Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,228,393 G→A 7.2% intergenic (‑137/+399) PP_5624 ← / ← PP_3707 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,228,3930GA 7.2% 205.2 / 4.1 73intergenic (‑137/+399)PP_5624/PP_3707hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/31);  new base A (2/3);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.33e-01

AACCACCCACATGTTGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTCTTGCCTTAGC  >  NC_002947/4228245‑4228541
                                                                                                                                                    |                                                                                                                                                    
aaCCACCCACATGTTGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTcgcg                                                                                                                                                      >  4:310896/1‑149 (MQ=255)
      ccacATGTTGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGc                                                                                                                                                >  3:198399/1‑149 (MQ=255)
        acaTGTTGAGCTCAGATGAATAGGAGCCAGGTATAATATCCTCCACTGTCCTTGGAGTGGCGAACATAGAAAGGTAATGTGATTTGCACAACTAGTTTTCTATTGCACCCAAGCTGGCGTCACCTATGCCGGCACGTCTCGTTATgat                                                                                                                                               >  3:224921/1‑146 (MQ=255)
         caTGTGGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCATAACGCGAc                                                                                                                                             <  8:293766/149‑1 (MQ=255)
          aTGTTGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGAc                                                                                                                                             >  8:291892/1‑148 (MQ=255)
            gTTGAGCTCAGTTGATTAGGAGGCAGGGATACTATCCTCCCCTTTCCTTGGTGTGGCGAACGTATAAAGGGACTGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGGGTCTCCTATGCCGGCACGTCGCTTTACGCGAcgt                                                                                                                                           <  2:262116/148‑3 (MQ=255)
             ttGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACaaa                                                                                                                                          <  4:301114/148‑1 (MQ=255)
             ttGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCATAACGCGAcgtgc                                                                                                                                         <  6:256462/149‑5 (MQ=255)
                      gATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTcgacga                                                                                                                                <  4:150565/149‑1 (MQ=255)
                         gAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCAGGCACGTCGCGATATGCGACAAAGCCTCGACGATAc                                                                                                                             >  2:298884/1‑149 (MQ=255)
                          aaTAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCACCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATAc                                                                                                                             >  3:334447/1‑148 (MQ=255)
                            tAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCAt                                                                                                                          >  8:144245/1‑149 (MQ=255)
                                      ggATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGTTTTGCCCAACGAGTATTATAGTGCTCCCAAGCTGGCGTATCATATCCCGGCACGTCGCGTTATGCGACAAAGCCTCGACAAAACCATTAGGCCATCa                                                                                                                >  4:272402/1‑149 (MQ=255)
                                       gATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCAc                                                                                                               >  4:19397/1‑149 (MQ=255)
                                        aTACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCAcc                                                                                                              <  7:144245/149‑1 (MQ=255)
                                         tACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACTAGTTTTATAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCt                                                                                                             <  4:198399/149‑1 (MQ=255)
                                         tACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCt                                                                                                             <  7:291892/149‑1 (MQ=255)
                                            tATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTgg                                                                                                          >  8:45128/1‑149 (MQ=255)
                                                 tccCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAAt                                                                                                     >  3:147331/1‑149 (MQ=255)
                                                    ccTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGa                                                                                                  >  3:28298/1‑149 (MQ=255)
                                                    ccTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGa                                                                                                  <  7:45128/149‑1 (MQ=255)
                                                          cTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATgg                                                                                            >  1:234356/1‑149 (MQ=255)
                                                          cTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATg                                                                                             <  3:261544/148‑1 (MQ=255)
                                                             ggAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATggg                                                                                           <  2:234356/147‑1 (MQ=255)
                                                              gAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATc                                                                                        >  7:54187/1‑149 (MQ=255)
                                                                     gAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATtct                                                                                 >  7:130571/1‑149 (MQ=255)
                                                                     gAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATtct                                                                                 >  1:179623/1‑149 (MQ=255)
                                                                      aaCCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATtctc                                                                                <  3:310896/149‑1 (MQ=255)
                                                                       aCCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTcc                                                                               >  4:25472/1‑149 (MQ=255)
                                                                            gAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGc                                                                                                                                        <  4:30248/87‑1 (MQ=255)
                                                                            gAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGc                                                                                                                                        >  3:30248/1‑87 (MQ=255)
                                                                              aaGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTg                                                                        <  8:54187/149‑1 (MQ=255)
                                                                                ggTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGa                                                                      <  1:156885/149‑1 (MQ=255)
                                                                                ggTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGa                                                                      <  3:19397/149‑1 (MQ=255)
                                                                                     ggTGATTTGCCCTACGAGTTTTCTAGTGCTCCCAATCTGGCGTCGCCTATGCCGGCACGTCGCGTTAAGCGACAAAGCCTCTACGATACGATTAGCCCATCACCTTCGTATATGGAGTTTGGGATCCGATTCTCCTGCCTTGGAGTGTc                                                                 <  4:334447/149‑1 (MQ=255)
                                                                                     ggTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAg                                                                                                                       <  6:290955/95‑1 (MQ=255)
                                                                                     ggTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAg                                                                                                                       >  5:290955/1‑95 (MQ=255)
                                                                                     ggTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTAATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTc                                                                 <  6:309251/149‑1 (MQ=255)
                                                                                      gTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCt                                                                <  3:25472/149‑1 (MQ=255)
                                                                                             gCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAg                                                          <  8:130571/148‑1 (MQ=255)
                                                                                              cccAACTAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGt                                                         <  4:115688/148‑1 (MQ=255)
                                                                                                cAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGa                                                       >  7:110217/1‑148 (MQ=255)
                                                                                                 aaCGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATa                                                     >  4:253342/1‑149 (MQ=255)
                                                                                                  aCGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATAc                                                    <  3:253342/149‑1 (MQ=255)
                                                                                                         ttCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATa                                              <  8:110217/148‑1 (MQ=255)
                                                                                                          tCTAGTGCTCCCAATCTGTCGTCTCCTATGCCGGCACGTCGCGTTATCCGACAAATCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTTGAGTGTCTGATCAGTTATACCAGATAg                                             <  4:224921/148‑1 (MQ=255)
                                                                                                            tAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATa                                              <  1:10591/145‑1 (MQ=255)
                                                                                                            tAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATa                                              >  2:10591/1‑145 (MQ=255)
                                                                                                            tAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGc                                           >  3:292092/1‑148 (MQ=255)
                                                                                                            tAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATAACAGATAGGc                                           >  1:199392/1‑148 (MQ=255)
                                                                                                              gTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACTTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGc                                           <  4:147331/146‑1 (MQ=255)
                                                                                                              gTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGcc                                          >  5:146648/1‑147 (MQ=255)
                                                                                                              gTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGcc                                          <  6:146648/147‑1 (MQ=255)
                                                                                                              gTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCAc                                        >  6:24707/1‑149 (MQ=255)
                                                                                                                 cTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATa                                              <  1:259067/140‑1 (MQ=255)
                                                                                                                 cTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATa                                              >  2:259067/1‑140 (MQ=255)
                                                                                                                   cccAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGa                                                              >  7:33083/1‑122 (MQ=255)
                                                                                                                   cccAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGa                                                              <  8:33083/122‑1 (MQ=255)
                                                                                                                   cccAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTAGGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGa                                   <  4:28298/149‑1 (MQ=255)
                                                                                                                      aaGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTc                                                                 <  3:96900/116‑1 (MQ=255)
                                                                                                                      aaGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTc                                                                 >  4:96900/1‑116 (MQ=255)
                                                                                                                       aGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACAtt                               >  8:102338/1‑149 (MQ=255)
                                                                                                                                 tcCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCgct                     >  4:319290/1‑149 (MQ=255)
                                                                                                                                 tcCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCgct                     >  6:75016/1‑149 (MQ=255)
                                                                                                                                      tGCCGGCACTTCGCTTTATTCTACAATGCCTCTACTTTACCATTTAGTCATCACCTTGGTAAATGGAGTATGGGATCTGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCgctcgg                  <  1:298884/147‑1 (MQ=255)
                                                                                                                                        ccGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCgctcggctcg              >  5:301663/1‑149 (MQ=255)
                                                                                                                                        ccGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCgctcggctcg              >  7:221042/1‑149 (MQ=255)
                                                                                                                                         cGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGt             >  3:293127/1‑149 (MQ=255)
                                                                                                                                         cGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGt             <  7:102338/149‑1 (MQ=255)
                                                                                                                                          ggCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTc            >  8:329299/1‑149 (MQ=255)
                                                                                                                                            tttgTCGCATAACGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAgt                                                                    >  7:208143/4‑91 (MQ=255)
                                                                                                                                            tttgTCGCATAACGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAgt                                                                    <  8:208143/88‑1 (MQ=255)
                                                                                                                                            tttgTCGCATAACGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTCtt          >  4:269698/4‑149 (MQ=255)
                                                                                                                                              cGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTCTTGc        <  6:301663/149‑1 (MQ=255)
                                                                                                                                                  gcgTTATGCGACAAAGACTCGACGATACCATTAGGCAATCACCTTGGTAAATGGATTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTCTTGCCTTa    <  6:271404/149‑1 (MQ=255)
                                                                                                                                                  gcgTTATGCGACAAACCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGAATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGAAATTTCAGTGAGCTCGGCTCGTCTTGCCt      >  5:271404/1‑147 (MQ=255)
                                                                                                                                                    gTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTCTTGCCTTAGc  <  2:179623/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACCACCCACATGTTGAGCTCAGATGAATAGGAGCCAGGGATACTATCCTCCCCTGTCCTTGGAGTGGCGAACCTAGAAAGGTACGGTGATTTGCCCAACGAGTTTTCTAGTGCTCCCAAGCTGGCGTCTCCTATGCCGGCACGTCGCGTTATGCGACAAAGCCTCGACGATACCATTAGGCCATCACCTTGGTAAATGGAGTATGGGATCCGATTCTCCTGCCTTGGAGTGTCTGATCAGTGATACCAGATAGGCCACTACGACATTTCCGTGCGCTCGGCTCGTCTTGCCTTAGC  >  NC_002947/4228245‑4228541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑