Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,634,356 T→A 7.1% D252V (GAC→GTC)  uvrC ← UvrABC system protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,634,3560TA 7.1% 160.5 / 2.1 56D252V (GAC→GTC) uvrCUvrABC system protein C
Reads supporting (aligned to +/- strand):  ref base T (30/22);  new base A (2/2);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

GGACAGGAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTG  >  NC_002947/4634211‑4634504
                                                                                                                                                 |                                                                                                                                                    
ggACAGGAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACgt                                                                                                                                                   >  2:223173/1‑148 (MQ=255)
    aGGAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATc                                                                                                                                               <  8:287373/149‑1 (MQ=255)
      gAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATcgc                                                                                                                                             >  4:105089/1‑149 (MQ=255)
        aTGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGTCGACGTCATcgccg                                                                                                                                           <  1:223173/149‑1 (MQ=255)
            ggCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTgc                                                                                                                                       >  8:43649/1‑149 (MQ=255)
                            aCCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTg                                                                                                                       >  4:83564/1‑149 (MQ=255)
                              ctcctcctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGtt                                                                                                                     >  6:52288/1‑149 (MQ=255)
                                  tcctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTc                                                                                                                 >  3:12221/1‑149 (MQ=255)
                                    ctcGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCt                                                                                                               >  5:183833/1‑149 (MQ=255)
                                            ccACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCg                                                                                                                               <  1:54402/125‑1 (MQ=255)
                                            ccACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCg                                                                                                                               >  2:54402/1‑125 (MQ=255)
                                             cACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACgcggc                                                                                                      <  3:83564/149‑1 (MQ=255)
                                                            gTTCTTGCTGCCCAGTACTCGCCCGCCGCCCACGCTGATCAGGTGCACACAGGCACAACCCGGGTTGACGAAGGCGGCGATGACGTAGACATCGCCGCTGCCGCCCTCGATGTACTGTAGGTCCTGCACGCGGCGCAACAAGGCGATCt                                                                                       >  2:279207/1‑149 (MQ=255)
                                                              tCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTgg                                                                                     >  3:130360/1‑149 (MQ=255)
                                                                  gctgcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTcgc                                                                                 >  5:155599/1‑149 (MQ=255)
                                                                  gctgcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTcgc                                                                                 >  3:219525/1‑149 (MQ=255)
                                                                    tgcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTg                                                                                                                                        <  1:81067/92‑1 (MQ=255)
                                                                    tgcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTg                                                                                                                                        >  2:81067/1‑92 (MQ=255)
                                                                    tgcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTa                                                                                                                          <  2:237116/106‑1 (MQ=255)
                                                                    tgcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTa                                                                                                                          >  1:237116/1‑106 (MQ=255)
                                                                     gcCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTcgcg                                                                                >  8:292657/1‑147 (MQ=255)
                                                                      cccAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCgcgc                                                                               <  7:43649/147‑1 (MQ=255)
                                                                      cccAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAg                                                                             <  3:105089/149‑1 (MQ=255)
                                                                             cTCGCCCCCCGCGCACCCGCATCAGCTGCCCACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCACTCGATGTACTGGTGGTACTGCACGCGGCGCAGCAAGGCGATCTGGACGCGCACCgcggcg                                                                      >  6:197180/1‑149 (MQ=255)
                                                                                gcccgccGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCt                                                                   >  2:294537/1‑149 (MQ=255)
                                                                                     ccgcGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTc                                                              >  2:187852/1‑149 (MQ=255)
                                                                                       gcgcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAgg                                                                                             <  1:173324/116‑1 (MQ=255)
                                                                                       gcgcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAgg                                                                                             >  2:173324/1‑116 (MQ=255)
                                                                                       gcgcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCg                                                             >  3:317320/1‑148 (MQ=255)
                                                                                         gcACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGaaa                                                          <  4:12221/149‑1 (MQ=255)
                                                                                          cACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAAt                                                         >  7:193544/1‑149 (MQ=255)
                                                                                                    aGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCAt                                               >  4:259194/1‑149 (MQ=255)
                                                                                                         cacacaGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATggcg                                           <  4:317320/148‑1 (MQ=255)
                                                                                                           cacaGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGcc                                        <  6:183833/149‑1 (MQ=255)
                                                                                                              aGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTGGATGTACTGTTTGTCCTGCAGGCGGCGCAACATGGCGTTCTGGTCGCGGAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCttt                                     <  1:279207/149‑1 (MQ=255)
                                                                                                              aGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCTGCCttt                                     >  8:330912/1‑149 (MQ=255)
                                                                                                               ggCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCtttt                                    <  2:212680/149‑1 (MQ=255)
                                                                                                               ggCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCtttt                                    >  6:108066/1‑149 (MQ=255)
                                                                                                               ggCACCACCCGGGTTGACGAAGGCGGCGATAACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCtttt                                    >  1:212680/1‑149 (MQ=255)
                                                                                                                gCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTc                                   >  2:27915/1‑149 (MQ=255)
                                                                                                                       ccGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCg                            <  3:259194/149‑1 (MQ=255)
                                                                                                                         gggTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTGGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGATATCGAGGGCCATGGCGGCCTTTTCCATCTCGGc                          <  5:197180/149‑1 (MQ=255)
                                                                                                                         gggTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACgcggcg                                                                                                     <  6:355001/74‑1 (MQ=255)
                                                                                                                         gggTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACgcggcg                                                                                                     >  5:355001/1‑74 (MQ=255)
                                                                                                                               aCGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCaa                    >  8:64603/1‑149 (MQ=255)
                                                                                                                                     gcggcgATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCAtt              <  5:52288/149‑1 (MQ=255)
                                                                                                                                      cggcgATGTCGACGTCATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCtttc                                    <  7:116378/126‑2 (MQ=255)
                                                                                                                                      cggcgATGTCGACGTCATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCtttc                                    >  8:116378/1‑125 (MQ=255)
                                                                                                                                         cgATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCAtc                               >  8:320735/1‑128 (MQ=255)
                                                                                                                                          gATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGtt                       >  5:32358/1‑135 (MQ=255)
                                                                                                                                          gATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGtt                       <  6:32358/135‑1 (MQ=255)
                                                                                                                                             gACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGtt      >  6:216571/1‑149 (MQ=255)
                                                                                                                                              aCGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGtt      <  7:292657/148‑1 (MQ=255)
                                                                                                                                              aCGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGtt      <  8:193544/148‑1 (MQ=255)
                                                                                                                                              aCGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTg     <  5:108066/149‑1 (MQ=255)
                                                                                                                                                gTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCt   <  6:107810/149‑1 (MQ=255)
                                                                                                                                                 tCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTg  <  4:130360/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGACAGGAATGCGGCCATGACCTCGGCCACCTCCTCCTCGATGCCCACCTGCGGGAAGAAGTTCTTGCTGCCCAGTACTCGCCCGCCGCGCACGCTGATCAGGTGCACACAGGCACCACCCGGGTTGACGAAGGCGGCGATGACGTCGACATCGCCGCTGCCGCCCTCGATGTACTGTTGGTCCTGCACGCGGCGCAACAAGGCGATCTGGTCGCGCAGCTCGGCGGCTTTTTCGAAATCGAGGGCCATGGCGGCCTTTTCCATCTCGGCGTTCAACTCATTGCCCAGTTGCTG  >  NC_002947/4634211‑4634504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑