Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,669,744 C→G 7.1% L26V (CTG→GTG)  nuoN → NADH‑quinone oxidoreductase subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,669,7440CG 7.1% 173.0 / 2.8 56L26V (CTG→GTG) nuoNNADH‑quinone oxidoreductase subunit N
Reads supporting (aligned to +/- strand):  ref base C (26/25);  new base G (2/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

CACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCT  >  NC_002947/4669598‑4669888
                                                                                                                                                  |                                                                                                                                                
caccaTGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTg                                                                                                                                                <  5:28045/149‑1 (MQ=255)
   caTGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGc                                                                                                                                              >  5:330430/1‑148 (MQ=255)
          ggtgTGCAGCAGTGGCTCGGATCCGCTTTCTCTGAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGCCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCt                                                                                                                                      <  7:326661/149‑1 (MQ=255)
           gtgtGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTg                                                                                                                                     >  8:303127/1‑149 (MQ=255)
             gtgCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTgg                                                                                                                                    >  5:312294/1‑148 (MQ=255)
              tgCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGaa                                                                                                                                  <  8:256501/149‑1 (MQ=255)
               gcagcaGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACCACCGTGGTGATGGTGGTGATca                                                                                                                                         <  1:149420/141‑2 (MQ=39)
               gcagcaGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACCACCGTGGTGATGGTGGTGATca                                                                                                                                         >  2:149420/1‑140 (MQ=39)
                cagcagTGGCTCGGATCCGCTTTCACTCAACAAGCTTCTGGCCGGTAAGTGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAgc                                                                                                                                <  8:140264/149‑1 (MQ=255)
                  gcagTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAgcgc                                                                                                                              <  4:152509/149‑1 (MQ=255)
                            ggATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGc                                                                                                                    <  7:303127/149‑1 (MQ=255)
                               tCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGa                                                                                                                 <  4:224578/149‑1 (MQ=255)
                                 cGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGAcc                                                                                                               >  4:141244/1‑149 (MQ=255)
                                      tCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTc                                                                                                            <  6:312294/147‑1 (MQ=255)
                                      tCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCAGGAAGCGCAACCACTCGCAGACCTTc                                                                                                            <  1:234955/147‑1 (MQ=255)
                                      tCACTCAACTCGCTTCGGCCCGGTAAGAGCGATATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCAGGAAGCGCAACCACTCGCAGACCTTc                                                                                                            >  2:234955/1‑147 (MQ=255)
                                        aCTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                                         <  5:212460/148‑1 (MQ=255)
                                        aCTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                                         <  1:246535/148‑1 (MQ=255)
                                         cTCAACTAGCATAGGCCCGGAAAGAGCGCTATGGAATTCAACACTCAAAACTTCATCGCATTGGCGCCGATGCAGAACACCACCATCAACACGGTGGAGGTCATGCTCGAGATCGCCTGGAAgc                                                                                                                                >  4:310027/1‑124 (MQ=255)
                                            aaCTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                                         >  1:125257/1‑144 (MQ=255)
                                            aaCTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCctg                                                                                                         <  2:125257/144‑1 (MQ=255)
                                             aCTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAgcgc                                                                                                                              >  4:33879/1‑122 (MQ=255)
                                               tCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCAc                                                                                                 >  6:91827/1‑149 (MQ=255)
                                               tCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCAc                                                                                                 >  5:103394/1‑149 (MQ=255)
                                                 gCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTc                                                                                                                      >  6:89988/1‑126 (MQ=255)
                                                 gCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTc                                                                                                                      <  5:89988/126‑1 (MQ=255)
                                                  cTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGt                                                                                              <  4:26955/149‑1 (MQ=255)
                                                       gCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTg                                                                                                                                     <  6:58152/105‑1 (MQ=255)
                                                       gCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTg                                                                                                                                     >  5:58152/1‑105 (MQ=255)
                                                         ccGGTAAGAGCGCTATGGAATTCACAACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACAATCACAACGGTGGTGGTGATGATGGCGATCGCCTGGAAGAGCAACCACTCGCAGACCTTCCTGCTGTCAAACGAGGGcat                                                                                        >  5:216271/1‑146 (MQ=255)
                                                            gTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAAc                                                                                    <  7:138081/149‑1 (MQ=255)
                                                             tAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAAcc                                                                                   <  5:91827/149‑1 (MQ=255)
                                                                         ggAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGt                                                                       >  6:278101/1‑149 (MQ=255)
                                                                                accacTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCt                                                                >  7:76821/1‑149 (MQ=255)
                                                                                 ccacTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTg                                                               >  7:220670/1‑149 (MQ=255)
                                                                                    cTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCg                                                            <  3:141244/149‑1 (MQ=255)
                                                                                       aaCACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGgcg                                                         >  2:122744/1‑149 (MQ=255)
                                                                                       aaCACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGgcg                                                         >  8:316090/1‑149 (MQ=255)
                                                                                            ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGc                                                                                          >  4:280690/1‑111 (MQ=255)
                                                                                            ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGc                                                                                          <  3:280690/111‑1 (MQ=255)
                                                                                            ttCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTg                                                      >  4:245801/1‑147 (MQ=255)
                                                                                              cATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAg                                                   <  8:220670/148‑1 (MQ=255)
                                                                                                tagCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCAGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTc                                                >  1:118610/3‑149 (MQ=255)
                                                                                                tCGCATTGGCGCCGATGCTGATCACCACCATCACCATGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTc                                                >  8:129027/1‑149 (MQ=255)
                                                                                                        gcgcCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCt                                        <  8:76821/149‑1 (MQ=255)
                                                                                                                   gATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg                                   <  3:35857/143‑1 (MQ=255)
                                                                                                                   gATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg                                   >  4:35857/1‑143 (MQ=255)
                                                                                                                     tCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCg                           >  4:61619/1‑149 (MQ=255)
                                                                                                                       accaccAACACCACGCTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTCCAGACCACCGTGGGCCAGAACCTGGCCCTCCTCAAGCTCCTGCCCGCGCTGAAGGTCGCGCCACTTGCGGTCACATCGCt                         >  8:123989/1‑149 (MQ=255)
                                                                                                                        ccagcaTCACCACCACCGTGGTGATGGTGGTGATCGCCTGGACGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg                                   <  4:71304/138‑1 (MQ=38)
                                                                                                                        ccagcaTCACCACCACCGTGGTGATGGTGGTGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCgg                                   >  3:71304/1‑138 (MQ=38)
                                                                                                                          accaTCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTgg                                      >  3:181682/1‑133 (MQ=255)
                                                                                                                          accaTCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTgg                                      <  4:181682/133‑1 (MQ=255)
                                                                                                                                              gATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCg              >  3:31247/1‑137 (MQ=255)
                                                                                                                                              gATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCg              <  4:31247/137‑1 (MQ=255)
                                                                                                                                               aTGCTGGCGATCGCCGGGTAGCGCTAGCACTCGCAGAGCTTCCTGCTGTCCACGGTGTGCCTGAACCTGGCCCTGGTGTCGCTCCTGCCGGCGCTGAAGGTCGGGCCGCTGGCGGTGTCATCGCTGTTCTCCATCGAGAAGTTCgcct  <  7:123989/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CACCATGAGTGGTGTGCAGCAGTGGCTCGGATCCGCTTTCACTCAACTCGCTTCGGCCCGGTAAGAGCGCTATGGAATTCACCACTCAACACTTCATCGCATTGGCGCCGATGCTGATCACCACCATCACCACGGTGGTGGTGATGCTGGCGATCGCCTGGAAGCGCAACCACTCGCAGACCTTCCTGCTGTCCACCGTGGGCCTGAACCTGGCCCTGCTGTCGCTCCTGCCGGCGCTGAAGGTCGCGCCGCTGGCGGTCACATCGCTGATCACCATCGACAAGTTCGCCT  >  NC_002947/4669598‑4669888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑