Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,812,185	A→G	14.6%	intergenic (+105/‑288)	PP_4238 → / → PP_5665	hypothetical protein/McbA family microcin B17‑like processing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,812,185	0	A	G	14.6%	93.9 / 8.5	41	intergenic (+105/‑288)	PP_4238/PP_5665	hypothetical protein/McbA family microcin B17‑like processing protein
Reads supporting (aligned to +/- strand):  ref base A (17/18);  new base G (4/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATTGGTGATTGGACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCATTAT  >  NC_002947/4812039‑4812329                                                                                                                                                   |                                                                                                                                                 acaTTGGTGATTGGACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCaaa                                                                                                                                                 >  4:319596/1‑148 (MQ=255)         gATTGGACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCAt                                                                                                                                        >  3:202485/1‑149 (MQ=255)                cGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTcgcg                                                                                                                                 <  1:137663/149‑1 (MQ=255)                 gCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCgcgc                                                                                                                                >  8:12456/1‑149 (MQ=255)                   aGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTTTGACATCAGc                                                                                                                                          <  1:279173/137‑1 (MQ=255)                   aGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTTTGACATCAGc                                                                                                                                          >  2:279173/1‑137 (MQ=255)                               cGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAAc                                                                                                                   <  8:13563/148‑1 (MQ=255)                               cGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAAc                                                                                                                   <  6:286187/148‑1 (MQ=255)                                                     atcaatcTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGaa                                                                                            <  5:289793/149‑1 (MQ=255)                                                            tcACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATGTCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACaaa                                                                                               >  1:273219/1‑139 (MQ=255)                                                            tcACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCtta                                                                                                                          <  5:150505/112‑1 (MQ=255)                                                            tcACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCtta                                                                                                                          >  6:150505/1‑112 (MQ=255)                                                            tcACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACaaa                                                                                               <  2:273219/139‑1 (MQ=255)                                                            tcACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTcac                                                                                     <  3:319596/149‑1 (MQ=255)                                                              aCGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCacac                                                                                   <  4:202485/149‑1 (MQ=255)                                                               cGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACg                                                                                  >  2:251604/1‑149 (MQ=255)                                                                    cGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTTTGACATCAGCa                                                                                                                                         <  6:163989/89‑1 (MQ=39)                                                                    cGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTTTGACATCAGCa                                                                                                                                         >  5:163989/1‑89 (MQ=39)                                                                    cGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcac                                                                             >  6:20751/1‑149 (MQ=255)                                                                              aTCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCtt                                                                   >  3:180435/1‑149 (MQ=255)                                                                                taccACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTc                                                                 <  5:293445/147‑1 (MQ=255)                                                                                 cccACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCATACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCg                                                                >  1:137632/1‑149 (MQ=255)                                                                                    aCGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCAc                                                             >  8:74637/1‑149 (MQ=255)                                                                                           tCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGaa                                                      >  2:212127/1‑149 (MQ=255)                                                                                               aCCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCATACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAg                                                  <  2:137632/149‑1 (MQ=255)                                                                                                   tGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGc                                              >  3:126242/1‑149 (MQ=255)                                                                                                        cTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGcttctt                                         <  5:20751/149‑1 (MQ=255)                                                                                                         taaaaaTATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATAAGAATGCTCGCGCCCCTTATTAAAACTTCACACCGAGCATAACAAAGAACACTca                                                                                      >  6:87123/3‑103 (MQ=255)                                                                                                         tCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTa                                        <  7:12456/149‑1 (MQ=255)                                                                                                           aaaaTATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACa                                      >  6:247877/1‑149 (MQ=255)                                                                                                           aaaaTATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACa                                      >  5:66886/1‑149 (MQ=255)                                                                                                           aaaaTATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCAAACCACAGAGATTTCGCACGCCAGAATCAGAAGCTTCTTAc                                       >  1:117544/1‑148 (MQ=255)                                                                                                                     gAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTcacc                                                                            <  4:240807/101‑1 (MQ=255)                                                                                                                     gAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATaa                             <  5:247877/148‑1 (MQ=255)                                                                                                                     gAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGATcacc                                                                            >  3:240807/1‑101 (MQ=255)                                                                                                                           aTTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCTCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCAc                      <  1:251604/149‑1 (MQ=255)                                                                                                                                      cATGCTGATGTTTGACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTaa            >  1:181615/1‑148 (MQ=255)                                                                                                                                       aTGCTGATGTTTGACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTaaa           >  3:346451/1‑148 (MQ=255)                                                                                                                                             aTGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCa      <  4:126242/147‑1 (MQ=255)                                                                                                                                             aTGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCa      <  7:74637/147‑1 (MQ=255)                                                                                                                                             aTGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCAt     <  1:212127/148‑1 (MQ=255)                                                                                                                                                tCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCATTAt  >  2:283551/1‑148 (MQ=255)                                                                                                                                                  aaaCATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCATTAt  >  6:146566/1‑146 (MQ=255)                                                                                                                                                  aaaCATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCATTAt  >  8:182212/1‑146 (MQ=255)                                                                                                                                                   |                                                                                                                                                 ACATTGGTGATTGGACGCAGGCCGTTCTTTCGCAAGCAGTAAATCTCCCGAAATCAATCTCACGACCCGGCAAATCTATCCCCACGCAGGTCTAACCCTGCAACTCAAAATATCTAGAAACAATTCTTCGGAGCATGCTGATGTCAAACATCAGCATGCTCGCGCCCCTTATTAAAACTTCACACCGCGCATAACAAAGAACACTCACACGCTCACCACAGAGCTTTCGCACGCCAGAATCAGAAGCTTCTTACATAAATATAAGAAGCACTGAAAACTAAAAACCATTAT  >  NC_002947/4812039‑4812329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑