Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,889,710 G→A 8.1% A2T (GCT→ACT)  glxR → 2‑hydroxy‑3‑oxopropionate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,889,7100GA 8.1% 138.3 / 2.2 49A2T (GCT→ACT) glxR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  ref base G (24/21);  new base A (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

GACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTG  >  NC_002947/4889567‑4889850
                                                                                                                                               |                                                                                                                                            
gACCCTGTGGTAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTaaa                                                                                                                                         <  5:18356/149‑1 (MQ=255)
              gggCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTAGCGGGCGAGCCCCCGCCCACAGGGGGCCAAAGCGTGCGTTAGATATAACAATTACAAAGAGGTTATTTGTCATGGCTAAAAACGGTTTGATCgg                                                                                                                           >  2:356978/1‑149 (MQ=37)
                      ccGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCAt                                                                                                                              <  6:188256/138‑1 (MQ=255)
                      ccGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCAt                                                                                                                              >  5:188256/1‑138 (MQ=255)
                      ccGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGca                                                                                                                   >  5:284394/1‑149 (MQ=255)
                         atggcacCGGCTTTGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGt                                                                                                                                   >  1:55738/5‑130 (MQ=255)
                         atggcacCGGCTTTGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGt                                                                                                                                   <  2:55738/126‑1 (MQ=39)
                            acacCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATgg                                                                                                             >  6:274030/1‑149 (MQ=255)
                              acCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATggg                                                                                                            >  1:53215/1‑148 (MQ=255)
                                     aaGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGccc                                                                                                     >  8:31451/1‑148 (MQ=255)
                                     aaGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGccc                                                                                                     <  7:31451/148‑1 (MQ=255)
                                                 tCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATTGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAAcc                                                                                        <  2:21072/149‑1 (MQ=255)
                                                 tCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAAcc                                                                                        >  4:75310/1‑149 (MQ=255)
                                                    aCCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTg                                                                                      >  6:316119/1‑148 (MQ=255)
                                                        aGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa                                                                                 <  3:75310/149‑1 (MQ=255)
                                                          ttGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAg                                                                                <  2:127786/148‑1 (MQ=255)
                                                                gCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGt                                                                          >  1:278504/1‑148 (MQ=255)
                                                                 cTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTca                                                                        >  4:208289/1‑149 (MQ=255)
                                                                 cTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTc                                                                         <  6:284394/148‑1 (MQ=255)
                                                                  ttCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTca                                                                        >  6:263748/1‑148 (MQ=255)
                                                                  ggagcgGGCTCGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTcac                                                                       >  2:62252/4‑149 (MQ=255)
                                                                     gcgGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCACAGAACCTGCAAAAGGCAGGTCACAGc                                                                    <  1:192289/149‑1 (MQ=255)
                                                                       gggCGAGCCCGCTCCCACATGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCt                                                                  <  7:57283/149‑1 (MQ=255)
                                                                         gCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGt                                                                <  3:208289/149‑1 (MQ=255)
                                                                         gCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGACTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCaaaa                                                                                 <  5:72219/132‑1 (MQ=255)
                                                                         gCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGACTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTCCaaaa                                                                                 >  6:77314/1‑132 (MQ=255)
                                                                         gCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGACTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTCCaaaa                                                                                 >  6:72219/1‑132 (MQ=255)
                                                                         gCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGACTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTCCaaaa                                                                                 <  5:77314/132‑1 (MQ=255)
                                                                               ccGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCCCAGAACCTGCAAAAGGCAGGTcaca                                                                      <  6:78792/137‑1 (MQ=255)
                                                                               ccGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCCCAGAACCTGCAAAAGGCAGGTcaca                                                                      >  5:78792/1‑137 (MQ=255)
                                                                               ccGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTATCATGGCTAAAATCGGTTTCATCAGCACCGGCATCATGGGCAAGCCCATGGCTCATAACCTGCAAAAGGCAGGTCACAGCCTGTTCAttt                                                          >  6:146043/1‑149 (MQ=255)
                                                                                 gCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTcc                                                        <  2:78667/149‑1 (MQ=255)
                                                                                 gCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTcc                                                        <  1:356978/149‑1 (MQ=255)
                                                                                   tCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccac                                                      >  5:17453/1‑149 (MQ=255)
                                                                                     ccACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccaccc                                                    <  2:53215/149‑1 (MQ=255)
                                                                                     ccACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccacc                                                     >  5:111798/1‑148 (MQ=255)
                                                                                      cacaGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccaccca                                                   >  1:69748/1‑149 (MQ=255)
                                                                                      cacaGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccaccca                                                   >  5:14147/1‑149 (MQ=255)
                                                                                       acaGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTccacccac                                                  <  5:274030/149‑1 (MQ=255)
                                                                                          gggggCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCt                                                                  <  8:320743/130‑1 (MQ=255)
                                                                                          gggggCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCt                                                                  >  7:320743/1‑130 (MQ=255)
                                                                                                             agaTATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGAc                             <  5:263748/148‑1 (MQ=255)
                                                                                                                      aaTTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTACATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCAGTTCCACCCACCACGACCCCGCGCCAGCCGACCTGATCGCTg                   >  8:205107/1‑149 (MQ=255)
                                                                                                                           cAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGt              <  6:111798/149‑1 (MQ=255)
                                                                                                                            aaaGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTg             >  2:197647/1‑149 (MQ=255)
                                                                                                                                  gTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCAttt                                                          <  2:72845/98‑1 (MQ=255)
                                                                                                                                  gTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCAttt                                                          >  1:72845/1‑98 (MQ=255)
                                                                                                                                  gTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTgg       >  4:2754/1‑149 (MQ=255)
                                                                                                                                        tctcATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTg  <  1:62252/148‑1 (MQ=255)
                                                                                                                                               gCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCg                      <  7:92782/121‑1 (MQ=255)
                                                                                                                                               gCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCg                      >  8:92782/1‑121 (MQ=255)
                                                                                                                                               |                                                                                                                                            
GACCCTGTGGGAGCGGGCTCGCCCGCGAACACCGGCAAAGCCGGTGCCATCCACCGAGTTGTCTGCTTCGCGGGCGAGCCCGCTCCCACAGGGGGCCAAAGCTTGCTTGAGATATCACAATTACAAAGAGGTAATTTCTCATGGCTAAAATCGGTTTCATCGGCACCGGCATCATGGGCAAGCCCATGGCTCAGAACCTGCAAAAGGCAGGTCACAGCCTGTTCATTTCCACCCACCACGACGCCGCGCCAGCCGACCTGATCGCTGCCGGTGCCGTGGCCCTG  >  NC_002947/4889567‑4889850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑