Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,289,435 T→A 8.1% E1051V (GAG→GTG)  PP_3761 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,289,4350TA 8.1% 142.6 / 3.2 49E1051V (GAG→GTG) PP_3761two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base A (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

TTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTGCAG  >  NC_002947/4289287‑4289580
                                                                                                                                                     |                                                                                                                                                 
ttGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTAGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCt                                                                                                                                                   >  6:213788/1‑149 (MQ=255)
      gCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCCGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAggg                                                                                                                                             >  2:329265/1‑149 (MQ=255)
      gCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAggg                                                                                                                                             <  7:301172/149‑1 (MQ=255)
               gCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGt                                                                                                                                      >  1:127320/1‑147 (MQ=255)
                 gggTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAgg                                                                                                                                  <  4:291834/149‑1 (MQ=255)
                   gTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGTGCGCCCTTGTGCTCCAGGGCGCTGGTCAggg                                                                                                                                 <  1:219380/149‑1 (MQ=255)
                           gAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGatat                                                                                                                        >  8:132136/1‑149 (MQ=255)
                           gAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTACAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTACAGGTCTCTGGGCAGGTCAAatatat                                                                                                                        >  4:315069/1‑149 (MQ=255)
                            aaGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATAtt                                                                                                                       <  6:280295/149‑1 (MQ=255)
                                       ctcCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTGGAGCACAAGGgcg                                                                                                                                           >  6:145640/1‑118 (MQ=255)
                                       ctcCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTGGAGCACAAGGgcg                                                                                                                                           <  5:145640/118‑1 (MQ=255)
                                            gCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTcc                                                                                                       <  5:12374/149‑1 (MQ=255)
                                                 atcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccacca                                                                                                   <  7:132136/148‑1 (MQ=255)
                                                 atcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACcag                                                                                                  >  3:21216/1‑149 (MQ=255)
                                                   caCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACcag                                                                                                  >  5:368159/1‑147 (MQ=255)
                                                           tCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTg                                                                                        <  2:127320/149‑1 (MQ=255)
                                                                 ccAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGccc                                                                                  >  6:364909/1‑149 (MQ=255)
                                                                  cAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCt                                                                                 >  1:16776/1‑149 (MQ=255)
                                                                    ggTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGcc                                                                                   <  3:332667/145‑1 (MQ=255)
                                                                    ggTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGcc                                                                                   >  4:332667/1‑145 (MQ=255)
                                                                    ggTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTc                                                                                >  4:53634/1‑148 (MQ=255)
                                                                     gTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCg                                                                               >  7:136950/1‑148 (MQ=255)
                                                                      tCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGCCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGaa                                                                             <  2:107773/149‑1 (MQ=255)
                                                                                       ctCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgc                                                            >  1:25587/1‑149 (MQ=255)
                                                                                         ccAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGAGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgcgc                                                          >  8:96864/1‑149 (MQ=255)
                                                                                          cAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgcgcg                                                         >  8:104276/1‑149 (MQ=255)
                                                                                          cAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgcgcg                                                         >  6:363175/1‑149 (MQ=255)
                                                                                          cAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTgcgcg                                                         >  3:140734/1‑149 (MQ=255)
                                                                                             gcgcTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCtt                                                                                         >  4:129579/1‑114 (MQ=255)
                                                                                             gcgcTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCtt                                                                                         <  3:129579/114‑1 (MQ=255)
                                                                                             gcgcTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCgg                                                      <  3:53634/149‑1 (MQ=255)
                                                                                                  cGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTg                                                 >  4:308147/1‑149 (MQ=255)
                                                                                                   gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt                                                  <  4:21216/147‑1 (MQ=255)
                                                                                                   gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt                                                  <  7:104276/147‑1 (MQ=255)
                                                                                                   gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt                                                  <  4:140734/147‑1 (MQ=255)
                                                                                                   gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGa                                                <  5:364909/149‑1 (MQ=255)
                                                                                                    aTGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAg                                               <  8:136950/149‑1 (MQ=255)
                                                                                                         ttCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGc                                          >  5:100099/1‑149 (MQ=255)
                                                                                                               cccGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGAc                                    <  1:19762/149‑1 (MQ=255)
                                                                                                               cccGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGGCTTGAGCATGCGTTGAc                                    >  4:75771/1‑149 (MQ=255)
                                                                                                                               tCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCt                     <  5:228787/148‑1 (MQ=255)
                                                                                                                                   cAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt                >  1:140052/1‑149 (MQ=255)
                                                                                                                                    aaTG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAtt               >  2:222075/1‑149 (MQ=255)
                                                                                                                                     aTG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc              <  5:213788/149‑1 (MQ=255)
                                                                                                                                         gcgcCCTGGAGCACAAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAg                                               <  2:321240/113‑1 (MQ=255)
                                                                                                                                         gcgcCCTGGAGCACAAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAg                                               >  1:321240/1‑113 (MQ=255)
                                                                                                                                           gcCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGCTGAc                                    <  3:45946/122‑1 (MQ=255)
                                                                                                                                           gcCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGCTGAc                                    >  4:45946/1‑122 (MQ=255)
                                                                                                                                                 gtgCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTtgtg     <  2:25587/147‑1 (MQ=255)
                                                                                                                                                  tgCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTGCAg  <  6:100099/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATG‑GCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTGCAG  >  NC_002947/4289287‑4289580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑