| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,886,192 | T→A | 16.7% | intergenic (+71/+52) | PP_4295 → / ← PP_4296 | TetR family transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,886,192 | 0 | T | A | 16.7% | 53.2 / 4.8 | 24 | intergenic (+71/+52) | PP_4295/PP_4296 | TetR family transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/10); new base A (2/2); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.49e-01 | |||||||||||
GACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTA > NC_002947/4886047‑4886325 | gACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTccc > 7:260735/1‑149 (MQ=255) gTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCtgtg < 1:205957/149‑1 (MQ=255) gTGATTAGGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCtgtg < 8:128364/149‑1 (MQ=255) aTGACGCGGCGGCCGAGACCATCATTCGTATTGTCTTGAAAGGTTGTGAGCCTGAGTCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGc < 8:99742/148‑1 (MQ=255) aCGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCggg < 6:257419/148‑1 (MQ=255) cggcggCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTg < 3:28623/149‑1 (MQ=17) cggcCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGccc < 8:260735/149‑1 (MQ=255) gtgAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTACCACAGGATCACCACATGCCTGAAGGGTGTGGAGTCCCTGTGGGAGCGCGCTTGCCCGCGAATGGGCCAGAAGAGGCAATAGAGATTTAGGcc > 5:307782/1‑149 (MQ=255) ccATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCAcctcct > 5:76713/1‑149 (MQ=255) aTTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGCATGGGGCAGAACAGGCAATAGAGCTTTAGGCCGCGACCCCGGCATCTGCCCGCCGCCCCCcctccgc > 2:310924/1‑146 (MQ=38) aaGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGa > 7:210794/1‑149 (MQ=255) ccGCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCg > 5:181830/1‑148 (MQ=18) ccGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCg > 6:54998/1‑148 (MQ=255) gCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTGCCCGCGAAT‑GGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCg < 8:30873/136‑1 (MQ=17) gCTCCCACAGGGACTCCACAACCTTCAGGCATGTGGTGATCCTGTGGGAGCGGGCTTGCCCGCGAAT‑GGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCg > 7:30873/1‑136 (MQ=17) gATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCtcgtc < 1:310924/149‑1 (MQ=255) aTCACAACATGCCTGAAGGTTGTGGAGTCACTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGAATTAGACCGCTACCCCg > 7:226981/1‑95 (MQ=38) ccacATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTAccc < 6:283370/89‑1 (MQ=255) ccacATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTAccc > 5:283370/1‑89 (MQ=255) acaTGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATcc < 6:76713/149‑1 (MQ=255) aTGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGa > 2:179853/1‑149 (MQ=255) cTGAAGGTTTTGGAGTCCGTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTa < 6:307782/149‑1 (MQ=255) tGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCAc > 6:221428/1‑102 (MQ=255) tGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCAc < 5:221428/102‑1 (MQ=255) | GACAGTGATTATGACGCGGCGGCCGAGACCATCATTCGTATGGTCTTGAAAGGTTGTGAGCCTGAGGCAGCCTGAACCATCTCGAAGGGCCATTCGCGGGCAAGCCCGCTCCCACAGGATCACCACATGCCTGAAGGTTGTGGAGTCCCTGTGGGAGCGGGCTTGCCCGCGAATGGGCCAGAACAGGCAATAGAGATTTAGGCCGCTACCCCGGCATCTGCCCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTA > NC_002947/4886047‑4886325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |