Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 326,068 T→G 10.9% A555A (GCA→GCC)  PP_0269 ← glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947326,0680TG10.9% 144.2 / 10.8 55A555A (GCA→GCC) PP_0269glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (26/23);  new base G (4/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

ATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGGT  >  NC_002947/325921‑326215
                                                                                                                                                   |                                                                                                                                                   
aTCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATg                                                                                                                                                    >  1:86259/1‑149 (MQ=255)
   aaGCTGCGTAGCTTGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCg                                                                                                                                                 >  4:212359/1‑149 (MQ=255)
   aaGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCg                                                                                                                                                 >  3:96997/1‑149 (MQ=255)
       tGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGc                                                                                                                                             >  5:304430/1‑149 (MQ=255)
             ggtCGTTATTGAGTACCACCACCGCCGGGACGCAGAACGGTTACAACGACTACGGCAACTATGTCTGCGTATTCGTCGATAACCCGATCAGCATCTTCTGATTCTGATCTGAGTTTTCTAACAGCCCCGGCCTATGCCGGGGCTGTTTc                                                                                                                                       <  8:157920/147‑1 (MQ=255)
             gCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCATAGGCCGGGGCTGttc                                                                                                                                        <  6:304430/148‑2 (MQ=255)
              cTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAAAGACGACGGCAACGAAGTCCGCGTATTCATCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGACGGGGCTGTTTCt                                                                                                                                      >  2:199469/1‑149 (MQ=255)
               gcGTGCTTGCGTACCAACACCGCCGTGCAGCAGAACTGTTACAACGACTACGGCAACTAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATTTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCtt                                                                                                                                     <  8:324781/148‑1 (MQ=255)
                         gTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAAc                                                                                                                           >  6:92750/1‑149 (MQ=255)
                         gTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAAc                                                                                                                           >  6:279321/1‑149 (MQ=255)
                              aacaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTa                                                                                                                      <  3:212359/149‑1 (MQ=255)
                               acaacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTAc                                                                                                                     >  5:50187/1‑149 (MQ=255)
                                caacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACt                                                                                                                    <  1:56981/149‑1 (MQ=255)
                                 aacGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTg                                                                                                                   >  2:27648/1‑149 (MQ=255)
                                      cGTGCAGCAGAATGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGttt                                                                                                                >  5:125655/1‑147 (MQ=255)
                                      cGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGa                                                                                                              <  8:88795/149‑1 (MQ=255)
                                                aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCg                                                                                                                                                 <  7:44710/104‑1 (MQ=255)
                                                aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCg                                                                                                                                                 >  8:44710/1‑104 (MQ=255)
                                                aaCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTg                                                                                                    >  7:170391/1‑149 (MQ=255)
                                                 aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGTTCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATTCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg                                                                                                   <  4:244505/149‑1 (MQ=255)
                                                 aCGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCATCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTgg                                                                                                   <  1:199469/149‑1 (MQ=255)
                                                     ttACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTACGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCt                                                                                                >  5:342548/1‑148 (MQ=255)
                                                       aCAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTAc                                                                                             >  4:312597/1‑149 (MQ=255)
                                                           cgacgacgGCAACGAAGTCATCGTATTCGTCGAGTACAAGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCACGGCCTATTCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTAAGGt                                                                                          >  3:60063/1‑148 (MQ=255)
                                                            gacgacgGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGtct                                                                                        <  6:125655/149‑1 (MQ=255)
                                                                 cgGCAACGAAGTCCGCGTATTCGTCGAGTACCCTATCAGCATCTTCTGATTCTGATCTGATGTATCGAACTGCCCCGGCCTATGCCGGGTCTGTTTCTTTTCGGCTAACTGTTACTGTTTGAGCCGGCGCTGGGCTTACGGGCTCCGGt                                                                                   <  2:216149/149‑1 (MQ=255)
                                                                        gAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGc                                                                            <  3:312597/149‑1 (MQ=255)
                                                                              cgcgTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGc                                                                      >  1:349894/1‑149 (MQ=255)
                                                                                    ttCGTCGAGTACCCGATCAGCATCTACTGATTCTGATCTGAAGAATCGAACATCCACGCGCTATGCCGGGGCGGTTTCTTTTCGGGCAACGGTTACTGTTTCAGACGGCGCTGGGCt                                                                                                >  7:132976/1‑117 (MQ=255)
                                                                                         cgAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATc                                                           <  5:334687/149‑1 (MQ=255)
                                                                                            gTACCCGATGAGCTTCTTGTGATTCTTATTTGATGTATCGAACAGCCCCTGCCTATGCCGGGGCTGTTTCTTTTCGTCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCg                                                         <  4:60063/148‑1 (MQ=255)
                                                                                                 cGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACAt                                                   >  5:332328/1‑149 (MQ=255)
                                                                                                  gATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATc                                                  >  8:10763/1‑149 (MQ=255)
                                                                                                        cATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCa                                            <  2:86259/149‑1 (MQ=255)
                                                                                                        cATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCa                                            >  3:58793/1‑149 (MQ=255)
                                                                                                              ctgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaaaa                                       <  6:50187/148‑1 (MQ=255)
                                                                                                              ctgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATaaaa                                       <  8:170391/148‑1 (MQ=255)
                                                                                                               tgattctgatCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACt                                     >  3:238754/1‑149 (MQ=255)
                                                                                                                     tgatctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCttt                               >  6:348168/1‑149 (MQ=255)
                                                                                                                       atctgatGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtc                              <  6:332328/148‑1 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                          >  6:235402/1‑147 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                          >  7:214234/1‑147 (MQ=255)
                                                                                                                            atGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatc                          <  8:214234/147‑1 (MQ=255)
                                                                                                                                 tCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCAc                   <  1:27648/149‑1 (MQ=255)
                                                                                                                                    aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatcga                        >  6:271827/1‑141 (MQ=255)
                                                                                                                                    aaCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTtcgatcga                        <  5:271827/141‑1 (MQ=255)
                                                                                                                                     aCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGt                                                                         >  7:220589/1‑91 (MQ=255)
                                                                                                                                     aCAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGt                                                                         <  8:220589/91‑1 (MQ=255)
                                                                                                                                      cAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg                                                                                            <  3:147653/71‑1 (MQ=255)
                                                                                                                                      cAGCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACg                                                                                            >  4:147653/1‑71 (MQ=255)
                                                                                                                                       aGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGt              >  5:231794/1‑148 (MQ=255)
                                                                                                                                        gCCCCGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCACTTTCGATCGAGCCACTGAGTaa            >  1:126810/1‑149 (MQ=255)
                                                                                                                                         ccccGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAAc           <  6:231794/149‑1 (MQ=255)
                                                                                                                                         ccccGGCATAGGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAAc           >  2:236248/1‑149 (MQ=255)
                                                                                                                                             ggCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCAc       <  5:348168/149‑1 (MQ=255)
                                                                                                                                                   tGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGGt  <  5:279321/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATCAAGCTGCGTAGCTCGTTCTTGCGTACCAACAACGCCGTGCAGCAGAACGGTTACAACGACGACGGCAACGAAGTCCGCGTATTCGTCGAGTACCCGATCAGCATCTTCTGATTCTGATCTGATGTATCGAACAGCCCCGGCCTATGCCGGGGCTGTTTCTTTTCGGCGAACGGTTACTGTTTGAGCCGGCGCTGGGCTTACGGTCTCCGGTGCAAAGCTGTCGCTACGCGCCATCCGCCACATCCGCGCATAAAACTCGCTTTCGATCGAGCCACTGAGTAACTCACCTGGT  >  NC_002947/325921‑326215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑