Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,731,128	C→T	8.3%	V106I (GTT→ATT) ‡	lpdG ←	dihydrolipoyl dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,731,128	0	C	T	8.3%	169.8 / 4.1	60	V106I (GTT→ATT) ‡	lpdG	dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (30/25);  new base T (2/3);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

GCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCTTGGAA  >  NC_002947/4730984‑4731276                                                                                                                                                 |                                                                                                                                                     gcgAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCa                                                                                                                                                  >  5:282599/1‑149 (MQ=255)       cGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCGTTGCCAccctgt                                                                                                                                            <  1:176999/149‑5 (MQ=255)       cGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTc                                                                                                                                            <  3:150061/149‑1 (MQ=255)        ggAAGCCAGGATCACGTTCTCGGCTTCGATGACTTTGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCa                                                                                                                                           <  1:57022/149‑1 (MQ=255)         gAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGt                                                                                                                                             >  3:52027/1‑146 (MQ=255)           aGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCa                                                                                                                                           <  4:52027/146‑1 (MQ=255)                 gATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCGTTGCCAccctgt                                                                                                                                            <  4:93395/139‑5 (MQ=255)                 gATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCGTTGCCAccctgt                                                                                                                                            >  3:93395/1‑135 (MQ=255)                 gATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGa                                                                                                                                  <  2:221335/149‑1 (MQ=255)                 gATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCATGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGa                                                                                                                                  >  5:36727/1‑149 (MQ=255)                       gTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCGTTGCCAccctgt                                                                                                                                            >  4:121591/1‑129 (MQ=255)                       gTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCGTTGCCAccctgt                                                                                                                                            <  3:121591/133‑5 (MQ=255)                                      gACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCaa                                                                                                             >  2:203058/1‑149 (MQ=255)                                        cTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAAc                                                                                                            >  2:98818/1‑148 (MQ=255)                                         ttCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCCCCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAAcc                                                                                                           >  8:72253/1‑148 (MQ=255)                                         ttCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGAt                                                                                                                              <  8:219924/129‑1 (MQ=255)                                         ttCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGAt                                                                                                                              >  7:219924/1‑129 (MQ=255)                                         ttCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCa                                                                                                          >  2:175313/1‑149 (MQ=255)                                         ttCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCa                                                                                                          >  4:300076/1‑149 (MQ=255)                                         ttCGGTGGTGCCGTCAGCCTTGGTGACTTAGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTTACGATGCCGGCCTTGCGGCCAACCa                                                                                                          >  8:58938/1‑149 (MQ=255)                                             gtggtgCCGTCAGCCTTGGGGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATcgc                                                                                                      <  3:300076/149‑1 (MQ=255)                                                        aGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTcc                                                                                            <  8:27653/148‑1 (MQ=255)                                                              ggTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCtt                                                                                       <  6:282599/147‑1 (MQ=255)                                                               gTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCa                                                                                     <  4:21431/148‑1 (MQ=255)                                                               gTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCAACGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCAc                                                                                    >  4:212637/1‑149 (MQ=255)                                                               gTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGCGTGCCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCa                                                                                     <  8:273303/148‑1 (MQ=255)                                                                tGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACt                                                                                   <  6:36727/149‑1 (MQ=255)                                                                 gACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCa                                                                                                              >  1:38954/1‑121 (MQ=255)                                                                 gACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCa                                                                                                              <  2:38954/121‑1 (MQ=255)                                                                 gACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACtt                                                                                  >  1:152188/1‑149 (MQ=255)                                                                    ttCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGc                                                                               >  7:136572/1‑149 (MQ=255)                                                                    ttCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGc                                                                               >  1:133123/1‑149 (MQ=255)                                                                         cTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTg                                                                          <  2:133123/149‑1 (MQ=255)                                                                         cTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTg                                                                          <  7:113037/149‑1 (MQ=255)                                                                            tCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTgg                                                                         <  2:152188/147‑1 (MQ=255)                                                                            tCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTgg                                                                         <  3:160244/147‑1 (MQ=255)                                                                                           gcTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATTCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTg                                                        <  7:58938/149‑1 (MQ=255)                                                                                            cTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCgcggc                                                                                                   >  2:110853/1‑105 (MQ=255)                                                                                            cTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCgcggc                                                                                                   <  1:110853/105‑1 (MQ=255)                                                                                              tGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAg                                                     >  6:171261/1‑149 (MQ=255)                                                                                               gCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGa                                                                                                 >  4:133123/1‑104 (MQ=255)                                                                                               gCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGa                                                                                                 <  3:133123/104‑1 (MQ=255)                                                                                                ccGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGct                                                   >  7:113677/1‑149 (MQ=255)                                                                                                       ccTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTg                                            >  1:216552/1‑149 (MQ=255)                                                                                                       ccTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTg                                            >  3:199683/1‑149 (MQ=255)                                                                                                        cTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCttggc                                           >  6:219070/1‑146 (MQ=255)                                                                                                        cTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCttatg                                           >  8:261534/1‑146 (MQ=255)                                                                                                                aaGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTg                                   >  8:182288/1‑149 (MQ=255)                                                                                                                  gTGAAGCCGTTGGCCTTGAACAGGGTTGAAACGCCACCTGTCAGGTTCTTGACGATGCCGGGCTTGCGGGAATCCATCGCGGCGACGTCCATCTTAACTTCGCCCGTGGAGATACCGTGGACGTTGAATCTCTCTTTGGCTTCCTTGTa                                 <  8:113677/149‑1 (MQ=255)                                                                                                                           ttGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGa                        <  1:175313/149‑1 (MQ=255)                                                                                                                                 ttGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTc                  >  7:166506/1‑149 (MQ=255)                                                                                                                                   gAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTc                  >  8:229188/1‑147 (MQ=255)                                                                                                                                    aaCAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCca                <  3:212637/148‑1 (MQ=255)                                                                                                                                     aCAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTg                                                               >  7:208090/1‑100 (MQ=255)                                                                                                                                     aCAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTg                                                               <  8:208090/100‑1 (MQ=255)                                                                                                                                        gggTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCagcagc           >  7:242377/1‑149 (MQ=255)                                                                                                                                          gTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAgcgc         >  8:169259/1‑149 (MQ=255)                                                                                                                                             gCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCTTCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCtt      >  6:324967/1‑149 (MQ=255)                                                                                                                                             gCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCtt      <  4:199683/149‑1 (MQ=255)                                                                                                                                                aCGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCTTCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCTTgga   <  5:324967/149‑1 (MQ=255)                                                                                                                                                 cGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCTTggaa  <  7:182288/149‑1 (MQ=255)                                                                                                                                                 cGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCTTgg    >  7:95252/1‑147 (MQ=255)                                                                                                                                                 |                                                                                                                                                     GCGAGCCGGAAGCCAGGATCACGTTCTCGGCTTCGATGACTTCGGTGGTGCCGTCAGCCTTGGTGACTTCGACTTTCTTGCCGGCCAGCAGCTTGCCGTGGCCCTGGATCGAAGTGACGCCGTTGGCCTTGAACAGGGTGGCAACGCCACCGGTCAGGTTCTTGACGATGCCGGCCTTGCGGCCAACCATCGCGGCGACGTCCATCTTCACTTCGCCCGTGGAGATACCGTGGACGTTGAAGCTCTCTTTGGCTTCCTTGTACTTCCAGGAGCTGTCCAGCAGCGCCTTGGAA  >  NC_002947/4730984‑4731276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑