 (MQ=255)
                                                                                                   cGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCcgcg                                              >  6:104406/1‑149 (MQ=255)
                                                                                                               tCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCAt                                  >  8:50078/1‑149 (MQ=255)
                                                                                                                 aGCAAGGGGCCGAGCAGCGGGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATta                                >  8:100755/1‑149 (MQ=255)
                                                                                                                                    aGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTg             <  8:189482/149‑1 (MQ=255)
                                                                                                                                    aGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTg             <  4:140265/149‑1 (MQ=255)
                                                                                                                                       gcacgcgCTGGCACAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGc           >  2:43019/4‑148 (MQ=255)
                                                                                                                                        gCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCGTCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATtat                               <  6:329337/127‑1 (MQ=255)
                                                                                                                                        gCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCGTCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATtat                               >  5:329337/1‑127 (MQ=255)
                                                                                                                                        gCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTgg            >  4:302525/1‑146 (MQ=255)
                                                                                                                                        gCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTgg            <  3:302525/146‑1 (MQ=255)
                                                                                                                                        cacgcgCTGGCACAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAg                         <  3:309458/131‑1 (MQ=255)
                                                                                                                                        cacgcgCTGGCACAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAg                         >  4:309458/3‑133 (MQ=255)
                                                                                                                                         acgcgCTGGCACAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAg        >  7:286389/2‑149 (MQ=255)
                                                                                                                                         acgcgCTGGCACAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGACCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTgg            <  6:137818/144‑1 (MQ=255)
                                                                                                                                         acgcgCTGGCACAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGACCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTgg            >  5:137818/2‑145 (MQ=255)
                                                                                                                                               tgtgCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGc  >  3:6824/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGCGACGAAGTGCTCAAGCGCGTGGCCCTGCATATCAAGACAAGCATGGCCGATCAGGCGTTTGCCCGGCTGGGAGGTGAAGAGTTCGCGATCTTCTTCGAAGGGCTGGATCAGCAAGGGGCCGAGCAGCGAGTGGCCGCGCTGTGCCAGCGCGTGCGCGAGGACGCTGGCGAGCACCCGGTCACCATCAGCATTGGCCTGGCCAAGGTCGACCGGGGAGAGAGCCTTACCACCGCGCTGGTCCGCGCCGATCAGGCATTATATGAAGCCAAGCACACTGGCAAGGACCGC  >  NC_002947/3912416‑3912707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑