Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,128,704 G→A 6.8% A175V (GCC→GTC)  PP_3632 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,128,7040GA 6.8% 174.6 / 2.1 59A175V (GCC→GTC) PP_3632LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/31);  new base A (2/2);  total (26/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

CAGCTCGATCCGCGGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTGAGAGGTCAGCACCTGGAC  >  NC_002947/4128559‑4128849
                                                                                                                                                  |                                                                                                                                                 
cAGCTCGATCCGCGGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTcggcc                                                                                                                                                 <  7:31052/148‑1 (MQ=255)
 aGCTCGATCCGCGGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTcggcc                                                                                                                                                 >  5:351658/1‑147 (MQ=255)
 aGCTCGATCCGCGGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTcggcc                                                                                                                                                 <  6:351658/147‑1 (MQ=255)
   cTCGATCCGCGGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATAAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGca                                                                                                                                             >  2:178751/1‑149 (MQ=255)
           gcgGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGc                                                                                                                                      >  2:330635/1‑148 (MQ=255)
                  ggggTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCAGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATc                                                                                                                               <  2:145165/148‑1 (MQ=255)
                   gggTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCgg                                                                                                                             >  1:137640/1‑149 (MQ=255)
                      tACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTc                                                                                                                          <  3:62269/149‑1 (MQ=255)
                      tACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTc                                                                                                                          <  1:330635/149‑1 (MQ=255)
                           ccccGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGcgcc                                                                                                                     >  1:21789/1‑149 (MQ=255)
                                 cgcTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAgg                                                                                                               >  4:131803/1‑149 (MQ=255)
                                  gcTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAggg                                                                                                              <  5:256114/149‑1 (MQ=255)
                                   cTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAgggg                                                                                                             <  6:102370/149‑1 (MQ=255)
                                   cTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAgggg                                                                                                             <  4:23631/149‑1 (MQ=255)
                                      cAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGt                                                                                                           <  2:137640/148‑1 (MQ=255)
                                           cACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGCCCGt                                                                                                     >  4:57399/1‑149 (MQ=255)
                                             cTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGt                                                                                                     >  3:251850/1‑147 (MQ=255)
                                             cTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGt                                                                                                     >  2:180595/1‑147 (MQ=255)
                                              tCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGACCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGt                                                                                                     >  5:258583/1‑146 (MQ=255)
                                              tCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGACCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGt                                                                                                     <  8:227761/146‑1 (MQ=255)
                                              tCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGACCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGt                                                                                                     >  7:227761/1‑146 (MQ=255)
                                              tCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGACCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGt                                                                                                     <  6:258583/146‑1 (MQ=255)
                                                        cccGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCg                                                                                        >  1:103716/1‑149 (MQ=255)
                                                           gcgACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGacc                                                                                     >  5:73027/1‑149 (MQ=255)
                                                             gACAGCTGGGTGGTGCTGTCGGTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGaccac                                                                                   <  7:124613/149‑1 (MQ=255)
                                                               cAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGTGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCt                                                                                 <  8:254772/149‑1 (MQ=255)
                                                                  cTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGCGGCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCtgt                                                                               >  1:64809/1‑149 (MQ=255)
                                                                  cTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCtgtg                                                                              <  1:127650/149‑1 (MQ=255)
                                                                   tGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTgg                                                                             >  8:210555/1‑149 (MQ=255)
                                                                     ggtggtGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGc                                                                            <  1:180595/148‑1 (MQ=255)
                                                                       tggtgCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAg                                                                         <  6:73027/149‑1 (MQ=255)
                                                                       tggtgCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAg                                                                         <  2:21789/149‑1 (MQ=255)
                                                                         gtgCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGc                                                                       <  7:38914/149‑1 (MQ=255)
                                                                               tCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACaa                                                                  <  1:269933/148‑1 (MQ=255)
                                                                                 gTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCCTTGCcacaggt                                                                <  3:131803/148‑4 (MQ=255)
                                                                                  ttGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGcc                                                              <  2:103716/149‑1 (MQ=255)
                                                                                    gagaATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGa                                                            >  7:253272/1‑149 (MQ=255)
                                                                                       aaTCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGc                                                               >  8:249841/1‑143 (MQ=255)
                                                                                       aaTCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGc                                                               <  7:249841/143‑1 (MQ=255)
                                                                                           aaCGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAg                                                     >  5:357100/1‑149 (MQ=255)
                                                                                              gCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGc                                                               >  5:255796/1‑136 (MQ=255)
                                                                                              gCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGc                                                               <  6:255796/136‑1 (MQ=255)
                                                                                                        gccagccaGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACg                                         >  1:175796/1‑149 (MQ=255)
                                                                                                         ccagccagGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACg                                         >  1:237274/1‑148 (MQ=255)
                                                                                                         ccagccagGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACg                                         >  5:156802/1‑148 (MQ=255)
                                                                                                         ccagccagGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACg                                         >  7:34120/1‑148 (MQ=255)
                                                                                                          cagccagGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGc                                        >  3:294889/1‑148 (MQ=255)
                                                                                                             ccagGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAgc                                    <  2:237274/149‑1 (MQ=255)
                                                                                                              cagGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGGCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAgcg                                   <  1:178751/149‑1 (MQ=255)
                                                                                                                 gCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCAc                                <  4:251850/149‑1 (MQ=255)
                                                                                                                   cGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGa                              <  7:210555/149‑1 (MQ=255)
                                                                                                                   cGGAGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAgcgc                                  <  4:163950/145‑1 (MQ=255)
                                                                                                                   cGGAGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAgcgc                                  >  3:163950/1‑145 (MQ=255)
                                                                                                                     gggTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGc                            <  8:253272/149‑1 (MQ=255)
                                                                                                                       gTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGa                          <  2:305720/149‑1 (MQ=255)
                                                                                                                          aTCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGc                       <  2:64809/149‑1 (MQ=255)
                                                                                                                             cTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCt                     >  8:220154/1‑148 (MQ=255)
                                                                                                                                gCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTgaga                 <  8:260592/149‑1 (MQ=255)
                                                                                                                                gCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGGCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTgaga                 <  8:246214/149‑1 (MQ=255)
                                                                                                                                   ggTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTGAGAGGt              >  8:75555/1‑149 (MQ=255)
                                                                                                                                               tCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCTATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTGAGAGGTCAGCACCTGGAc  <  1:281201/149‑1 (MQ=255)
                                                                                                                                               tCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTGAGAGGTCAGCACCTGGAc  <  2:339423/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CAGCTCGATCCGCGGTTCGGGGTACAACCCCGCCGCTGCAAACCACTCGCCGGTAACCCGCGACAGCTGGGTGGTGCTGTCGTTGAGAATCAACGCCCGTTGC‑GCCAGCCAGGCCGGGGTGATCCTGGCCGGTGGCTGCCAGTCGGCCGGCACATACGCCAGGATCGGGTCGCGCCGCCAGGGGGTGACCGTCAGCCCTTTGCCGACCACCTGTGGCAAGGCCACAAGGCCGATATCCAGTGTGCCCTCACGCAAGCGCACGAGCGACGCCTGAGAGGTCAGCACCTGGAC  >  NC_002947/4128559‑4128849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑