Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,442,628 C→G 11.1% intergenic (‑98/+40) nicP‑II ← / ← nicT porin‑like protein/metabolite transport protein NicT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,442,6280CG11.1% 95.8 / 5.2 36intergenic (‑98/+40)nicP‑II/nicTporin‑like protein/metabolite transport protein NicT
Reads supporting (aligned to +/- strand):  ref base C (13/19);  new base G (2/2);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

GGCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGT  >  NC_002947/4442488‑4442775
                                                                                                                                            |                                                                                                                                                    
ggCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGc                                                                                                                                              <  7:321833/149‑1 (MQ=255)
ggCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGc                                                                                                                                              <  1:263719/149‑1 (MQ=255)
    tGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGTGGGCCCCGGCCCGcct‑gc                                                                                                                                         <  8:91281/149‑1 (MQ=255)
     gCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct‑gcc                                                                                                                                        >  8:210382/1‑149 (MQ=255)
     gCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct‑gcc                                                                                                                                        >  2:202869/1‑149 (MQ=255)
        cccgcacaaaaaCGCCGCGCACGGCAACCCTTCCTACGGCCACCCCCGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGcct‑gccctg                                                                                                                                     <  1:60390/142‑1 (MQ=255)
           gaagaaAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCg                                                                                                                                  >  2:58435/1‑149 (MQ=255)
             agaaAACCGCGGCGCAGTGAAACCTTGCCTAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGtt                                                                                                                                <  8:188809/149‑1 (MQ=255)
                   aCGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTg                                                                                                                           >  1:100606/1‑148 (MQ=255)
                             gTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGc                                                                                                                <  7:179300/149‑1 (MQ=255)
                              tGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGcc                                                                                                               >  6:32563/1‑149 (MQ=255)
                               gAAACCTTGGATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGc                                                                                                                <  4:14761/147‑1 (MQ=255)
                                        cATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGGCTGGCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGt                                                                                                      <  1:49311/149‑1 (MQ=255)
                                        cATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGtt                                                                                                     <  7:210382/149‑1 (MQ=255)
                                                  cTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTg                                                                                           <  1:58435/149‑1 (MQ=255)
                                                     cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcct‑g                                                                                                                                          <  7:323402/99‑1 (MQ=255)
                                                     cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCGGGGCCCGcct‑g                                                                                                                                          >  8:323402/1‑99 (MQ=255)
                                                     cTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGc                                                                                         <  3:130690/148‑1 (MQ=255)
                                                           ttGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCaa                                                                                   <  2:100606/148‑1 (MQ=255)
                                                             gggCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGc                                                                                >  3:224271/1‑149 (MQ=255)
                                                                cAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGAt                                                                             <  4:224271/149‑1 (MQ=255)
                                                                      cAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCt                                                                                                                    <  6:196334/104‑1 (MQ=255)
                                                                      cAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCt                                                                                                                    >  5:196334/1‑104 (MQ=255)
                                                                                            aTGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGGTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAg                                                 <  4:256522/149‑1 (MQ=255)
                                                                                              ggCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGcc                                               >  5:369174/1‑149 (MQ=255)
                                                                                                  aCCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGc                                                                                >  7:314049/1‑112 (MQ=255)
                                                                                                  aCCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGc                                                                                <  8:314049/112‑1 (MQ=255)
                                                                                                  aCCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCACGAAGCAGCTGAGGATCAGGCAGCCGGCa                                           <  1:202869/149‑1 (MQ=255)
                                                                                                       cGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCaa                                          <  1:142042/145‑1 (MQ=255)
                                                                                                          gCGGGTAGCATCGAGAGAGGTCGGCAGGCGGGCCCCCGCCCGCCT‑GCCCTGCAGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGCGGATGACCACGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCca                                   >  7:202283/1‑149 (MQ=255)
                                                                                                                   aTCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCAcagcgcagc                          >  5:131911/1‑149 (MQ=255)
                                                                                                                    tCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAg                                                     >  3:68158/1‑121 (MQ=255)
                                                                                                                    tCGAGAGAGGGCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAATCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAg                                                     <  4:68158/121‑1 (MQ=255)
                                                                                                                           agGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTg                                                             <  4:251534/106‑1 (MQ=255)
                                                                                                                           agGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTg                                                             >  3:251534/1‑106 (MQ=255)
                                                                                                                                    ggCGGGCCGGGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGt           >  3:127706/1‑147 (MQ=255)
                                                                                                                                          ccccGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCgg   <  6:131911/149‑1 (MQ=255)
                                                                                                                                           cccGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAag                                                                   >  2:255989/1‑84 (MQ=255)
                                                                                                                                           cccGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAag                                                                   <  1:255989/84‑1 (MQ=255)
                                                                                                                                           cccGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGt  <  5:32563/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GGCGTGCAGACGAAGAAAAACGCGGCGCAGTGAAACCTTGCATAGGGCTACTCCTGGATTTGGGCAACAGCAACCCGGCGCGGCGGCACTGCATGGCCACCGGCGTGCGGGTTGCATCGAGAGAGGTCGGCAGGCGGGCCCCGGCCCGCCT‑GCCCTGCCGTTGCGTGAAGCGCTGGGCCGCTAGCGGTTGACCAGCTTGGCCGGCAAGGCGATGACCAGGAAGCAGCTGAGGATCAGGCAGCCGGCAAACACCCACAGCGCAGCGTGCGAGGTGCCGGTGAGGTCGGT  >  NC_002947/4442488‑4442775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑