Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,991,234 A→C 8.0% intergenic (‑103/+126) PP_4399 ← / ← bkdR glutamine synthetase/Bkd operon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,991,2340AC 8.0% 151.4 / 4.6 50intergenic (‑103/+126)PP_4399/bkdRglutamine synthetase/Bkd operon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/25);  new base C (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGCCATCTGCAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTCAT  >  NC_002947/4991087‑4991377
                                                                                                                                                   |                                                                                                                                               
ggCCATCTGCAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAg                                                                                                                                                <  1:214215/149‑1 (MQ=255)
    aTCTGCAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTCCTCATGACGCATGTCCATGTTGTTGTAGGCt                                                                                                                                             >  3:74745/1‑148 (MQ=255)
         cAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGacaac                                                                                                                                       <  2:219316/149‑1 (MQ=255)
         cAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGac                                                                                                                                          <  4:86666/146‑1 (MQ=255)
           gacgacGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTCAGCCTacaacaa                                                                                                                                     >  6:60890/1‑149 (MQ=255)
              gacgGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTCAGCCTacaacaacaa                                                                                                                                  <  4:65080/149‑2 (MQ=255)
              gacgGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTCAGCCTacaacaacaa                                                                                                                                  <  5:60890/149‑2 (MQ=255)
               acgGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATa                                                                                                                                 <  8:103051/149‑1 (MQ=255)
                  gCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGc                                                                                                                              <  3:248980/149‑1 (MQ=255)
                     ttCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGc                                                                                                                           >  4:50525/1‑149 (MQ=255)
                                 ttCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTAGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAga                                                                                                                                               >  8:146987/1‑116 (MQ=255)
                                    ggcggTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCAGGTGTTCAAAatat                                                                                                            <  5:80793/149‑1 (MQ=255)
                                       ggTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATc                                                                                                           >  4:254724/1‑147 (MQ=255)
                                               ccTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTa                                                                                                 <  3:50525/149‑1 (MQ=255)
                                                            aaCGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCtgtg                                                                                    <  7:94973/149‑1 (MQ=255)
                                                             aCGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACAAAGGCTGCGGGTGTTCAAATTATCAagca                                                                                                      <  1:202253/130‑1 (MQ=255)
                                                             aCGCCGTTGAGGACGGGGGTGACGCATTCGATTTCCTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGATCAAAATATCAagca                                                                                                      >  2:202253/1‑130 (MQ=255)
                                                              cGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGcc                                                                                  <  7:314156/149‑1 (MQ=255)
                                                              cGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGcc                                                                                  >  8:314156/1‑149 (MQ=255)
                                                                     gAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCctct                                                                           >  1:1844/1‑149 (MQ=255)
                                                                        gTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         <  6:239513/100‑1 (MQ=255)
                                                                        gTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         >  5:239513/1‑100 (MQ=255)
                                                                           gggggTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCgg                                                                     <  3:274262/149‑1 (MQ=255)
                                                                           gggggTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCgg                                                                     <  8:275788/149‑1 (MQ=255)
                                                                                 gACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         >  3:255642/1‑91 (MQ=255)
                                                                                 gACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         <  4:255642/91‑1 (MQ=255)
                                                                                 gACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAAc                                                               >  1:48246/1‑149 (MQ=255)
                                                                                   cGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAAccc                                                             >  1:151730/1‑149 (MQ=255)
                                                                                    gCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCcg                                                            >  5:31685/1‑149 (MQ=255)
                                                                                       ttCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCgcgc                                                         >  7:24842/1‑149 (MQ=255)
                                                                                          gATTTCGTCGATGCCCTGGATCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTAACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTTCACCcgcgaaga                                                      <  4:74745/149‑5 (MQ=255)
                                                                                                   gATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGAt                                             <  2:107499/149‑1 (MQ=255)
                                                                                                   gATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGAt                                             <  2:1844/149‑1 (MQ=255)
                                                                                                    aTGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATg                                            >  5:208804/1‑149 (MQ=255)
                                                                                                    aTGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATg                                            <  3:254724/149‑1 (MQ=255)
                                                                                                    aTGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATg                                            <  6:31685/149‑1 (MQ=255)
                                                                                                        ccTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGt                                                 <  1:90427/140‑1 (MQ=255)
                                                                                                        ccTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGt                                                 >  2:90427/1‑140 (MQ=255)
                                                                                                        ccTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGAt                                        >  8:63840/1‑149 (MQ=255)
                                                                                                                 gAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTgg                                                                                         >  3:160512/1‑91 (MQ=255)
                                                                                                                 gAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTgg                                                                                         <  4:160512/91‑1 (MQ=255)
                                                                                                                  aGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCg                              <  2:48246/149‑1 (MQ=255)
                                                                                                                  aGCAGTACTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCg                              >  5:140154/1‑149 (MQ=255)
                                                                                                                         cTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGcgccgc                       >  3:115508/1‑149 (MQ=255)
                                                                                                                                 gCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCACGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACt               <  7:63840/149‑1 (MQ=255)
                                                                                                                                         atgttgttgtCAGCCTACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAg       >  3:231247/2‑149 (MQ=255)
                                                                                                                                           gttgttgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAgg      <  7:217076/148‑1 (MQ=255)
                                                                                                                                              gttgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTCAt  >  4:102407/1‑149 (MQ=255)
                                                                                                                                                tgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTc    >  7:217359/1‑145 (MQ=255)
                                                                                                                                                tgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTc    <  8:217359/145‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGCCATCTGCAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTCAT  >  NC_002947/4991087‑4991377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑