| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,021,365 | C→G | 11.1% | *314S (TGA→TCA) | PP_3547 ← | NAD(P)‑binding oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,021,365 | 0 | C | G | 11.1% | 92.7 / 5.6 | 36 | *314S (TGA→TCA) | PP_3547 | NAD(P)‑binding oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (23/9); new base G (2/2); total (25/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
CAAGGTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTCCGG > NC_002947/4021218‑4021510 | cAAGGTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGTGAGCTGCTGGAACCTTGACGGGCGCTCa > 7:119475/1‑149 (MQ=255) tGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAGcgcccgt > 3:137135/1‑148 (MQ=255) gATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGGCGGGCGCTGAGcgcccgtcaag > 5:366959/1‑144 (MQ=255) gATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAGcgcccgtcaa < 3:283405/148‑5 (MQ=255) aTCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTGAGcgcccgtcaag < 4:137135/148‑6 (MQ=255) cGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTc > 3:313188/1‑149 (MQ=255) cGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTc > 7:215316/1‑149 (MQ=255) ggcgTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGtag > 1:10669/1‑146 (MQ=255) ccAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTc > 3:349334/1‑149 (MQ=255) cTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGTAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGc < 1:81205/149‑1 (MQ=255) cccACGCCGTTCTACGTGGACGCAGTGGCATTGGGGGTGCGCTTGCTGCTGTAACTTTGACGGGCGCTCCGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGc < 4:313188/147‑1 (MQ=255) aCCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGc > 1:212310/1‑99 (MQ=255) aCCAAGCCGTTCGAACTGGAAGCACTGGCACAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCa > 1:31199/1‑133 (MQ=255) aaGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCGGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACt > 8:260663/1‑149 (MQ=255) ccGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTcc > 8:273833/1‑149 (MQ=255) gtgcGACCTGCAACCCCTGGCCGCGCGGGTGCGCGAGCTGCTGGACCCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTc < 6:87255/146‑1 (MQ=255) gTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTc > 8:157998/1‑149 (MQ=255) aCTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACg > 3:323523/1‑149 (MQ=255) tGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATTGCCTCGGCCACTTCCTCGGGACGcc > 8:304397/1‑149 (MQ=255) tGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGcc > 1:25662/1‑149 (MQ=255) tGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGcc > 6:159785/1‑149 (MQ=255) gAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCa > 1:24959/1‑115 (MQ=255) gAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGcccc > 8:4426/1‑149 (MQ=255) tGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCACCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATa > 1:255443/1‑84 (MQ=255) agagGGGGTGCGCGAGCTGCTGCAACCTTGCCGGGGGCTCCGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCAt < 2:25662/149‑1 (MQ=255) ggTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCaa > 4:281516/1‑149 (MQ=255) ttCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGc > 1:105852/1‑149 (MQ=255) gcgAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAAc > 3:142694/1‑91 (MQ=255) cgAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAgg > 3:209213/1‑149 (MQ=255) gAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGt < 5:159785/149‑1 (MQ=255) gctgGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGa < 3:281516/148‑1 (MQ=255) tgGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAg > 7:184730/1‑140 (MQ=255) tgGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAg < 8:184730/140‑1 (MQ=255) gAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCCGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCtt < 7:260663/148‑1 (MQ=255) gcgcTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTcc > 6:242200/1‑149 (MQ=255) gcTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTCCgg < 5:242200/149‑1 (MQ=255) | CAAGGTGCTGTTGATCACCGGCTACGATGAGAGTGCGGCGTTCAGCGACGGCCAACCGCTGCAAGGTACGTTGGTGTTGACCAAGCCGTTCGAACTGGAAGCACTGGCAGAGCGGGTTCGCGAGCTGCTGGAACCTTGACGGGCGCTCAGCGCCCGCCGCCCAGGTCGATGAGGCTGCCGGTGGCATAGGAGGCTTTGTCCGAAAGCAACCAGAGGATGGCCTCGGCCACTTCCTCGGGACGCCCCCCTCGGCCCATGGGCAAGCCAGGTTCGAGCTTGCTGACGCGGTCCGG > NC_002947/4021218‑4021510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |