Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,886,168	A→T	14.3%	intergenic (+47/+76)	PP_4295 → / ← PP_4296	TetR family transcriptional regulator/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,886,168	0	A	T	14.3%	62.5 / 4.6	28	intergenic (+47/+76)	PP_4295/PP_4296	TetR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/13);  new base T (2/2);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01

GCGGTGACCGGTAAGGCCAAGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTC  >  NC_002947/4886020‑4886312                                                                                                                                                     |                                                                                                                                                 gCGGTGACCGGTAAGGCCAAGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATca                                                                                                                                                  >  5:257419/1‑149 (MQ=255)  cGGTGACCGGTAAGGCCATGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCATCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATca                                                                                                                                                  <  2:180788/148‑1 (MQ=255)           gTAAGGCCAAGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCt                                                                                                                                        >  2:153481/1‑149 (MQ=255)              aGGCCAAGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGaa                                                                                                                                     <  2:200191/149‑1 (MQ=255)                  cAAGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGtt                                                                                                                                 <  4:59538/149‑1 (MQ=255)                     gCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTtgtg                                                                                                                              <  1:153481/149‑1 (MQ=255)                       tGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACACGATCACCACATGCCTGAAGGTTGTGGa                                                                                                                            <  1:16067/149‑1 (MQ=255)                            gACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTccc                                                                                                                       >  7:260735/1‑149 (MQ=255)                                gTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCtgtg                                                                                                                   <  1:205957/149‑1 (MQ=255)                                gTGATTAGGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCtgtg                                                                                                                   <  8:128364/149‑1 (MQ=255)                                      aTGACGCGGCGGCCGAGACCATCATTCGTATTGTCTTGAAAGGTTGTGAGCCTGAGTCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGc                                                                                                              <  8:99742/148‑1 (MQ=255)                                       tGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGa                                                                                                                            >  5:56511/1‑133 (MQ=255)                                       tGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGa                                                                                                                            <  6:56511/133‑1 (MQ=255)                                         aCGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCggg                                                                                                           <  6:257419/148‑1 (MQ=255)                                           gcggcgGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAAACATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTtgt                                                                                                                               <  3:184267/126‑1 (MQ=255)                                           gcggcgGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAAACATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTtgt                                                                                                                               >  4:184267/1‑126 (MQ=255)                                            cggcggCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTg                                                                                                       <  3:28623/149‑1 (MQ=17)                                               cggcCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGccc                                                                                                    <  8:260735/149‑1 (MQ=255)                                                         catcatTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGa                                                                                                                            >  1:173319/1‑115 (MQ=255)                                                         catcatTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGa                                                                                                                            <  2:173319/115‑1 (MQ=255)                                                                                   gtgAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTACCACAGGATCACCACATGCCTGAAGGGTGTGGAGTCCCTGTGGGAGCGCGCTTGCCCGCGAATGGGCCAGAAGAGGCAATAGAGATTTAGGcc                                                                >  5:307782/1‑149 (MQ=255)                                                                                                                     ccATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCAcctcct                              >  5:76713/1‑149 (MQ=255)                                                                                                                       aTTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGCATGGGGCAGAACAGGCAATAGAGCTTTAGGCCGCGACCCCGGCATCTGCCCGCCGCCCCCcctccgc                             >  2:310924/1‑146 (MQ=38)                                                                                                                                 aaGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGa                  >  7:210794/1‑149 (MQ=255)                                                                                                                                     ccGCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCg               >  5:181830/1‑148 (MQ=18)                                                                                                                                     ccGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCg               >  6:54998/1‑148 (MQ=255)                                                                                                                                       gCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTGCCCGCGAAT‑GGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCg                        >  7:30873/1‑136 (MQ=17)                                                                                                                                       gCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTGCCCGCGAAT‑GGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCg                        <  8:30873/136‑1 (MQ=17)                                                                                                                                                 gATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCtcgtc  <  1:310924/149‑1 (MQ=255)                                                                                                                                                  aTCACAACATGCCTGAAGGTTGTGGAGTCACTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGAATTAGACCGCTACCCCg                                                       >  7:226981/1‑95 (MQ=38)                                                                                                                                                     |                                                                                                                                                 GCGGTGACCGGTAAGGCCAAGCTGGCCGACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTC  >  NC_002947/4886020‑4886312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑