| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,357,746 | T→C | 22.2% | intergenic (+92/‑162) | PP_3831 → / → PP_3832 | hypothetical protein/CsrA‑like carbon storage regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,357,746 | 0 | T | C | 22.2% | 62.8 / 15.4 | 36 | intergenic (+92/‑162) | PP_3831/PP_3832 | hypothetical protein/CsrA‑like carbon storage regulator |
| Reads supporting (aligned to +/- strand): ref base T (16/12); new base C (4/4); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
GTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCA > NC_002947/4357604‑4357893 | gTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGt < 8:194503/149‑1 (MQ=255) gttTCTTCAGGCACTTGAGGAACAGGAGGACCGTTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCACATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCt > 7:280509/1‑149 (MQ=255) ttcttcAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgtcg < 3:267940/139‑4 (MQ=255) ttcttcAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgtcg > 4:267940/1‑136 (MQ=255) aGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTg > 8:185962/1‑148 (MQ=255) ggCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGaa < 4:297787/149‑1 (MQ=255) aggaCCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCtt > 6:32888/1‑115 (MQ=255) ccATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTTGCACCTGCAACTGAATACACGCAACACAATTtgact > 6:245182/1‑149 (MQ=255) gAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCATATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCg < 6:19726/148‑1 (MQ=255) aGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCgg < 8:280509/148‑1 (MQ=255) tCTATTCAGCCCATGATGCGAAGAAATTTCCCAATAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGTGAATTa < 2:71589/149‑1 (MQ=255) ttCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACt < 7:185962/147‑1 (MQ=255) cccATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACTCg < 4:64108/113‑1 (MQ=255) cccATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACg > 3:64108/1‑113 (MQ=255) gaagaaATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCt > 4:268708/1‑149 (MQ=255) aTTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATg < 5:245182/149‑1 (MQ=255) ccATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaag > 8:104991/1‑149 (MQ=255) cATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaag > 7:78275/1‑148 (MQ=255) cgccAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAtgg < 3:268708/149‑1 (MQ=255) ccAACCCACCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAAAAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaaggaa < 6:109477/142‑1 (MQ=255) ccAACCACCCTATTCTTGCCAGCGAGCACCTTGGCAGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCAGCCGAAAGCGTCTTTAATGAgaaggaa > 5:109477/1‑142 (MQ=255) cccccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGaaa > 5:337707/1‑117 (MQ=255) cccccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGaaa < 6:337707/117‑1 (MQ=255) cccccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCt > 1:241843/1‑149 (MQ=255) cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTcc > 6:294118/1‑149 (MQ=255) ttGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAAc < 7:154458/57‑1 (MQ=255) ttGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAAc > 8:154458/1‑57 (MQ=255) ttGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGAGTCTTTAATGAGAAGGAAGAGGGACAAGCTCccgccgccg > 1:265199/1‑149 (MQ=255) gCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCcgccgccgc < 7:104991/148‑1 (MQ=255) gCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCcgcg > 7:185174/1‑149 (MQ=255) ccAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCTCCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCgg > 1:253095/1‑149 (MQ=255) caaagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAATGCGTCTTTAATTAGAAGGAAGAGGGACAAGATCCCGCCGCCGCGGCAAATAGCCCAGCgg > 6:307588/6‑149 (MQ=255) aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGAc > 5:288007/4‑149 (MQ=255) aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGAc > 3:57963/4‑149 (MQ=255) aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGAc < 6:288007/146‑1 (MQ=255) aaaCCGTCAACGGTTTGCTTTGGCACCTGCAACTGACGACACGCAACACAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTtat < 5:307588/149‑1 (MQ=255) ttGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCa < 5:294118/149‑1 (MQ=255) | GTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCA > NC_002947/4357604‑4357893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |