Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,669,731 G→C 7.4% T21T (ACG→ACC)  nuoN → NADH‑quinone oxidoreductase subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,669,7310GC 7.4% 161.3 / 3.3 54T21T (ACG→ACC) nuoNNADH‑quinone oxidoreductase subunit N
Reads supporting (aligned to +/- strand):  ref base G (27/23);  new base C (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

CTCTGCCGCCACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCT  >  NC_002947/4669589‑4669865
                                                                                                                                              |                                                                                                                                      
ctctGCCGCCACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACggtggtg                                                                                                                                  >  6:212460/1‑149 (MQ=255)
ctctGCCGCCACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACggtggtg                                                                                                                                  <  3:345074/149‑1 (MQ=255)
 tctGCCGCCACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACggtggtgg                                                                                                                                 >  3:26955/1‑149 (MQ=255)
   tGCCGCCACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACggtggtgg                                                                                                                                 <  4:23810/147‑1 (MQ=255)
     ccgccACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGAt                                                                                                                             >  7:256501/1‑149 (MQ=255)
         caccaTGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTg                                                                                                                         <  5:28045/149‑1 (MQ=255)
            caTGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGc                                                                                                                       >  5:330430/1‑148 (MQ=255)
                   ggtgTGCAGCAGTGGCTCGGATCCGCTTTCTCTGAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGCCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCt                                                                                                               <  7:326661/149‑1 (MQ=255)
                    gtgtGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTg                                                                                                              >  8:303127/1‑149 (MQ=255)
                      gtgCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTgg                                                                                                             >  5:312294/1‑148 (MQ=255)
                       tgCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGaa                                                                                                           <  8:256501/149‑1 (MQ=255)
                        gcagcaGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACCACCGTGGTGATGGTGGTGATca                                                                                                                  >  2:149420/1‑140 (MQ=39)
                        gcagcaGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACCACCGTGGTGATGGTGGTGATca                                                                                                                  <  1:149420/141‑2 (MQ=39)
                         cagcagTGGCTCGGATCCGCTTTCACTCAACAAGCTTCTGGCCGGTAAGTGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAgc                                                                                                         <  8:140264/149‑1 (MQ=255)
                           gcagTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAgcgc                                                                                                       <  4:152509/149‑1 (MQ=255)
                                 gCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCCCCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACggtggtg                                                                                                                                  <  6:9855/116‑1 (MQ=255)
                                     ggATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGc                                                                                             <  7:303127/149‑1 (MQ=255)
                                        tCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGa                                                                                          <  4:224578/149‑1 (MQ=255)
                                          cGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGAcc                                                                                        >  4:141244/1‑149 (MQ=255)
                                               tCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTc                                                                                     <  6:312294/147‑1 (MQ=255)
                                               tCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCAGGAAGCGCAACCACTCGCAGACCTTc                                                                                     <  1:234955/147‑1 (MQ=255)
                                               tCACTCAACTCGCTTCGGCCCGGTAAGAGCGATATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCAGGAAGCGCAACCACTCGCAGACCTTc                                                                                     >  2:234955/1‑147 (MQ=255)
                                                 aCTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                  <  5:212460/148‑1 (MQ=255)
                                                 aCTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                  <  1:246535/148‑1 (MQ=255)
                                                  cTCAACTAGCATAGGCCCGGAAAGAGCGCTATGGAATTCAACACTCAAAACTTCATCGCATTGGCGCCGATGCAGAACACCACCATCAACACGGTGGAGGTCATGCTCGAGATCGCCTGGAAgc                                                                                                         >  4:310027/1‑124 (MQ=255)
                                                     aaCTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                  <  2:125257/144‑1 (MQ=255)
                                                     aaCTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                  >  1:125257/1‑144 (MQ=255)
                                                      aCTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAgcgc                                                                                                       >  4:33879/1‑122 (MQ=255)
                                                        tCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCAc                                                                          >  6:91827/1‑149 (MQ=255)
                                                        tCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCAc                                                                          >  5:103394/1‑149 (MQ=255)
                                                          gCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTc                                                                                               >  6:89988/1‑126 (MQ=255)
                                                          gCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTc                                                                                               <  5:89988/126‑1 (MQ=255)
                                                           cTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGt                                                                       <  4:26955/149‑1 (MQ=255)
                                                                gCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTg                                                                                                              <  6:58152/105‑1 (MQ=255)
                                                                gCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTg                                                                                                              >  5:58152/1‑105 (MQ=255)
                                                                  ccGGTAAGAGCGCTATGGAATTCACAACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACAATCACAACGGTGGTGGTGATGATGGCGATCGCCTGGAAGAGCAACCACTCGCAGACCTTCCTGCTGTCAAACGAGGGcat                                                                 >  5:216271/1‑146 (MQ=255)
                                                                     gTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAAc                                                             <  7:138081/149‑1 (MQ=255)
                                                                      tAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAAcc                                                            <  5:91827/149‑1 (MQ=255)
                                                                                  ggAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGt                                                >  6:278101/1‑149 (MQ=255)
                                                                                         accacTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCt                                         >  7:76821/1‑149 (MQ=255)
                                                                                          ccacTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTg                                        >  7:220670/1‑149 (MQ=255)
                                                                                             cTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCg                                     <  3:141244/149‑1 (MQ=255)
                                                                                                aaCACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGgcg                                  >  2:122744/1‑149 (MQ=255)
                                                                                                aaCACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGgcg                                  >  8:316090/1‑149 (MQ=255)
                                                                                                     ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGc                                                                   >  4:280690/1‑111 (MQ=255)
                                                                                                     ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGc                                                                   <  3:280690/111‑1 (MQ=255)
                                                                                                     ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTg                               >  4:245801/1‑147 (MQ=255)
                                                                                                       cATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAg                            <  8:220670/148‑1 (MQ=255)
                                                                                                         tagCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCAGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTc                         >  1:118610/3‑149 (MQ=255)
                                                                                                         tCGCATTGGCGCCGATGCTGATCACCACCATCACCATGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTc                         >  8:129027/1‑149 (MQ=255)
                                                                                                                 gcgcCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCt                 <  8:76821/149‑1 (MQ=255)
                                                                                                                            gATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg            <  3:35857/143‑1 (MQ=255)
                                                                                                                            gATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg            >  4:35857/1‑143 (MQ=255)
                                                                                                                              tCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCg    >  4:61619/1‑149 (MQ=255)
                                                                                                                                accaccAACACCACGCTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTCCAGACCACCGTGGGCCAGAACCTGGCCCTCCTCAAGCTCCTGCCCGCGCTGAAGGTCGCGCCACTTGCGGTCACATCGCt  >  8:123989/1‑149 (MQ=255)
                                                                                                                                 ccagcaTCACCACCACCGTGGTGATGGTGGTGATCGCCTGGACGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg            <  4:71304/138‑1 (MQ=38)
                                                                                                                                 ccagcaTCACCACCACCGTGGTGATGGTGGTGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg            >  3:71304/1‑138 (MQ=38)
                                                                                                                                   accaTCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTgg               >  3:181682/1‑133 (MQ=255)
                                                                                                                                   accaTCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTgg               <  4:181682/133‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                      
CTCTGCCGCCACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCT  >  NC_002947/4669589‑4669865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑