Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,991,239 G→T 8.6% intergenic (‑108/+121) PP_4399 ← / ← bkdR glutamine synthetase/Bkd operon transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,991,2390GT 8.6% 140.6 / 4.4 47intergenic (‑108/+121)PP_4399/bkdRglutamine synthetase/Bkd operon transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (19/23);  new base T (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

CAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTCAT  >  NC_002947/4991096‑4991377
                                                                                                                                               |                                                                                                                                          
cAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGacaac                                                                                                                                       <  2:219316/149‑1 (MQ=255)
cAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGac                                                                                                                                          <  4:86666/146‑1 (MQ=255)
  gacgacGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTCAGCCTacaacaa                                                                                                                                     >  6:60890/1‑149 (MQ=255)
     gacgGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTCAGCCTacaacaacaa                                                                                                                                  <  4:65080/149‑2 (MQ=255)
     gacgGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTCAGCCTacaacaacaa                                                                                                                                  <  5:60890/149‑2 (MQ=255)
      acgGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATa                                                                                                                                 <  8:103051/149‑1 (MQ=255)
         gCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGc                                                                                                                              <  3:248980/149‑1 (MQ=255)
            ttCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGc                                                                                                                           >  4:50525/1‑149 (MQ=255)
                           ggcggTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCAGGTGTTCAAAatat                                                                                                            <  5:80793/149‑1 (MQ=255)
                              ggTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATc                                                                                                           >  4:254724/1‑147 (MQ=255)
                                      ccTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTa                                                                                                 <  3:50525/149‑1 (MQ=255)
                                                   aaCGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCtgtg                                                                                    <  7:94973/149‑1 (MQ=255)
                                                    aCGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACAAAGGCTGCGGGTGTTCAAATTATCAagca                                                                                                      <  1:202253/130‑1 (MQ=255)
                                                    aCGCCGTTGAGGACGGGGGTGACGCATTCGATTTCCTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGATCAAAATATCAagca                                                                                                      >  2:202253/1‑130 (MQ=255)
                                                     cGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGcc                                                                                  <  7:314156/149‑1 (MQ=255)
                                                     cGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGcc                                                                                  >  8:314156/1‑149 (MQ=255)
                                                            gAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCctct                                                                           >  1:1844/1‑149 (MQ=255)
                                                               gTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         <  6:239513/100‑1 (MQ=255)
                                                               gTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         >  5:239513/1‑100 (MQ=255)
                                                                  gggggTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCgg                                                                     <  3:274262/149‑1 (MQ=255)
                                                                  gggggTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCgg                                                                     <  8:275788/149‑1 (MQ=255)
                                                                        gACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         >  3:255642/1‑91 (MQ=255)
                                                                        gACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCgg                                                                                                                         <  4:255642/91‑1 (MQ=255)
                                                                        gACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAAc                                                               >  1:48246/1‑149 (MQ=255)
                                                                          cGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAAccc                                                             >  1:151730/1‑149 (MQ=255)
                                                                           gCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCcg                                                            >  5:31685/1‑149 (MQ=255)
                                                                              ttCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCgcgc                                                         >  7:24842/1‑149 (MQ=255)
                                                                                 gATTTCGTCGATGCCCTGGATCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTAACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTTCACCcgcgaaga                                                      <  4:74745/149‑5 (MQ=255)
                                                                                          gATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGAt                                             <  2:1844/149‑1 (MQ=255)
                                                                                          gATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGAt                                             <  2:107499/149‑1 (MQ=255)
                                                                                           aTGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATg                                            >  5:208804/1‑149 (MQ=255)
                                                                                           aTGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATg                                            <  3:254724/149‑1 (MQ=255)
                                                                                           aTGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATg                                            <  6:31685/149‑1 (MQ=255)
                                                                                               ccTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGt                                                 >  2:90427/1‑140 (MQ=255)
                                                                                               ccTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGt                                                 <  1:90427/140‑1 (MQ=255)
                                                                                               ccTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGAt                                        >  8:63840/1‑149 (MQ=255)
                                                                                                        gAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTgg                                                                                         >  3:160512/1‑91 (MQ=255)
                                                                                                        gAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTgg                                                                                         <  4:160512/91‑1 (MQ=255)
                                                                                                         aGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCg                              <  2:48246/149‑1 (MQ=255)
                                                                                                         aGCAGTACTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCg                              >  5:140154/1‑149 (MQ=255)
                                                                                                                cTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGcgccgc                       >  3:115508/1‑149 (MQ=255)
                                                                                                                        gCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCACGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACt               <  7:63840/149‑1 (MQ=255)
                                                                                                                                atgttgttgtCAGCCTACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAg       >  3:231247/2‑149 (MQ=255)
                                                                                                                                  gttgttgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAgg      <  7:217076/148‑1 (MQ=255)
                                                                                                                                     gttgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTCAt  >  4:102407/1‑149 (MQ=255)
                                                                                                                                       tgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTc    >  7:217359/1‑145 (MQ=255)
                                                                                                                                       tgtAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTc    <  8:217359/145‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                          
CAGACGACGGCCTTCGAGGAAGCCTTCGGCGGTCATCACCTTGCCGCGCGGAACGCCGTTGAGGTCGGGGGTGACGCATTCGATTTCGTCGATGCCCTGGAGCTGAGCAGTGCTCATGACGCTTGTCCTTGTTGTTGTAGGCTGACAACAACATAGGCTGCGGGTGTTCAAAATATCAAGCAGCCTATTGGGTGGCTGTGCCGGCCTCTTCGCGGGTGAACCCGCGCCTACAGGTGGATGCGATCCTTGTGCCGGCGCCGCCCCCTACTCCCGCAAGGTCAT  >  NC_002947/4991096‑4991377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑