| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,975,166 | A→C | 11.1% | intergenic (+87/+20) | PP_3504 → / ← PP_3505 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,975,166 | 0 | A | C | 11.1% | 144.2 / 11.6 | 54 | intergenic (+87/+20) | PP_3504/PP_3505 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (25/23); new base C (3/3); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
CGAATTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTGGCGCACGGCATC > NC_002947/3975028‑3975312 | cGAATTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACACTTTGTCGCaa > 4:173268/1‑149 (MQ=255)cGAATTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTc < 8:37381/145‑1 (MQ=255)cGAATTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTc > 7:37381/1‑145 (MQ=255)cGAATTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCaa < 4:330494/149‑1 (MQ=255) aaTTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACACTTTGTCGCaatt < 3:173268/149‑3 (MQ=255) aTTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACACTTTGTCGCaattg < 3:263765/149‑4 (MQ=255) gtgtgAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGc < 4:143686/148‑1 (MQ=255) gtgtgAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGc < 8:300610/148‑1 (MQ=255) tgtgAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGc > 4:352113/1‑147 (MQ=255) tgtgAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCa < 4:115817/149‑1 (MQ=255) gtgAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCa < 3:69969/148‑1 (MQ=255) aaaaGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGtt > 4:71640/1‑148 (MQ=255) aaaaGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGtt < 3:352113/148‑1 (MQ=255) aGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAAt < 3:71640/149‑1 (MQ=255) ccTGCAACCGTGGTCTATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCt < 1:297913/149‑1 (MQ=255) ccTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCt > 3:161296/1‑149 (MQ=255) cTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACACCTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCATCCTGCCAGTTCAAGCCATCACCCt > 6:41551/1‑148 (MQ=255) cTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCt > 5:273666/1‑148 (MQ=255) tGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTg > 3:17077/1‑148 (MQ=255) cGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAAttgtt < 2:30903/149‑1 (MQ=255) ccGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTgg < 6:273666/149‑1 (MQ=255) tAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAAt > 1:262402/1‑147 (MQ=255) caacaaGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCt > 8:62512/1‑149 (MQ=255) aacaaGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCGACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTg > 7:227464/1‑149 (MQ=255) acaaGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGc < 3:13993/149‑1 (MQ=255) aaGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGc > 7:236751/1‑147 (MQ=255) aGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAAcc > 7:50730/1‑82 (MQ=255) aGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAAcc < 8:50730/82‑1 (MQ=255) gtgcgtgcATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTATGCCAGa > 3:195175/1‑149 (MQ=255) tgcgtgcATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCGACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGAc < 8:227464/149‑1 (MQ=255) gTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTgcgcgc < 2:262402/148‑1 (MQ=255) aCTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAgg > 1:44534/1‑149 (MQ=255) gTACAAGTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCAAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCa > 1:193740/1‑149 (MQ=255) tACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTg < 1:301289/131‑1 (MQ=255) tACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTg > 2:301289/1‑131 (MQ=255) aCAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCAc < 3:282121/148‑1 (MQ=255) aaCTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACggg < 4:161296/149‑1 (MQ=255) ttCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAAttgtt < 8:296243/82‑1 (MQ=255) ttCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAAttgtt > 7:296243/1‑82 (MQ=255) tgttgctgttgTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGgc > 1:186249/1‑134 (MQ=255) tgttgctgttgTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGgc < 2:186249/134‑1 (MQ=255) gctgttgTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCa > 1:197415/1‑148 (MQ=255) ctgttgTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCat < 7:62512/148‑1 (MQ=255) ttgtGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATc < 2:197415/148‑1 (MQ=255) ggCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTGATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGAcc > 1:326474/1‑148 (MQ=255) aaCTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACcaac > 1:87174/1‑148 (MQ=255) tggcaggttGCGACACTTTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGt > 2:338879/8‑149 (MQ=255) cccAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTgg > 8:206386/1‑149 (MQ=255) aaTTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTGgcg > 7:86703/1‑148 (MQ=255) gttGCGACACTTTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCTATATCGACca < 2:108753/135‑1 (MQ=255) gttGCGACACTTTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCTATATCGACca > 1:108753/2‑136 (MQ=255) cGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTGGCGCACgg > 2:243354/1‑148 (MQ=255) gACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTGGCGCACGGCa > 3:199481/1‑149 (MQ=255) cAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTGGCGCACGGCATc < 4:17077/149‑1 (MQ=255) | CGAATTGTGTGAAAAGGGCTACGACCTGCAACCGTGGTCGATGCCGAAGTAATATCGGCCAGCACAACAAGTGCGTGCATGGTCACGCACTTGTACAACTTCTCTGTTGCTGTTGTGGCAACTTTCCCAATTGCGACAAAGTGTCGCAACCTGCCAGTTCAATCCATCACCCTGAATTGTTGCAAGTGCTGGTAATCAGCCTGCAATTGTTCTGCCAGACGCTGCGCGCGTCCCAGGCGCACGGGCGCCAGTTCCATATCGACCAACAACGTGGCGCACGGCATC > NC_002947/3975028‑3975312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |