Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,004,143	T→C	10.4%	W37R (TGG→CGG) ‡	PP_3533 →	ornithine cyclodeaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,004,143	0	T	C	10.4%	176.9 / 9.6	69	W37R (TGG→CGG) ‡	PP_3533	ornithine cyclodeaminase
Reads supporting (aligned to +/- strand):  ref base T (29/31);  new base C (3/4);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGGCAAGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTGCATAC  >  NC_002947/4004000‑4004285                                                                                                                                                |                                                                                                                                               tgctgGGCAAGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCTTGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAg                                                                                                                                           >  6:352086/1‑149 (MQ=255)   ctgGGCAAGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAgg                                                                                                                                          <  7:101291/148‑1 (MQ=255)       gCAAGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGttt                                                                                                                                     <  6:270969/149‑1 (MQ=255)       gCAAGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGttt                                                                                                                                     <  5:60354/149‑1 (MQ=255)         aaGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGa                                                                                                                                   >  1:79149/1‑149 (MQ=255)           gcgcACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCCTGCCAGCGTTTGaaa                                                                                                                                 <  4:64531/149‑3 (MQ=255)           gcgcACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCCTGCCAGCGTTTGaaa                                                                                                                                 <  6:155577/149‑3 (MQ=255)            cgcACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACaa                                                                                                                                <  5:352086/149‑1 (MQ=255)            cgcACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACaa                                                                                                                                <  1:192403/149‑1 (MQ=255)               acaATAACAGGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATGTCAAACGCCTGCCAGCGTTTGaaatcgt                                                                                                                             >  4:295649/1‑143 (MQ=255)                caATAACAGGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATGTCAAACGCCTGCCAGCGTTTGaaatcgtc                                                                                                                            <  3:295649/149‑8 (MQ=255)                 aaTAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcg                                                                                                                           >  1:293205/1‑149 (MQ=255)                   tAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGAc                                                                                                                                  <  6:120805/140‑1 (MQ=255)                   tAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGAc                                                                                                                                  >  5:120805/1‑140 (MQ=255)                   tAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcgcg                                                                                                                         >  6:53346/1‑149 (MQ=255)                      cAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcgcgcgc                                                                                                                      >  8:170776/1‑149 (MQ=255)                         ggTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGtt                                                                                                                   <  2:265551/149‑1 (MQ=255)                         ggTGCAAGAGCATGACGTATTTCATAGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGACGTTTATTGGCGAGCTTGCCGCTGCCATGCGGGACGATTTCAAACGAAGGCAGGCGATTGACAAGTCcgcgcgactt                                                                                                                   >  5:225828/1‑145 (MQ=255)                           tGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGCCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTgc                                                                                                                 <  4:113908/149‑1 (MQ=255)                                         gTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAg                                                                                                   >  5:300773/1‑149 (MQ=255)                                            tttCATTGATGTTCCAACCAAGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGGTTATTGGCGAGCTTGCCGCTGCCCTCCGGTACGATTTCACACGAGGGCAGGCGTTTGACAAGTCCGCGAGCGGAGCCAGCCCCTCGGAAGTgg                                                                                                >  8:253846/1‑149 (MQ=255)                                                aTTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGt                                                                                            <  7:337911/149‑1 (MQ=255)                                                 ttGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGt                                                                                            >  7:208690/1‑148 (MQ=255)                                                    aTGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGaa                                                                                                    >  2:269483/1‑137 (MQ=255)                                                    aTGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGaa                                                                                                    <  1:269483/137‑1 (MQ=255)                                                    aTGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGAt                                                                                         <  3:310132/148‑1 (MQ=255)                                                     tGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATc                                                                                        <  8:118363/148‑1 (MQ=255)                                                      gTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCg                                                                                       >  5:1427/1‑148 (MQ=255)                                                       ttCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcgcgcgc                                                                                                                      <  3:42232/116‑1 (MQ=255)                                                       ttCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcgcgcgc                                                                                                                      >  4:42232/1‑116 (MQ=255)                                                       ttCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGaa                                                                                     <  7:170776/149‑1 (MQ=255)                                                       ttCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGaa                                                                                     <  5:53346/149‑1 (MQ=255)                                                        tCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGaa                                                                                     <  8:208690/148‑1 (MQ=255)                                                           aaCCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGa                                                                                 <  4:101601/149‑1 (MQ=255)                                                           aaCCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGa                                                                                 >  3:101601/1‑149 (MQ=255)                                                              cATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGc                                                                              <  2:79149/149‑1 (MQ=255)                                                                tGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCg                                                                            <  1:185395/149‑1 (MQ=255)                                                                   cGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTc                                                                         >  5:331191/1‑149 (MQ=255)                                                                   cGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTc                                                                         <  4:8956/149‑1 (MQ=255)                                                                          ggTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcgcgcgc                                                                                                                      >  5:66024/1‑97 (MQ=255)                                                                          ggTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCcgcgcgc                                                                                                                      <  6:66024/97‑1 (MQ=255)                                                                          ggTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTgccagcca                                                                                                           >  6:100482/1‑108 (MQ=255)                                                                                tGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGcc                                                            >  2:47391/1‑149 (MQ=255)                                                                                   cATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGc                                                          <  6:331191/148‑1 (MQ=255)                                                                                    aTTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTa                                                        >  8:42955/1‑149 (MQ=255)                                                                                    aTTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTa                                                        >  1:280130/1‑149 (MQ=255)                                                                                                        tGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGATCTGATGGCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAAc                                     <  6:225828/148‑1 (MQ=255)                                                                                                          gCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGc                                                          <  8:337852/125‑1 (MQ=255)                                                                                                          gCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGc                                                          >  7:337852/1‑125 (MQ=255)                                                                                                          gCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACgg                                   <  2:280130/148‑1 (MQ=255)                                                                                                            gAGCTTGCCCCTGCCCTCCGGGACGATTACAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTCGGCGTGAACTAACTGATCCCGCTCGCCGACAAAAGCCGCTATGACTTCAAATACGTCACCTGCCa                                >  4:97480/1‑149 (MQ=255)                                                                                                                ttGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCg                            >  5:359728/1‑149 (MQ=255)                                                                                                                ttGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCg                            >  1:29759/1‑149 (MQ=255)                                                                                                                  gCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGc                          >  4:5840/1‑149 (MQ=255)                                                                                                                         cccTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACt                   >  7:121447/1‑149 (MQ=255)                                                                                                                          ccTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTg                  >  2:96168/1‑149 (MQ=255)                                                                                                                             gCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCAc               >  1:48938/1‑149 (MQ=255)                                                                                                                              cGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCAc               <  6:300773/148‑1 (MQ=255)                                                                                                                                 gACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCaa           <  2:293205/149‑1 (MQ=255)                                                                                                                                  aCGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAAc          <  6:1427/149‑1 (MQ=255)                                                                                                                                    gATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCg                            <  2:122754/129‑1 (MQ=255)                                                                                                                                    gATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCg                            >  1:122754/1‑129 (MQ=255)                                                                                                                                     aTTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTg       <  7:42955/149‑1 (MQ=255)                                                                                                                                       ttCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTGCa     >  2:15103/1‑149 (MQ=255)                                                                                                                                       gtCAAACGCCTGCCAGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGc                                                                       <  6:119708/82‑1 (MQ=38)                                                                                                                                       gtCAAACGCCTGCCAGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGc                                                                       >  5:119708/2‑83 (MQ=38)                                                                                                                                       gtCAAACGCCTGCCAGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTGc      >  8:220809/2‑148 (MQ=255)                                                                                                                                        tCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTGCa     <  7:237812/148‑1 (MQ=255)                                                                                                                                          aaaCGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATACCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTGCATAc  >  8:291659/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                               TGCTGGGCAAGCGCACAATAACAAGGTGCAAGAGCATGACGTATTTCATTGATGTTCCAACCATGTCGGACTTGGTGCATGACATTGGCGTAGCGCCGTTTATTGGCGAGCTTGCCGCTGCCCTGCGGGACGATTTCAAACGCTGGCAGGCGTTTGACAAGTCCGCGCGCGTTGCCAGCCACTCGGAAGTGGGCGTGATCGAACTGATGCCGGTCGCCGACAAAAGCCGCTATGCCTTCAAATACGTCAACGGCCACCCGGCCAATACTGCACGCAACCTGCATAC  >  NC_002947/4004000‑4004285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑