Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 322,133 G→A 12.7% intergenic (+420/+38) aguA → / ← PP_0267 agmatine deiminase/ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947322,1330GA12.7% 134.9 / 9.5 55intergenic (+420/+38)aguA/PP_0267agmatine deiminase/ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base G (25/23);  new base A (3/4);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.88e-01

AAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCT  >  NC_002947/321995‑322275
                                                                                                                                          |                                                                                                                                               
aaGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                       >  1:261308/1‑149 (MQ=255)
aaGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                       <  2:261308/149‑1 (MQ=255)
aaGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTCTACCTGCCTCTTCGCGGGCATGCTcgc                                                                                                                                       >  2:281275/1‑149 (MQ=255)
 aGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCcgcg                                                                                                                                      <  3:209536/149‑1 (MQ=255)
     tCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGaa                                                                                                                                    >  5:354385/1‑147 (MQ=255)
       aGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCCGCGAAGAgg                                                                                                                                <  4:301120/149‑1 (MQ=255)
               gacgacCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCATTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTa                                                                                                                          >  5:53269/1‑147 (MQ=255)
               gacgacCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCATTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTa                                                                                                                          >  5:48023/1‑147 (MQ=255)
                acgacCGCCCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGTCGGTGGGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCACGCTCGCGAAGAGGCAGGTACAg                                                                                                                       <  7:224197/149‑1 (MQ=255)
                    ccgaccCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCAGCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCaa                                                                                                                   >  7:193041/1‑149 (MQ=255)
                      gaccCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACt                                                                                                                 >  8:153803/1‑149 (MQ=255)
                        cccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACtt                                                                                                                >  2:190748/1‑148 (MQ=255)
                         ccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGAGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCcgcg                                                                                                                                      <  8:329232/125‑1 (MQ=255)
                         ccACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGAGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCcgcg                                                                                                                                      >  7:329232/1‑125 (MQ=255)
                           acgacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTaaaa                                                                                                            <  3:129689/149‑1 (MQ=255)
                             gacgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGAACGCGAAGAGGAAGGTACAGGCAACTTAAAAt                                                                                                           >  3:266244/1‑148 (MQ=255)
                              acgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCa                                                                                                         <  6:354385/149‑1 (MQ=255)
                              acgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCa                                                                                                         <  7:239656/149‑1 (MQ=255)
                              acgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCcgcg                                                                                                                                      <  8:129942/120‑1 (MQ=255)
                              acgTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGAGCATGCCcgcg                                                                                                                                      >  7:129942/1‑120 (MQ=255)
                              acgTCTGCTTGCCGGGCGATACCCCCAACGGTTGATCAGCGAAGCAGAGCAGCAGGCGCTGATGGCGGTGTGGCACTGATCGATAGCACCTGAACCAGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCa                                                                                                         <  7:234851/149‑1 (MQ=255)
                                gTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTTATCCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                       <  1:281275/149‑1 (MQ=255)
                                gTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                       <  3:21932/149‑1 (MQ=255)
                                gTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGa                                                                                                       <  7:153803/149‑1 (MQ=255)
                                  cTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGAAGCAGGCGCTGATGGAGCTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCTCGGGCATGCTCGCGAAGAGGCAGGTACATGCAACTTAAAATCAGaa                                                                                                      >  3:48057/1‑148 (MQ=39)
                                     cTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACt                                                                                                   <  2:302255/148‑1 (MQ=255)
                                                     cccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcacca                                                                                  >  2:75295/1‑149 (MQ=255)
                                                      ccAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTcacca                                                                                  >  6:72216/1‑148 (MQ=255)
                                                                aTCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCATTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgc                                                                       <  6:53269/149‑1 (MQ=255)
                                                                aTCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCATTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgc                                                                       <  6:48023/149‑1 (MQ=255)
                                                                aTCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTGCAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTAcgc                                                                       >  3:263202/1‑149 (MQ=255)
                                                                   aGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTAGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGa                                                                    >  6:120096/1‑149 (MQ=255)
                                                                           agagATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCTGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                               <  6:266811/130‑1 (MQ=255)
                                                                           agagATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACaa                                                                               >  5:266811/1‑130 (MQ=255)
                                                                           agagATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCAc                                                            >  5:3502/1‑149 (MQ=255)
                                                                             agaTGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCt                                                          >  6:53160/1‑149 (MQ=255)
                                                                                     gcgcTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTgc                                                 <  5:72216/149‑1 (MQ=255)
                                                                                        cTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGcgcc                                              <  1:190748/149‑1 (MQ=255)
                                                                                              gCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTg                                        >  7:93438/1‑149 (MQ=255)
                                                                                                      gCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAATGCTa                                                                          <  7:46640/108‑1 (MQ=255)
                                                                                                      gCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTa                                                                          >  8:46640/1‑108 (MQ=255)
                                                                                                       cACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAg                               >  8:243676/1‑149 (MQ=255)
                                                                                                        aCTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGc                              <  8:193041/149‑1 (MQ=255)
                                                                                                               gATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTgtagt                        <  7:243676/148‑1 (MQ=255)
                                                                                                                aTTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGtagtag                      >  5:38316/1‑149 (MQ=255)
                                                                                                                aTTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGtagtag                      >  5:275263/1‑149 (MQ=255)
                                                                                                                    cACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCCCAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCt                  >  3:289032/1‑149 (MQ=255)
                                                                                                                          tACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAg            <  6:38316/149‑1 (MQ=255)
                                                                                                                             cTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTc              >  2:327293/1‑144 (MQ=255)
                                                                                                                             cTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTc              <  1:327293/144‑1 (MQ=255)
                                                                                                                             cTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCaa         >  8:18259/1‑149 (MQ=255)
                                                                                                                                ccTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGATCCTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt        <  1:75295/148‑1 (MQ=255)
                                                                                                                                ccTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAAt        <  8:93438/147‑1 (MQ=255)
                                                                                                                                    ttCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTg    <  5:120096/147‑1 (MQ=255)
                                                                                                                                    ttCGCGAGCATGCCCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCt  >  1:167471/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                               
AAGCATCAGGGTCAGGACGACCGACCCACGACGTCTGCTTGCCGGGCGATACCCCCAAGGGTTGATCAGCGAAGCAGAGATGCAGGCGCTGATGGCGGTGTGGCACTGATCGATTGCACCTGTACCTGCCTCTTCGCGGGCATGCTCGCGAAGAGGCAGGTACAGGCAACTTAAAATCAGAAACTGTAGCTACCCGTCACCACAAGGCTACGCGGATCGCCCACCTGGAT‑CTGCGCCGCACTGGTCGCCGAGCTGTAGTAGGTCTTGTCAGCAATGTTGCT  >  NC_002947/321995‑322275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑