Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,664,686 C→A 7.7% T280N (ACC→AAC)  nuoH → NADH‑quinone oxidoreductase subunit H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,664,6860CA 7.7% 159.7 / 4.1 52T280N (ACC→AAC) nuoHNADH‑quinone oxidoreductase subunit H
Reads supporting (aligned to +/- strand):  ref base C (24/24);  new base A (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CGGCATGAAGTGGGGCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACAC  >  NC_002947/4664540‑4664833
                                                                                                                                                  |                                                                                                                                                   
cGGCATGAAGTGGGGCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGAAcg                                                                                                                                                   >  7:125702/1‑149 (MQ=255)
cGGCATGAAGTGGGGCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGAAcg                                                                                                                                                   >  7:130616/1‑149 (MQ=255)
      gAAGTGGGGCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttct                                                                                                                                             >  4:39924/1‑149 (MQ=255)
         gTGGGGCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTtca                                                                                                                                          <  3:39924/149‑1 (MQ=255)
           ggggCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGAACGCGGTCTtcagg                                                                                                                                        <  8:125702/149‑3 (MQ=255)
            gggCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTtcatca                                                                                                                                       >  3:61304/1‑149 (MQ=255)
            gggCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGAACGCGGTCTtcagg                                                                                                                                        <  8:130616/148‑3 (MQ=255)
                 tGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTg                                                                                                                                  >  4:259000/1‑149 (MQ=255)
                               gAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTgcg                                                                                                                    >  2:303274/1‑149 (MQ=255)
                                    cATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCATCTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGcc                                                                                                                <  3:259000/148‑1 (MQ=255)
                                     aTCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttcttc                                                                                                                                           <  5:44679/120‑1 (MQ=255)
                                     aTCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttcttc                                                                                                                                           >  6:44679/1‑120 (MQ=255)
                                     aTCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCt                                                                                                                                   >  1:61590/1‑128 (MQ=255)
                                     aTCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCt                                                                                                                                   <  2:61590/128‑1 (MQ=255)
                                     aTCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTc                                                                                                              >  6:359376/1‑149 (MQ=255)
                                       cGGCCTCATCCTCATCTCGGCGCTGCCGGTAACCCTCGCCTTCCGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGa                                                                                                            <  6:111554/149‑1 (MQ=255)
                                        ggCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGAt                                                                                                           <  1:315622/149‑1 (MQ=255)
                                          catcatCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTgcgc                                                                                                                   >  2:198773/1‑139 (MQ=255)
                                          catcatCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTgcgc                                                                                                                   <  1:198773/139‑1 (MQ=255)
                                          catcatCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATcc                                                                                                         >  3:326597/1‑149 (MQ=255)
                                                  tCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCCGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCa                                                                                                 >  8:150712/1‑149 (MQ=255)
                                                         ggCGCCGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTAt                                                                                           >  7:106710/1‑148 (MQ=255)
                                                              cgctgGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAg                                                                                     <  8:106710/148‑1 (MQ=255)
                                                                ctgGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAg                                                                                     <  5:359376/147‑1 (MQ=255)
                                                                ctgGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAg                                                                                     <  1:303274/147‑1 (MQ=255)
                                                                 tgGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttc                                                                                                                                              >  2:48505/1‑89 (MQ=255)
                                                                 tgGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttc                                                                                                                                              <  1:48505/89‑1 (MQ=255)
                                                                   gTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttctt                                                                                                                                            <  7:254153/89‑1 (MQ=255)
                                                                   gTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGttctt                                                                                                                                            >  8:254153/1‑89 (MQ=255)
                                                                   gTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGAt                                                                                <  3:176018/149‑1 (MQ=255)
                                                                        ccTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAg                                                                                     <  1:64472/139‑1 (MQ=255)
                                                                        ccTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAg                                                                                     >  2:64472/1‑139 (MQ=255)
                                                                        ccTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACt                                                                           >  3:323951/1‑149 (MQ=255)
                                                                        ccTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTATCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACt                                                                           >  2:337261/1‑149 (MQ=255)
                                                                              cttcGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAgg                                                                                    <  8:122126/134‑1 (MQ=255)
                                                                              cttcGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAgg                                                                                    >  7:122126/1‑134 (MQ=255)
                                                                               ttcGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTtcatc                                                                                                                                        <  4:23360/81‑1 (MQ=255)
                                                                               ttcGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTtcatc                                                                                                                                        >  3:23360/1‑81 (MQ=255)
                                                                                tcGGCGGCTGGATCGGCCGGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTGCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTg                                                                    <  4:61304/148‑1 (MQ=255)
                                                                                 cggcggCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCt                                                                     <  4:323951/146‑1 (MQ=255)
                                                                                    cggcTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGCCCAGGGGATGGACTGCAGCTGGAAGt                                                               >  2:77481/1‑149 (MQ=255)
                                                                                                         cATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGa                                          >  6:59693/1‑149 (MQ=255)
                                                                                                                  gCAACTATCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGc                                 <  1:337261/149‑1 (MQ=255)
                                                                                                                    aaCTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTg                               <  4:326597/149‑1 (MQ=255)
                                                                                                                    aaCTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTg                               >  5:145956/1‑149 (MQ=255)
                                                                                                                           gTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCg                        >  5:154106/1‑149 (MQ=255)
                                                                                                                                tgtgGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCaa                                      <  3:270619/130‑1 (MQ=255)
                                                                                                                                tgtgGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCaa                                      >  4:270619/1‑130 (MQ=255)
                                                                                                                                          ccTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGctct         <  6:154106/149‑1 (MQ=255)
                                                                                                                                            tGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTg             >  3:66818/1‑143 (MQ=255)
                                                                                                                                            tGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTg             <  4:66818/143‑1 (MQ=255)
                                                                                                                                                 aCCGCGTTCTTCATCATGCCGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGATCGCTGCGATCGTGCTCTACAacac  <  7:150712/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGGCATGAAGTGGGGCATGTTCTTCGTCGGTGAGTACATCGGCATCATCCTCATCTCGGCGCTGCTGGTAACCCTGTTCTTCGGCGGCTGGCACGGCCCGTTCGGCATCCTGCCGCAACTGTCGTTCCTGTGGTTCGCCCTGAAGACCGCGTTCTTCATCATGCTGTTCATCCTGCTGCGCGCCTCGATCCCGCGCCCACGCTATGACCAGGTGATGGACTTCAGCTGGAAGTTCTGCCTGCCGCTGACCCTGATCAATTTGCTGGTGACCGCTGCGATCGTGCTCTACAACAC  >  NC_002947/4664540‑4664833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑