Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,344,310 C→G 7.8% intergenic (+41/‑115) PP_3817 → / → PP_3818 polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,344,3100CG 7.8% 157.0 / 4.0 51intergenic (+41/‑115)PP_3817/PP_3818polyamine ABC transporter ATP‑binding protein/phosphate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (24/23);  new base G (2/2);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

CCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACC  >  NC_002947/4344162‑4344454
                                                                                                                                                     |                                                                                                                                                
ccGATGCAGCCGGGCGACAGCGTGAAAGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCAccgcc                                                                                                                                                  >  1:10923/1‑149 (MQ=255)
 cGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATAGAGCAccgccc                                                                                                                                                 >  5:53957/1‑149 (MQ=255)
               gACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGAt                                                                                                                                   <  2:10923/149‑1 (MQ=255)
                 cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCt                                                                                                                                 <  5:264926/149‑1 (MQ=255)
                 cAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCt                                                                                                                                 >  2:270700/1‑149 (MQ=255)
                     gTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCa                                                                                                                             <  7:96899/149‑1 (MQ=37)
                        aaGGTGCGGTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCTCATTAACGCTCTTATACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAgg                                                                                                                           <  3:240340/148‑1 (MQ=255)
                              cGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCa                                                                                                                    >  5:267808/1‑149 (MQ=255)
                                    gCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTg                                                                                                              >  1:300877/1‑149 (MQ=255)
                                     cAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTgg                                                                                                             <  1:96728/149‑1 (MQ=255)
                                          gCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGc                                                                                                         <  6:267808/148‑1 (MQ=255)
                                                  ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGcccc                                                                                                >  2:44403/1‑149 (MQ=255)
                                                  ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGcccc                                                                                                >  1:249086/1‑149 (MQ=255)
                                                  ttGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGAGCTGGCAGGCGCTGCACTATTGGAGGCGTACGcccc                                                                                                >  4:126473/1‑149 (MQ=255)
                                                   tgaatatatcCCGAATGGGCCGAGCGCGACCTGACCAAGCCGGCGGGGGCGAATTAACGCTCTTGAAGGTTGCAGCGCGG‑GCCTTATCGCGCACCGCCCGCGCCCCACACTATCTGGCAGGCGCTGCACTATTGGAGGCGTACGcccc                                                                                                <  2:300877/142‑1 (MQ=255)
                                                    gCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAgg                                                                                                                           >  3:240771/1‑120 (MQ=255)
                                                    gCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAgg                                                                                                                           <  4:240771/120‑1 (MQ=255)
                                                              cGAATGGCCCGCCAGCCACCTGAGCACGGCGGCCGGGGCGCATTAACGCTCTTGCACGTTGCCCCGCCT‑GCCACATCGAGCACCGCCCGCGCGCCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatca                                                                                    <  7:135426/149‑1 (MQ=255)
                                                                aaTGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTatcatc                                                                                  <  1:44403/149‑1 (MQ=255)
                                                                    ggCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGc                                                                              <  1:270700/149‑1 (MQ=255)
                                                                      ccGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCa                                                                            <  2:249086/149‑1 (MQ=255)
                                                                                ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTaa                                                                   >  6:182468/1‑148 (MQ=255)
                                                                                ccTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTaa                                                                   <  5:182468/148‑1 (MQ=255)
                                                                                  tGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCTGGCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATc                                                                 >  1:264223/1‑149 (MQ=255)
                                                                                   gAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCCCCGCCCGCGCGGCACACGAGATGGCAGGCGCTGCACGATTTGCGGCGTAAGCCCCGGCCCCTTATCATCACGCCACTGTCGGActctt                                                               >  6:568/1‑148 (MQ=255)
                                                                                    cgCAAGGCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTg                                                               <  5:568/147‑1 (MQ=255)
                                                                                     gccaGTCGGCCGGGGCGCATTCACGCTCTTGTCCGTGGCAGCGCCT‑GCCCGAGCGAGCCCCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGcc                                                             <  6:178866/146‑1 (MQ=255)
                                                                                       aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTc                                                                       <  8:148067/137‑1 (MQ=255)
                                                                                       aaGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTc                                                                       >  7:148067/1‑137 (MQ=255)
                                                                                         gTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGc                                                          >  7:73160/1‑148 (MQ=255)
                                                                                            ggCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGTTCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTc                                                                       <  5:312924/132‑1 (MQ=255)
                                                                                                       aTTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCaa                                                       >  6:289927/1‑137 (MQ=255)
                                                                                                       aTTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCaa                                                       <  5:289927/137‑1 (MQ=255)
                                                                                                          aaCGCTCTTGTACGTTGCAGCGCCT‑GCCACATAGAGCACCGCCCCCGCGGCACACCATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATAACCCCACTGTCGTAATATGCCGGCCAACAGTATGCcacaag                                         >  6:201944/1‑146 (MQ=255)
                                                                                                              ctctTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCAcc                                    >  6:326415/1‑149 (MQ=255)
                                                                                                                 ttGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCCACTCTATGCCACAGGGCACCCgg                                 >  8:320409/1‑149 (MQ=255)
                                                                                                                               gcCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                   <  5:326415/149‑1 (MQ=255)
                                                                                                                               gcCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                   >  2:269896/1‑149 (MQ=255)
                                                                                                                                    gCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTg                    >  1:318993/1‑144 (MQ=37)
                                                                                                                                    gCCAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTg                    <  2:318993/144‑1 (MQ=37)
                                                                                                                                     ccAGATCGTGTGCCGCGCGGGCGGTGCTCGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc              >  2:173769/1‑149 (MQ=37)
                                                                                                                                     ccAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCTGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTc              >  4:118115/1‑149 (MQ=255)
                                                                                                                                     ccAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                   <  4:79974/144‑1 (MQ=255)
                                                                                                                                     ccAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGc                   >  3:79974/1‑144 (MQ=255)
                                                                                                                                     ccAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt               >  4:298961/1‑148 (MQ=255)
                                                                                                                                     ccAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGt               <  3:298961/148‑1 (MQ=255)
                                                                                                                                      cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCca                                             >  2:308667/1‑117 (MQ=255)
                                                                                                                                      cAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCca                                             <  1:308667/117‑1 (MQ=255)
                                                                                                                                            gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCTGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc       <  3:118115/149‑1 (MQ=255)
                                                                                                                                            gAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTtgctgc       >  3:31783/1‑149 (MQ=255)
                                                                                                                                             aGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATg                                                <  2:296685/107‑1 (MQ=255)
                                                                                                                                             aGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATg                                                >  1:296685/1‑107 (MQ=255)
                                                                                                                                              gCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTgctgct      >  2:98609/1‑148 (MQ=255)
                                                                                                                                                 ccgcccgcGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCAcc  <  8:73160/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CCGATGCAGCCGGGCGACAGCGTGAAGGTGCGTTGGGCAGTGGCAGACGCTTGCATTTATACCGAATGGGCCGAGAGCGACCTGAGCAAGTCGGCCGGGGCGCATTAACGCTCTTGTACGTTGCAGCGCCT‑GCCAGATCGAGCACCGCCCGCGCGGCACACGATCTGGCAGGCGCTGCACTATTGGAGGCGTACGCCCCGTCACATTATCATCACGCCACTGTCGTAATCTGCCGGCCAACTCTATGCCACAGGTCACCCGGCATGCTCCGCCTGCTTGTCTTGCTGCTCACC  >  NC_002947/4344162‑4344454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑