Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,298,892 T→A 8.0% intergenic (+47/+44) PP_3770 → / ← PP_3771 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,298,8920TA 8.0% 174.1 / 3.8 60intergenic (+47/+44)PP_3770/PP_3771hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (25/30);  new base A (3/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTTCGCAACGGCGAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGTTCGTTTCGTTCCTTGGCGAG  >  NC_002947/4298748‑4299034
                                                                                                                                                 |                                                                                                                                              
tGGTTCGCAACGGCGAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTgg                                                                                                                                            >  6:284127/1‑149 (MQ=255)
 ggTTCGCAACGGCGAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCGCTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTgg                                                                                                                                            >  2:37438/1‑148 (MQ=255)
  gTTCGCAACGGCGAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCGCTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTGCCTCCCTACGCCCTTGTCGCCCGCCAATTTgg                                                                                                                                            <  1:37438/148‑1 (MQ=255)
  gTTCGCAACGGCGAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCg                                                                                                                                          <  1:89/149‑1 (MQ=255)
              gAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTttc                                                                                                                              >  4:147280/1‑149 (MQ=255)
              gAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAAATTGGCGGGcg                                                                                                                                      >  3:59881/1‑140 (MQ=255)
              gAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAAATTGGCGGGcg                                                                                                                                      <  4:59881/141‑2 (MQ=255)
                           gCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGcc                                                                                                                 >  7:128180/1‑149 (MQ=255)
                           gCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGcc                                                                                                                 >  2:352974/1‑149 (MQ=255)
                               gATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCggg                                                                                                             <  3:69198/149‑1 (MQ=255)
                               gATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAAATTGGCGGGcga                                                                                                                                     <  4:133217/125‑3 (MQ=255)
                               gATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAAATTGGCGGGcga                                                                                                                                     >  3:133217/1‑123 (MQ=255)
                                aTGCCGACCCGCGCCTAAGCCCAGGCACTGGCCGAACAGTTGCAAACAGGCACTTTGGTTGCCTGAACCGAATATGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGC‑TTTTTTTCTTCGCTGATTGCCCGGGCt                                                                                                           >  1:24420/1‑149 (MQ=255)
                                         cgcgCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGcccccc                                                                                                    >  3:167751/1‑148 (MQ=255)
                                              cTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGtt                                                                                              >  6:81972/1‑149 (MQ=255)
                                               tCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGTCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGttt                                                                                             >  5:228997/1‑149 (MQ=255)
                                                 aGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTgg                                                                                           <  5:51000/149‑1 (MQ=255)
                                                    ccAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCg                                                                                        >  3:148448/1‑149 (MQ=255)
                                                      aGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCACt                                                                                      <  5:284127/149‑1 (MQ=255)
                                                       ggCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCt                                                                                      >  5:82163/1‑148 (MQ=255)
                                                       ggCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTa                                                                                     <  4:167751/149‑1 (MQ=255)
                                                          aCTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGTCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGTCCGCTACTg                                                                                  <  6:228997/149‑1 (MQ=255)
                                                          aCTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTg                                                                                  <  7:128503/149‑1 (MQ=255)
                                                              gCCGAACCGTTGCAACCACGCACTTTGGTTGACTGAACCGAATTTGTTGTCCGCGG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGccc                                                                               <  6:10152/148‑1 (MQ=255)
                                                              gCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGcc                                                                                >  8:27840/1‑147 (MQ=255)
                                                               ccGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAg                                                                             <  8:128180/149‑1 (MQ=255)
                                                                 gAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCt                                                                           >  6:81501/1‑149 (MQ=255)
                                                                     aGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACt                                                                       <  8:127714/149‑1 (MQ=255)
                                                                           aaCCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAg                                                                 >  1:24732/1‑149 (MQ=255)
                                                                             ccAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACt                                                                       <  6:195745/141‑1 (MQ=255)
                                                                             ccAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGACCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACt                                                                       >  5:195745/1‑141 (MQ=255)
                                                                              cAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCAc                                                              <  4:148448/149‑1 (MQ=255)
                                                                              cAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCAc                                                              >  5:225382/1‑149 (MQ=255)
                                                                               acgCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGc                                                                                          <  2:28277/118‑1 (MQ=255)
                                                                               aGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCAcc                                                             <  1:352974/149‑1 (MQ=255)
                                                                                         gTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGAccttgc                                                   <  3:263423/149‑1 (MQ=255)
                                                                                         gTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGAccttgc                                                   <  7:314332/149‑1 (MQ=255)
                                                                                         gTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGAccttgc                                                   <  3:147280/149‑1 (MQ=255)
                                                                                          ttGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGAccttgcc                                                  <  6:82163/149‑1 (MQ=255)
                                                                                            gCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGAccttgcctt                                                >  6:288695/1‑149 (MQ=255)
                                                                                             ccTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGAccttgccttg                                               <  5:81501/149‑1 (MQ=255)
                                                                                              cTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGcc                                                                                         >  3:135019/1‑106 (MQ=255)
                                                                                              cTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGcc                                                                                         <  4:135019/106‑1 (MQ=255)
                                                                                              cTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACcttgccttgc                                              <  6:225382/149‑1 (MQ=255)
                                                                                                 aaCCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCATACTGGTCAGCACCATGACCTTGCCTTGCAGc                                           >  4:20258/1‑149 (MQ=255)
                                                                                                    cGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCGGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCgg                                        <  3:287223/149‑1 (MQ=255)
                                                                                                    cGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCgg                                        >  2:66345/1‑149 (MQ=255)
                                                                                                    cGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCgg                                        <  5:288695/149‑1 (MQ=255)
                                                                                                        tttGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTa                                    <  2:24732/149‑1 (MQ=255)
                                                                                                           gttgtGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACg                                  <  5:81972/148‑1 (MQ=255)
                                                                                                               tgCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGtcat                             <  3:20258/149‑1 (MQ=255)
                                                                                                                   ttG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCa                                                                  >  2:138737/1‑108 (MQ=255)
                                                                                                                   ttG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCa                                                                  <  1:138737/108‑1 (MQ=255)
                                                                                                                      tatCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTGTTTTTTCTTCGCGGATTGCCCGGGATGCCCCCCTACGTTTGCCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAg                       >  7:231054/3‑149 (MQ=255)
                                                                                                                          ccTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGttcg                   >  3:255774/1‑149 (MQ=255)
                                                                                                                          ccTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGttcg                   >  8:183587/1‑149 (MQ=255)
                                                                                                                                 ccTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGTtcgtttcgtt            >  3:299966/1‑149 (MQ=255)
                                                                                                                                  aaaaaagcccgccAAATTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGttcgtttcgt             >  2:255820/7‑147 (MQ=255)
                                                                                                                                           cgccAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGTTCGTTTCGTTCCTTGGCGAg  <  4:299966/149‑1 (MQ=255)
                                                                                                                                           cgccAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGTTCGTTTCGTTCCTTGGCGAg  <  4:255774/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TGGTTCGCAACGGCGAAGCCGTGAGCGGCCCGATGCCGACCCGCGCCTCAGCCCAGGCACTGGCCGAACAGTTGCAACCAGGCACTTTGGTTGCCTGAACCGAATTTGTTGTGCGTTG‑CTCCCTACGCCCTTGTCGCCCGCCAATTTGGCGGGCTTTTTTTTCTTCGCGGATTGCCCGGGCTGCCCCCCTACGTTTGGCCGCTACTGCCCAGCTTACTGGTCAGCACCATGACCTTGCCTTGCAGCCGGCCTACGGTCATCACCAGTTCGTTTCGTTCCTTGGCGAG  >  NC_002947/4298748‑4299034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑