Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,096,650	C→A	10.7%	S86* (TCG→TAG) ‡	PP_5608 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,096,650	0	C	A	10.7%	169.2 / 8.7	63	S86* (TCG→TAG) ‡	PP_5608	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/24);  new base A (2/5);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 2.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGCAGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTT  >  NC_002947/4096503‑4096792                                                                                                                                                    |                                                                                                                                               cgcAGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTcg                                                                                                                                               >  3:296971/1‑149 (MQ=255)       tAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtg                                                                                                                                         <  2:177329/149‑2 (MQ=255)         gccgccGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgtt                                                                                                                                       <  2:63423/149‑4 (MQ=255)         gccgccGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgt                                                                                                                                        <  8:112985/148‑3 (MQ=255)         gccgccGAGGCCCAACACCCCAAGCTCTCCGGCGGAACCCTGTACGCCACGCGCAACAAGCTGCCGGTACGCACATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCaa                                                                                                                                       <  8:91939/149‑1 (MQ=255)                  gCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgttgt                                                                                                                                     <  2:252535/142‑6 (MQ=255)                  gCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCGAGCGGCtgttgt                                                                                                                                     >  1:252535/1‑137 (MQ=255)                    ccAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTc                                                                                                                                         >  3:134083/1‑136 (MQ=255)                    ccAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTc                                                                                                                                         <  4:134083/136‑1 (MQ=255)                      caCAGGCCAATCTTTCCGGGGGAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATc                                                                                                                          <  8:33413/148‑1 (MQ=255)                           gCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAg                                                                                                                       >  5:358874/1‑147 (MQ=255)                                  tttCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTAc                                                                                                              >  6:284028/1‑149 (MQ=255)                                    tCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACtt                                                                                                            >  5:352379/1‑149 (MQ=255)                                       ggggTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTc                                                                                                           >  3:268476/1‑147 (MQ=255)                                        gggTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAg                                                                                                        <  5:95890/149‑1 (MQ=255)                                                 tGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGtt                                                                                               >  5:303982/1‑149 (MQ=255)                                                   tACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTAc                                                                                                              >  8:221167/1‑132 (MQ=255)                                                   tACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTAc                                                                                                              <  7:221167/132‑1 (MQ=255)                                                       ccACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTATAACAGCCGCTCGCGGCTCAAGc                                                                                                                                     <  6:333330/105‑1 (MQ=255)                                                        cACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTa                                                                                                               >  5:38642/1‑126 (MQ=255)                                                         aCGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGt                                                                                                                <  6:51722/124‑1 (MQ=255)                                                         aCGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGt                                                                                                                >  5:51722/1‑124 (MQ=255)                                                              cAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCg                                                                                                 >  7:71686/1‑134 (MQ=255)                                                              cAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCg                                                                                                 <  8:71686/134‑1 (MQ=255)                                                               aagaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTc                                                                                                           >  7:60201/1‑123 (MQ=255)                                                               aagaagCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTc                                                                                                           <  8:60201/123‑1 (MQ=255)                                                                        cAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGAt                                                                         <  5:284028/148‑1 (MQ=255)                                                                        cAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGAt                                                                         >  4:64352/1‑148 (MQ=255)                                                                             aCGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAg                                                                                                        >  8:170422/1‑112 (MQ=255)                                                                             aCGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCAAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAActct                                                                   >  1:83971/1‑149 (MQ=255)                                                                               gCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCtt                                                                  >  7:65355/1‑148 (MQ=255)                                                                               gCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCtt                                                                  >  3:143779/1‑148 (MQ=255)                                                                                cAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTa                                                                <  4:296971/149‑1 (MQ=255)                                                                                   aTTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGAt                                                                         <  4:27449/137‑1 (MQ=255)                                                                                   aTTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGAt                                                                         >  3:27449/1‑137 (MQ=255)                                                                                       cccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCa                                                         >  7:203130/1‑149 (MQ=255)                                                                                       cccAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCa                                                         <  6:303982/149‑1 (MQ=255)                                                                                             cccTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTg                                                   <  6:358874/149‑1 (MQ=255)                                                                                             cccTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTg                                                   <  3:308866/149‑1 (MQ=255)                                                                                                                ccTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATg                                                                                        >  1:266431/1‑93 (MQ=255)                                                                                                                ccTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATg                                                                                        <  2:266431/93‑1 (MQ=255)                                                                                                                 cTTCGGCATTACCGGGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGtct                               >  7:305045/1‑149 (MQ=255)                                                                                                                    cGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGAAGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCa                            >  2:221019/1‑149 (MQ=255)                                                                                                                            ccGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgc                    >  2:276432/1‑149 (MQ=255)                                                                                                                             cGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgcc                   <  1:276432/149‑1 (MQ=255)                                                                                                                             cGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgcc                   >  7:329895/1‑149 (MQ=255)                                                                                                                               aGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgcctg                 >  1:279757/1‑149 (MQ=255)                                                                                                                                  aaaCCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgcctgcct              <  4:268476/149‑1 (MQ=255)                                                                                                                                   aaCCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCtt             <  8:65355/149‑1 (MQ=255)                                                                                                                                   aaCCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCtt             <  2:83971/149‑1 (MQ=255)                                                                                                                                      cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAAGTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGGGTGTCCATGGAGGGGCTGCCTTGaa          <  4:143779/149‑1 (MQ=255)                                                                                                                                      cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGgcctggctgg            >  8:47308/1‑145 (MQ=255)                                                                                                                                      cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTg            <  6:75698/147‑1 (MQ=255)                                                                                                                                      cTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGaa          >  5:75698/1‑149 (MQ=255)                                                                                                                                         cttgaGCCGCGAGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGc        >  5:223181/5‑148 (MQ=255)                                                                                                                                         cttgaGCCGCGAGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCg       <  6:223181/145‑1 (MQ=255)                                                                                                                                         caacaGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGc        >  6:147612/1‑148 (MQ=255)                                                                                                                                          aacaGCCGCTCGCGGCTCAAGCCAGCAGCCATCAAGCAGACGTAATTCGAGCAGTGCGTTCAGCATGCCGATCCAAGACGATAACTATTTACTTATCACGCCTGCAAAGGCCCCATCAGCCTCTCCATGGAGCGCCTGCCTTGAAGcca      >  5:114816/1‑147 (MQ=255)                                                                                                                                             aGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCt   >  4:58228/1‑149 (MQ=255)                                                                                                                                              gCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCtt  >  5:245179/1‑149 (MQ=255)                                                                                                                                                 gCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                   >  3:11492/1‑113 (MQ=255)                                                                                                                                                 gCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                   <  4:16334/113‑1 (MQ=255)                                                                                                                                                 gCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                   <  4:11492/113‑1 (MQ=255)                                                                                                                                                 gCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGc                                   >  3:16334/1‑113 (MQ=255)                                                                                                                                                    |                                                                                                                                               CGCAGATAGCCGCCGAGGCCCAACAGGCCAAGCTTTCCGGGGTAACCCTGTACGCCACGCGCAAGAAGCTGCAGGTACGCAAATTCCCCAAACCCTGGATGCGCATGACTGCCTTCGGCATTACCGAGCAAACCTACAACAGCCGCTCGCGGCTCAAGCCAGCAGCGATCAAGCAGACGTACTTCGAGCAGTGCGTTCAGCATGCCGTTGCAAGACGATAACTCTTTACTTATCACGCCTGCAAAGGCCGCATCAGCGTCTCCATGGAGGGCCTGCCTTGAAGCGATCTT  >  NC_002947/4096503‑4096792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑