| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,782,608 | A→G | 8.5% | L1569P (CTC→CCC) | pvdJ ← | non‑ribosomal peptide synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,782,608 | 0 | A | G | 8.5% | 161.1 / 5.1 | 59 | L1569P (CTC→CCC) | pvdJ | non‑ribosomal peptide synthetase |
| Reads supporting (aligned to +/- strand): ref base A (25/29); new base G (3/2); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 | |||||||||||
CACCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCAG > NC_002947/4782465‑4782755 | ccccacggTCCCAGCAACACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGGGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAg < 4:29352/143‑1 (MQ=255) aCCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAg < 8:43694/148‑1 (MQ=255) aCCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAg < 6:75060/148‑1 (MQ=255) ccAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCTCGAACCGGGTTCGGGCatctat < 6:34852/149‑6 (MQ=255) ccAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGgtg > 7:289949/1‑149 (MQ=255) ccAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGgtg > 6:1037/1‑149 (MQ=255) cAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGgtgt < 7:236150/149‑1 (MQ=255) gTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCa > 3:66889/1‑149 (MQ=255) ccAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCAt > 1:178325/1‑147 (MQ=255) gCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATcag > 1:141460/1‑147 (MQ=255) gAGGTGCGCAGCACGTCATGGCGCTCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGTTGTGCATCAGCAGCCCTTc < 8:78933/149‑1 (MQ=255) gcagcaCGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGgtgt < 7:311080/126‑1 (MQ=255) gcagcaCGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGgtgt > 8:311080/1‑126 (MQ=255) cGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGAc < 8:289949/149‑1 (MQ=255) gcgcgcAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGa < 7:182012/104‑1 (MQ=255) gcgcgcAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGa > 8:182012/1‑104 (MQ=255) cgcgcAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATa < 7:335759/149‑1 (MQ=255) aCCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAg > 2:279874/1‑99 (MQ=255) aCCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAg < 1:279874/99‑1 (MQ=255) cGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGa > 5:222974/1‑149 (MQ=255) cGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGa > 8:172876/1‑149 (MQ=255) cAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCt > 6:322334/1‑112 (MQ=255) gCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATcgcgcgc > 6:247536/1‑149 (MQ=255) cTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCagcagc < 8:323969/101‑1 (MQ=255) cTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCagcagc > 7:323969/1‑101 (MQ=255) cTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATcgcgcgcg < 4:66889/149‑1 (MQ=255) gCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCAt < 1:157203/90‑1 (MQ=255) gCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCAt > 2:157203/1‑90 (MQ=255) cTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCAc > 5:348471/1‑149 (MQ=255) cGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGAGCGGCCACCGGCAGGCGa > 4:313760/1‑149 (MQ=255) aTCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCa < 2:158983/149‑1 (MQ=255) tCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAg < 6:348471/149‑1 (MQ=255) aaTGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGTGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGtt < 2:178325/149‑1 (MQ=255) aaTGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGtt < 7:320331/149‑1 (MQ=255) aaTGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGtt > 3:123407/1‑149 (MQ=255) cGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTtgctgc < 4:123407/149‑1 (MQ=255) gTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTgctgct < 5:247536/149‑1 (MQ=255) gTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGc > 8:264567/1‑149 (MQ=255) aCGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAg < 6:222974/149‑1 (MQ=255) ggTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCg < 5:1037/149‑1 (MQ=255) gCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGc > 4:285752/1‑147 (MQ=255) aaTACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGt < 3:313760/149‑1 (MQ=255) aTACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGCTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTg > 3:58407/1‑149 (MQ=255) tACTGCATCCGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTgg < 6:193822/149‑1 (MQ=255) tACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTgg < 7:172876/149‑1 (MQ=255) gatagatGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCtt > 3:43111/1‑149 (MQ=255) accttGCTCGAACCGGGTTCGGGCATGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATc < 4:63004/103‑1 (MQ=255) accttGCTCGAACCGGGTTCGGGCATGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATc > 3:63004/5‑107 (MQ=255) cttGCTCGAACCGGGTTCGGGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCg > 8:141576/3‑149 (MQ=255) cttGCTCGAACCGGGTTCGGGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCCATTTCGGCCACAGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCg > 1:294789/3‑149 (MQ=255) ccGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATc > 6:341952/1‑100 (MQ=255) ccGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATc < 5:341952/100‑1 (MQ=255) aCCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCa > 1:130405/1‑113 (MQ=255) aCCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCa < 2:130405/113‑1 (MQ=255) aCCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCtt < 3:101495/149‑1 (MQ=255) ccGGTTCGAGCAAGGTGTGCATCAGCAGACCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCATGCGATCCATTTTCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTgc > 2:138809/1‑149 (MQ=255) gTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGc < 6:103604/115‑1 (MQ=255) gTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGc > 5:103604/1‑115 (MQ=255) gTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGc < 4:153744/115‑1 (MQ=255) gTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGc > 3:153744/1‑115 (MQ=255) gAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCAg < 4:58407/149‑1 (MQ=255) | CACCCAGGTCCCAGCAAAACGAGGTGCGCAGCACGTCATGGCGCGCAATGACCGCTTTCCAGGCTTCGCTGAACGCGCCGACATCAATGGCGTGGTCGACGCGGTAGCAATACTGCATCAGATAGATGCCCGAACCCGGTTCGAGCAAGGTGTGCATCAGCAGCCCTTCCTGCATCGGCGACAACGGATAGATGTCTTCGATATCGCGCGCGGCCACCGGCAGGCGATCCAGTTGCTGCTGGCTCAGCCCGGCCAGTGGGAAGTCGCTTGGCGTAATGCCTTGCGCCTCAG > NC_002947/4782465‑4782755 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |