Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,809,869 T→C 6.3% V475A (GTG→GCG)  dsbD‑II → thiol/disulfide interchange protein 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,809,8690TC 6.3% 180.4 / 2.4 63V475A (GTG→GCG) dsbD‑IIthiol/disulfide interchange protein 2
Reads supporting (aligned to +/- strand):  ref base T (33/26);  new base C (2/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

TGGCAGCCACTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGCCCGGCCAGCAAAGCCCTG  >  NC_002947/4809730‑4810017
                                                                                                                                           |                                                                                                                                                    
tGGCAGCCACTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGa                                                                                                                                             >  2:248115/1‑149 (MQ=255)
  gCAGCCACTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTCGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGac                                                                                                                                            >  4:307279/1‑148 (MQ=255)
   cAGCCACTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGacta                                                                                                                                          >  1:148302/1‑149 (MQ=255)
    aGCCACTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTACGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGactac                                                                                                                                         <  3:106986/149‑1 (MQ=255)
        aCTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATg                                                                                                                                     >  5:70517/1‑149 (MQ=255)
             gCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGAc                                                                                                                                <  1:219438/149‑1 (MQ=255)
                    cGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCg                                                                                                                         >  3:22302/1‑149 (MQ=255)
                            gCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTg                                                                                                                                                     >  6:109785/1‑113 (MQ=255)
                            gCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTg                                                                                                                                                     <  5:109785/113‑1 (MQ=255)
                            gCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTg                                                                                                                  >  1:66543/1‑148 (MQ=255)
                                  ccgcGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTc                                                                                                           <  3:307929/149‑1 (MQ=255)
                                    gcgACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCAt                                                                                                         <  1:210663/149‑1 (MQ=255)
                                    gcgACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCAt                                                                                                         >  2:36260/1‑149 (MQ=255)
                                        cAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGa                                                                                                      >  2:285832/1‑148 (MQ=255)
                                              cAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAg                                                                                               <  1:248115/149‑1 (MQ=255)
                                              cAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAg                                                                                               <  3:307279/149‑1 (MQ=255)
                                                    aaCACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTc                                                                                         >  8:95690/1‑149 (MQ=255)
                                                       acGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTTTTc                                                                                         <  1:102187/146‑1 (MQ=255)
                                                       acGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTTTTc                                                                                         >  2:102187/1‑146 (MQ=255)
                                                       acGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTcgca                                                                                      <  1:36260/149‑1 (MQ=255)
                                                                   gCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTa                                                                          >  8:27373/1‑149 (MQ=255)
                                                                    cTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTa                                                                          >  8:23492/1‑148 (MQ=255)
                                                                        gTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAgg                                                                      <  7:95690/148‑1 (MQ=255)
                                                                         tCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAggcc                                                                    >  3:27198/1‑149 (MQ=255)
                                                                          cACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAggccg                                                                   >  3:66597/1‑149 (MQ=255)
                                                                               tCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTg                                                              <  7:27373/149‑1 (MQ=255)
                                                                                cAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTgg                                                             >  4:260095/1‑149 (MQ=255)
                                                                                     gccCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCAt                                                                                                         <  8:225625/100‑1 (MQ=255)
                                                                                     gccCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCAt                                                                                                         >  7:225625/1‑100 (MQ=255)
                                                                                      ccccGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCg                                                       <  3:260095/149‑1 (MQ=255)
                                                                                        ccGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTa                                                     >  2:93179/1‑149 (MQ=255)
                                                                                        ccGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCATGACCGGCGTa                                                     >  1:302880/1‑149 (MQ=255)
                                                                                         cGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTa                                                     >  2:230667/1‑148 (MQ=255)
                                                                                          gAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACAACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTaca                                                   >  7:214458/1‑149 (MQ=255)
                                                                                           aaGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGc                                                        <  6:339882/143‑1 (MQ=255)
                                                                                           aaGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGc                                                        >  5:339882/1‑143 (MQ=255)
                                                                                                cTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACa                                                                                                >  7:44872/1‑98 (MQ=255)
                                                                                                cTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACa                                                                                                <  8:44872/98‑1 (MQ=255)
                                                                                                  gCAACATGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCtgctgc                                           >  1:327321/1‑149 (MQ=255)
                                                                                                     aCGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGcc                                        >  6:197958/1‑149 (MQ=255)
                                                                                                      cGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCt                                       <  4:27198/149‑1 (MQ=255)
                                                                                                      cGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCt                                       <  4:66597/149‑1 (MQ=255)
                                                                                                         gAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCg                                                                               <  2:338221/106‑1 (MQ=255)
                                                                                                         gAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCg                                                                               >  1:338221/1‑106 (MQ=255)
                                                                                                                 tGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGc                                                            >  2:45315/1‑117 (MQ=255)
                                                                                                                 tGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGc                                                            <  1:45315/117‑1 (MQ=255)
                                                                                                                  gCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGcc                                                           >  6:92145/1‑117 (MQ=255)
                                                                                                                   cGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGc                           <  4:22302/148‑1 (MQ=255)
                                                                                                                   cGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGc                           <  2:148302/148‑1 (MQ=255)
                                                                                                                      ccAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGa                        >  5:354802/1‑148 (MQ=255)
                                                                                                                        aaaGCCCGCGGCCAGTGGGCGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAAc                                                                                                 <  3:135890/73‑1 (MQ=255)
                                                                                                                        aaaGCCCGCGGCCAGTGGGCGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAAc                                                                                                 >  4:135890/1‑73 (MQ=255)
                                                                                                                        aaaGCCCGCGGCCAGTGGGCGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAAc                                                                                                 >  4:130673/1‑73 (MQ=255)
                                                                                                                        aaaGCCCGCGGCCAGTGGGCGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAAc                                                                                                 <  3:130673/73‑1 (MQ=255)
                                                                                                                         aaGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGc                    >  3:126276/1‑149 (MQ=255)
                                                                                                                          aGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGcc                   >  5:247492/1‑149 (MQ=255)
                                                                                                                            cccGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGCCCg                 <  6:70517/149‑1 (MQ=255)
                                                                                                                              cgcgGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTg                                <  8:115643/132‑1 (MQ=255)
                                                                                                                              cgcgGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTg                                >  7:115643/1‑132 (MQ=255)
                                                                                                                                    cAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGCCCGGCCAGCaa         <  2:327321/149‑1 (MQ=255)
                                                                                                                                     aGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGCCCGGCCAGCaaa        <  4:126276/149‑1 (MQ=255)
                                                                                                                                       tGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAggccggc                                                                 <  4:72015/90‑1 (MQ=255)
                                                                                                                                       tGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAggccggc                                                                 >  3:72015/1‑90 (MQ=255)
                                                                                                                                           tGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGAc                       >  6:72004/1‑128 (MQ=255)
                                                                                                                                           tGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGAc                       <  5:72004/128‑1 (MQ=255)
                                                                                                                                           tGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGCCCGGCCAGCAAAGCCctg  >  5:101037/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TGGCAGCCACTACGCCCGTTCGCCGGTGGCGTCGCCGCGACAGCCCCAAGCCAACACGCCGAAGACCGCTTCGTCACCGTCAGCCGCCCCGAAGACCTGCAACGTGAGCTGGATGCGGCCAAAGCCCGCGGCCAGTGGGTGATGCTCGACTACTATGCCGACTGGTGCGTGTCGTGCAAAGTCATGGAAAAACAGGTATTCGCACGCAGCGACGTACAGGCCGGCCTGGCCGGCGTACACCTGCTGCGCCTGGACGTGACGGCCGACAGCCCGGCCAGCAAAGCCCTG  >  NC_002947/4809730‑4810017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑