Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,548,652 G→T 7.1% A322S (GCC→TCC) ‡ pydB → bifunctional D‑hydantoinase/dihydropyrimidinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,548,6520GT 7.1% 175.3 / 2.9 56A322S (GCC→TCC) ‡pydBbifunctional D‑hydantoinase/dihydropyrimidinase
Reads supporting (aligned to +/- strand):  ref base G (25/27);  new base T (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

TCTACCGTGACCCGGACTGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATTCGTCGCGCTGACCTCT  >  NC_002947/4548509‑4548795
                                                                                                                                               |                                                                                                                                               
tCTACCGTCACCCGGACTGGCCCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGa                                                                                                                                             <  8:81266/148‑1 (MQ=255)
      gTGACCCCGACTGGACCACCTCCGCCGGTTATGGTATGAGCCAGCAGTTCCGCCCACGAGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGAGCCGAGCAGaaa                                                                                                                                      <  8:192238/149‑1 (MQ=255)
         aCCCGGACTGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGc                                                                                                                                    <  5:234865/148‑1 (MQ=255)
          cccGGACTGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCa                                                                                                                                  <  1:164272/149‑1 (MQ=255)
          cccGGACTGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCa                                                                                                                                  >  6:5993/1‑149 (MQ=255)
           ccGGACTGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCAt                                                                                                                                 >  1:14407/1‑149 (MQ=255)
                 tGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCcg                                                                                                                           >  7:69606/1‑149 (MQ=255)
                   gACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCcgc                                                                                                                          >  2:144668/1‑148 (MQ=255)
                      caccGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCTCGGCGCAGaagca                                                                                                                                    <  8:149818/135‑4 (MQ=255)
                      caccGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCTCGGCGCAGaagca                                                                                                                                    >  7:149818/1‑132 (MQ=255)
                       accGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGaa                                                                                                                                       >  1:314778/1‑131 (MQ=255)
                       accGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGaa                                                                                                                                       <  2:314778/131‑1 (MQ=255)
                        ccgccgccgGTTATGTGATTAGCCCGCCGTTCCGCACACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCgacgac                                                                                                                    <  3:38540/149‑1 (MQ=255)
                           ccgccgGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGcga                                                                                                                        <  4:306059/142‑1 (MQ=255)
                           ccgccgGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGcga                                                                                                                        >  3:306059/1‑142 (MQ=255)
                                   tATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCTCGGCGCAGaag                                                                                                                                      <  2:2378/120‑2 (MQ=255)
                                   tATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCTCGGCGCAGaag                                                                                                                                      >  1:2378/1‑119 (MQ=255)
                                      gtgATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATccc                                                                                                      >  7:101484/1‑149 (MQ=255)
                                         atgaGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCaa                                                                                                   >  2:257355/1‑149 (MQ=255)
                                          tgaGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAAc                                                                                                  <  3:311528/149‑1 (MQ=255)
                                          tgaGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAAc                                                                                                  >  1:327337/1‑149 (MQ=255)
                                                    gTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCAccgccg                                                                                        <  8:69606/149‑1 (MQ=255)
                                                     ttCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCgg                                                                                       <  8:101484/149‑1 (MQ=255)
                                                      tCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCgg                                                                                       >  3:55680/1‑148 (MQ=255)
                                                       ccGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGc                                                                                      <  6:291682/148‑1 (MQ=255)
                                                             aCGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCg                                                                                  >  4:238545/1‑146 (MQ=255)
                                                                 gAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCg                                                                           >  7:41848/1‑149 (MQ=255)
                                                                 gAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCg                                                                           >  6:256249/1‑149 (MQ=255)
                                                                  aaCACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGc                                                                          <  4:55680/149‑1 (MQ=255)
                                                                  aaCACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGc                                                                          <  5:5993/149‑1 (MQ=255)
                                                                   acacCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCa                                                                         >  3:190094/1‑149 (MQ=255)
                                                                       cAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATgg                                                                      >  8:124942/1‑148 (MQ=255)
                                                                                cTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACtgtg                                                            <  2:327337/149‑1 (MQ=255)
                                                                                cTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACtgtg                                                            >  6:359493/1‑149 (MQ=255)
                                                                                     gcgcGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGAt                                                        <  3:238545/148‑1 (MQ=255)
                                                                                     gcgcGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATg                                                       <  2:14407/149‑1 (MQ=255)
                                                                                     gcgcGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATg                                                       <  5:256249/149‑1 (MQ=255)
                                                                                      cgcgGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGa                                                         <  2:60214/146‑1 (MQ=255)
                                                                                      cgcgGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGa                                                         >  1:60214/1‑146 (MQ=255)
                                                                                        cgGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCg                                                    <  8:41848/149‑1 (MQ=255)
                                                                                        cgGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCg                                                    <  7:124942/149‑1 (MQ=255)
                                                                                           cTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCgggg                                                 >  6:297281/1‑149 (MQ=255)
                                                                                                aGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCaa                                             >  1:331344/1‑148 (MQ=255)
                                                                                                    gggCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCTCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCg                                        >  8:271722/1‑149 (MQ=255)
                                                                                                            tGCACACAACCGCCACCGACCACTGCTGCTTCTGCGAAGAGCAGATAGCCATCGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCTCATGGCAGTACTGTGGTATGCCCTGGTCAACAGCCGGCGTTTg                                >  1:251537/1‑149 (MQ=255)
                                                                                                              cacaCCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTc                              <  7:88422/149‑1 (MQ=255)
                                                                                                              cacaCCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTc                              <  1:144668/149‑1 (MQ=255)
                                                                                                                 accaccGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTa                                                                 <  6:106975/111‑1 (MQ=255)
                                                                                                                 accaccGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTa                                                                 >  5:106975/1‑111 (MQ=255)
                                                                                                                         caccgACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAAt                   <  5:359493/149‑1 (MQ=255)
                                                                                                                         caccgACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTCTGGGATGCCGGGGTCAACTGCGGGCGTTTGGCGATGCACGAAt                   >  2:296344/1‑149 (MQ=255)
                                                                                                                               ccACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATtcgtcg             <  1:257355/149‑1 (MQ=255)
                                                                                                                                  cttctgctTCTGCGCCGAGAAGAAAGCCATGGGCCGCGACTACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCTACGACCGCATGGCAGTACTGTGGGATGCCTGGTTCAACAGCGGGCGTTTGTCGATGCACGATTTCGTagcgg          <  2:251537/146‑2 (MQ=255)
                                                                                                                                      tgctTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATTCGTCGCGCTGAc      >  3:248203/1‑149 (MQ=255)
                                                                                                                                        ctTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATTCGTCGCGCTGACct    >  5:322950/1‑149 (MQ=255)
                                                                                                                                           cTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATTCGTCGCGCTGACctct  <  6:322950/148‑1 (MQ=255)
                                                                                                                                             gcgcCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATtcgt               <  7:32868/133‑1 (MQ=255)
                                                                                                                                             gcgcCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATtcgt               >  8:32868/1‑133 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TCTACCGTGACCCGGACTGGACCACCGCCGCCGGTTATGTGATGAGCCCGCCGTTCCGCCCACGCGAACACCAGGAGGCGCTGTGGCGCGGCTTGCAGTCGGGCAACCTGCACACCACCGCCACCGACCACTGCTGCTTCTGCGCCGAGCAGAAAGCCATGGGCCGCGACGACTTCAGCCGCATCCCCAACGGCACCGCCGGCATCGAAGACCGCATGGCAGTACTGTGGGATGCCGGGGTCAACAGCGGGCGTTTGTCGATGCACGAATTCGTCGCGCTGACCTCT  >  NC_002947/4548509‑4548795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑