Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,287,468 C→G 7.0% G90R (GGC→CGC)  PP_3758 ← two component system response regulator/sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,287,4680CG 7.0% 213.5 / 4.1 70G90R (GGC→CGC) PP_3758two component system response regulator/sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base C (32/32);  new base G (2/3);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGAC  >  NC_002947/4287326‑4287607
                                                                                                                                              |                                                                                                                                           
tGCCGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTAGCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCcgccgc                                                                                                                                       >  2:125692/1‑149 (MQ=255)
  ccGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCgcgc                                                                                                                                     >  5:189253/1‑149 (MQ=255)
  ccGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCgcgc                                                                                                                                     >  3:346015/1‑149 (MQ=255)
    ggCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCGGGCcgcgaga                                                                                                                                   <  7:145644/149‑4 (MQ=255)
        aaGCACCTTGCGCTGGCGGAACAGGTCGACGAACCCCGAGAACTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCCTCTCCCGTCCCGCcacgaggccga                                                                                                                               >  6:251561/1‑142 (MQ=255)
        aaGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACg                                                                                                                                >  7:97158/1‑148 (MQ=255)
        aaGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACg                                                                                                                                >  5:96086/1‑148 (MQ=255)
         aGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGaa                                                                                                                              <  8:97158/149‑1 (MQ=255)
           cACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGa                                                                                                                            <  6:96086/149‑1 (MQ=255)
              cTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGa                                                                                                                                  >  2:303333/1‑140 (MQ=255)
              cTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGa                                                                                                                                  <  1:303333/140‑1 (MQ=255)
                 gcgcTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGACATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATc                                                                                                                       <  4:181590/148‑1 (MQ=255)
                  cgcTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCg                                                                                                                      <  2:344133/148‑1 (MQ=255)
                       gCGGAACAGGTCGACTAACACCGAGACCTTGCTTTTTACCGCCAGTGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAt                                                                                                                  <  5:251561/147‑1 (MQ=255)
                       gCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAtgt                                                                                                                >  6:246133/1‑149 (MQ=255)
                         ggAACAGGTCGACGAACACCGAGACCTTGCTTTTTAGCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGc                                                                                                              <  1:125692/149‑1 (MQ=255)
                         ggAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGAtgtgg                                                                                                              >  3:257114/1‑148 (MQ=255)
                         ggAACAGGTCGACCAACACCGAGACCTTGCTTTTTACCGCCACGTTGTCGAGCGGCTTGTGAAGAAAGTCCACGCCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTAGCGGCCCGCCGCGCTCACGAAGACGATCGGGATGTGc                                                                                                              >  7:139926/1‑149 (MQ=255)
                              aGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGACGTGCTTGGt                                                                                                         >  4:213726/1‑149 (MQ=255)
                                  cgacgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCGGGCcgcgaga                                                                                                                                   <  8:120543/119‑4 (MQ=255)
                                  cgacgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCGGGCcgcgaga                                                                                                                                   >  7:120543/1‑116 (MQ=255)
                                   gacgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTc                                                                                                        <  4:139253/145‑1 (MQ=255)
                                   gacgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTc                                                                                                        >  3:139253/1‑145 (MQ=255)
                                   gacgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGACCTTGAAGGAATAATTCATCTCGCGGCCCGACGCGCTGACGAAGACGATCGGGATGTGCTTGGTATTGt                                                                                                    >  4:270172/1‑149 (MQ=255)
                                    acgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTGTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTc                                                                                                   <  1:112579/149‑1 (MQ=255)
                                    acgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCGCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTc                                                                                                   <  1:235643/149‑1 (MQ=255)
                                    acgaACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTc                                                                                                   <  4:74950/149‑1 (MQ=255)
                                                aCCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCAt                                                                                       <  6:189253/149‑1 (MQ=255)
                                                  cTTGCTTTTTACCGCCAGGGTGTCGAGCGGATTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTATTGTAGGTACCGAGCAt                                                                                       >  6:340525/1‑147 (MQ=255)
                                                       tttttACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGt                                                                                                    <  5:260484/129‑1 (MQ=255)
                                                       tttttACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGt                                                                                                    >  6:260484/1‑129 (MQ=255)
                                                       tttttACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCg                                                                                >  2:289136/1‑149 (MQ=255)
                                                         tttACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCggg                                                                                                                    >  5:181048/1‑111 (MQ=255)
                                                         tttACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCggg                                                                                                                    <  6:181048/111‑1 (MQ=255)
                                                          ttACGGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCTTAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTTCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGc                                                                              <  8:139926/148‑1 (MQ=255)
                                                            aCCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACCGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCg                                                                                >  3:87347/1‑144 (MQ=255)
                                                            aCCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACAGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCg                                                                                <  4:87347/144‑1 (MQ=255)
                                                               gCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTc                                                                        <  6:326438/149‑1 (MQ=255)
                                                                 cAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCg                                                                       >  3:39979/1‑148 (MQ=255)
                                                                   gggTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTc                                                                                                   >  2:358841/1‑118 (MQ=255)
                                                                                cTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCg                                                       <  1:231772/149‑1 (MQ=255)
                                                                                    tgCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCAt                                                   <  1:289136/149‑1 (MQ=255)
                                                                                      cccccactCCCCGGCCCCCCTCCCGCACCCCATCACCCCATAATTCATCTCGCGGCCCGCCGCGCAGACGAAGACGATCCGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCt                                                 <  4:257114/142‑1 (MQ=255)
                                                                                      cAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCt                                                 >  2:15579/1‑149 (MQ=255)
                                                                                        gAAAGTCCACGGCCCCGCTCTCGTAGCCATTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGAAGAAGACGATCGGGATGTGATTGGTCTTGTGGGTACCGCGCAGCAGGTAGGCAAGGTGGAATCAGGTCATGCCCGTCATcgag                                               >  8:288588/1‑146 (MQ=255)
                                                                                         aaaGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGca                                              >  6:264086/1‑149 (MQ=255)
                                                                                           aGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGcaca                                            >  8:25309/1‑149 (MQ=255)
                                                                                            gTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACAt                                           >  1:186625/1‑149 (MQ=255)
                                                                                               cACGGCCCCGCTCTCGTAGCCCTTGAAGTCATAATTCATCTCGCGTCCCGCCGCGCTGACGAAGACGATCGTGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCga                                        <  3:270172/149‑1 (MQ=255)
                                                                                               cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTc                                                                        >  4:294769/1‑117 (MQ=255)
                                                                                               cACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTc                                                                        <  3:294769/117‑1 (MQ=255)
                                                                                                aCGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCgag                                       <  2:186625/149‑1 (MQ=255)
                                                                                                  ggCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGaa                                     <  5:246133/149‑1 (MQ=255)
                                                                                                        gCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGc                                 >  5:132873/1‑147 (MQ=255)
                                                                                                             cGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGc                           >  1:85644/1‑148 (MQ=255)
                                                                                                               tAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGaa                        >  5:308361/1‑149 (MQ=255)
                                                                                                               tAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGa                         >  3:184094/1‑148 (MQ=255)
                                                                                                                   ccTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGccc                                                        <  6:9086/113‑1 (MQ=255)
                                                                                                                   ccTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGccc                                                        >  5:9086/1‑113 (MQ=255)
                                                                                                                       gAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGACGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGc                 <  3:213726/148‑1 (MQ=255)
                                                                                                                        aaGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTc               <  6:132873/149‑1 (MQ=255)
                                                                                                                          ggCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCca             <  7:25309/149‑1 (MQ=255)
                                                                                                                             aTAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCcagca          <  5:264086/149‑1 (MQ=255)
                                                                                                                             aTAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCcagca          <  2:85644/149‑1 (MQ=255)
                                                                                                                               aaTTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCg                                                                 <  7:26080/92‑1 (MQ=255)
                                                                                                                               aaTTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCg                                                                 >  8:26080/1‑92 (MQ=255)
                                                                                                                                   cATCTGGCGGGAGGCCGCGCTGTCGAAGACGATGGGGTTGTGCTTGGTGTTGTCGGTACCGCGCATCAGCTGGGCCAGTTCGTAACCGTTCATGCCCGGCATCTGCACATCGTGAATGGGCAGTGCGAACTCGTGCTCCAGCAGCAAc     <  7:273621/148‑1 (MQ=255)
                                                                                                                                    tcagcgcgGCGGGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAAc     >  7:268231/5‑147 (MQ=255)
                                                                                                                                    tcagcgcgGCGGGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAAc     <  8:268231/143‑1 (MQ=255)
                                                                                                                                         tcgGCCCGCCGCGCTTACGAAGACGATCTTGATGTGCTTGTTCTTGTCGGTACCGCTCATCAGCTCGGACAGTTCGAAACAGTTCATGCCCGGCATCTGCACATCGAGAATTGCCAGTGCGAACTCGTGCTCCAGCAGCAACGAc  <  5:340525/144‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                           
TGCCGGCCAAGCACCTTGCGCTGGCGGAACAGGTCGACGAACACCGAGACCTTGCTTTTTACCGCCAGGGTGTCGAGCGGCTTGTGCAGAAAGTCCACGGCCCCGCTCTCGTAGCCCTTGAAGGCATAATTCATCTCGCGGCCCGCCGCGCTGACGAAGACGATCGGGATGTGCTTGGTCTTGTCGGTACCGCGCATCAGCTCGGCCAGTTCGAAACCGTTCATGCCCGGCATCTGCACATCGAGAATGGCCAGTGCGAACTCGTGCTCCAGCAGCAACGAC  >  NC_002947/4287326‑4287607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑