Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,357,747	G→A	21.6%	intergenic (+93/‑161)	PP_3831 → / → PP_3832	hypothetical protein/CsrA‑like carbon storage regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,357,747	0	G	A	21.6%	66.8 / 13.4	37	intergenic (+93/‑161)	PP_3831/PP_3832	hypothetical protein/CsrA‑like carbon storage regulator
Reads supporting (aligned to +/- strand):  ref base G (16/13);  new base A (4/4);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

GTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCAC  >  NC_002947/4357604‑4357894                                                                                                                                                |                                                                                                                                                    gTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGt                                                                                                                                                <  8:194503/149‑1 (MQ=255)               gttTCTTCAGGCACTTGAGGAACAGGAGGACCGTTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCACATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCt                                                                                                                                  >  7:280509/1‑149 (MQ=255)                 ttcttcAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgtcg                                                                                                                                          <  3:267940/139‑4 (MQ=255)                 ttcttcAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTCAACGGTTTgtcg                                                                                                                                          >  4:267940/1‑136 (MQ=255)                       aGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTg                                                                                                                           >  8:185962/1‑148 (MQ=255)                        ggCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGaa                                                                                                                         <  4:297787/149‑1 (MQ=255)                                         aggaCCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCtt                                                                                                                                          >  6:32888/1‑115 (MQ=255)                                             ccATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTTGCACCTGCAACTGAATACACGCAACACAATTtgact                                                                                                    >  6:245182/1‑149 (MQ=255)                                                  gAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCATATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCg                                                                                                <  6:19726/148‑1 (MQ=255)                                                   aGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCgg                                                                                               <  8:280509/148‑1 (MQ=255)                                                         tCTATTCAGCCCATGATGCGAAGAAATTTCCCAATAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGTGAATTa                                                                                        <  2:71589/149‑1 (MQ=255)                                                             ttCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACt                                                                                      <  7:185962/147‑1 (MQ=255)                                                                  cccATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACTCg                                                                                                                   <  4:64108/113‑1 (MQ=255)                                                                  cccATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACg                                                                                                                   >  3:64108/1‑113 (MQ=255)                                                                            gaagaaATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCt                                                                     >  4:268708/1‑149 (MQ=255)                                                                                  aTTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATg                                                               <  5:245182/149‑1 (MQ=255)                                                                                       ccATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaag                                                          >  8:104991/1‑149 (MQ=255)                                                                                        cATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaag                                                          >  7:78275/1‑148 (MQ=255)                                                                                               cgccAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAtgg                                                  <  3:268708/149‑1 (MQ=255)                                                                                                 ccAACCCACCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAAAAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAgaaggaa                                                       <  6:109477/142‑1 (MQ=255)                                                                                                 ccAACCACCCTATTCTTGCCAGCGAGCACCTTGGCAGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCAGCCGAAAGCGTCTTTAATGAgaaggaa                                                       >  5:109477/1‑142 (MQ=255)                                                                                                      cccccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGaaa                                                                           >  5:337707/1‑117 (MQ=255)                                                                                                      cccccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGaaa                                                                           <  6:337707/117‑1 (MQ=255)                                                                                                      cccccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCt                                           >  1:241843/1‑149 (MQ=255)                                                                                                        cccTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTcc                                         >  6:294118/1‑149 (MQ=255)                                                                                                                ttGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAAc                                                                                                                             >  8:154458/1‑57 (MQ=255)                                                                                                                ttGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAAc                                                                                                                             <  7:154458/57‑1 (MQ=255)                                                                                                                ttGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGAGTCTTTAATGAGAAGGAAGAGGGACAAGCTCccgccgccg                                 >  1:265199/1‑149 (MQ=255)                                                                                                                  gCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCcgccgccgc                                <  7:104991/148‑1 (MQ=255)                                                                                                                  gCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCcgcg                               >  7:185174/1‑149 (MQ=255)                                                                                                                   ccAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCTCCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCgg                              >  1:253095/1‑149 (MQ=255)                                                                                                                                  caaagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAATGCGTCTTTAATTAGAAGGAAGAGGGACAAGATCCCGCCGCCGCGGCAAATAGCCCAGCgg               >  6:307588/6‑149 (MQ=255)                                                                                                                                    aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGAc             >  5:288007/4‑149 (MQ=255)                                                                                                                                    aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGAc             >  3:57963/4‑149 (MQ=255)                                                                                                                                    aagcAAACCGTCAACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGAc             <  6:288007/146‑1 (MQ=255)                                                                                                                                        aaaCCGTCAACGGTTTGCTTTGGCACCTGCAACTGACGACACGCAACACAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTtat         <  5:307588/149‑1 (MQ=255)                                                                                                                                              ttGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCa   <  5:294118/149‑1 (MQ=255)                                                                                                                                                gACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCAc  >  8:279141/1‑148 (MQ=255)                                                                                                                                                gACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTAAGACATCAc  >  2:154079/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                    GTGGCCTTGCTGTTGTTTCTTCAGGCACTTGAGGAACAGGAGGACCATTGAGCTTGTCTATTCAGCCCATGATGCGAAGAAATTTCCCATTAGCCGCCAACCCCCCTATTCTTGCCAGCGAGCACCTTGGCCGACAAACCGTTGACGGTTTGCTTTGGCACCTGCAACTGAAGACACGCAACAGAATTTGTCTGTCGGGGAATTACTATCCGCCGAAAGCGTCTTTAATGAGAAGGAAGAGGGACAAGCTCCCGCCGCCGCGGCAACTAGCCCAGCGGACTTATACATCAC  >  NC_002947/4357604‑4357894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑