Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,044,759 C→G 7.4% intergenic (+65/+203) PP_4448 → / ← PP_5681 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,044,7590CG 7.4% 166.9 / 3.8 54intergenic (+65/+203)PP_4448/PP_5681hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base G (2/2);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01

GCCGACATTCCATACTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTCACATGCGATGTTCATCAAATCTCTC  >  NC_002947/5044611‑5044907
                                                                                                                                                    |                                                                                                                                                    
gCCGACATTCCATACTGTGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGAGcc                                                                                                                                                      >  4:235836/1‑149 (MQ=255)
       ttCCATACTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGt                                                                                                                                               <  1:12354/149‑1 (MQ=255)
         ccATACTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGtt                                                                                                                                              <  4:177571/148‑1 (MQ=255)
          cATACTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCGGCGCCTTCCTGAAGTTAGGTGCGGGGCCGttgg                                                                                                                                            <  6:353701/149‑3 (MQ=37)
            tACTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAgc                                                                                                                                          >  8:107550/1‑149 (MQ=255)
              cTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAgcgc                                                                                                                                        >  7:250722/1‑149 (MQ=255)
                    aTTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTc                                                                                                                                      >  5:297060/1‑145 (MQ=255)
                       gtgGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATc                                                                                                                                <  4:265205/148‑1 (MQ=255)
                           aaggaagAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGACGCCGGGCCGTTCAGCGCTCAAGATcagaag                                                                                                                           >  7:102166/1‑149 (MQ=255)
                              gaagaaTGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATcagaagcca                                                                                                                        <  8:250722/149‑1 (MQ=255)
                                   aTGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCGGCGCCTTCCTGAAGTTAGGTGCGGGGCCGttg                                                                                                                                             <  7:27117/123‑2 (MQ=25)
                                   aTGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCGGCGCCTTCCTGAAGTTAGGTGCGGGGCCGttg                                                                                                                                             >  8:27117/1‑122 (MQ=25)
                                    tGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCACACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCa                                                                                                                  <  8:130800/149‑1 (MQ=255)
                                          gATCTTAGCTTTGATCAGATCACGACTTAGTGGATTAAATAAGCACTTCTGCTCATCTGATAAACTAATCGCACCAACGGAACCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCAtttttt                                                                                                            <  5:353662/149‑1 (MQ=255)
                                            tCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTg                                                                                                           <  7:126867/148‑1 (MQ=255)
                                                 gCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATcagaagccagaa                                                                                                                     >  8:145913/1‑133 (MQ=255)
                                                 gCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATcagaagccagaa                                                                                                                     <  7:145913/133‑1 (MQ=255)
                                                  cTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGtt                                                                                                                                              <  6:5604/107‑1 (MQ=255)
                                                  cTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGtt                                                                                                                                              >  5:5604/1‑107 (MQ=255)
                                                    ttGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATcagaagccag                                                                                                                       >  1:206109/1‑128 (MQ=255)
                                                    ttGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATcagaagccag                                                                                                                       <  2:206109/128‑1 (MQ=255)
                                                     tGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGACGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAg                                                                                                   <  8:102166/147‑1 (MQ=255)
                                                         cagatcaCGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTc                                                                                             >  3:2163/1‑149 (MQ=255)
                                                            atcaCGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCg                                                                                           >  4:92533/1‑148 (MQ=255)
                                                            atcaCGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCg                                                                                           >  6:207868/1‑148 (MQ=255)
                                                                      aGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGc                                                                                  <  5:207868/147‑1 (MQ=255)
                                                                          gCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGc                                                                            >  1:345851/1‑149 (MQ=255)
                                                                                 tAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGt                                                                                          >  2:347739/1‑128 (MQ=255)
                                                                                 tAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGt                                                                                          <  1:347739/128‑1 (MQ=255)
                                                                                     cACTGCTGCTGATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTGAGGAAGGCGCCGGGCCGTTCAGCGCTCAATATCATAAGGCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGTGCTCCTCCGTcgc                                                                 <  6:297060/149‑1 (MQ=255)
                                                                                     cACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTcgc                                                                 <  3:92533/149‑1 (MQ=255)
                                                                                              tCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTAAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGATTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCt                                                        >  1:85825/1‑149 (MQ=255)
                                                                                                aTCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGa                                                      <  2:345851/149‑1 (MQ=255)
                                                                                                     agaAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCa                                                 <  4:2163/149‑1 (MQ=255)
                                                                                                      gaAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTc                                                           >  6:367368/1‑138 (MQ=255)
                                                                                                      gaAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTc                                                           <  5:367368/138‑1 (MQ=255)
                                                                                                      gaAACGAAGCCCCCCTACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGAATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCaa                                                <  4:38004/149‑1 (MQ=255)
                                                                                                       aaaCGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCGGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGCGCTCCTCCGTCGCGCATTCCCTGATTCCAAg                                               >  1:252878/1‑149 (MQ=255)
                                                                                                         aCGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGct                                             >  5:81803/1‑149 (MQ=255)
                                                                                                                 cACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGc                                                                            >  6:53209/1‑110 (MQ=255)
                                                                                                                 cACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGc                                                                            <  5:53209/110‑1 (MQ=255)
                                                                                                                    cAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGGTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGCTACCAAGCTCTAGATCCAGc                                  >  6:328295/1‑149 (MQ=255)
                                                                                                                        ggCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCcttc                              >  3:342748/1‑149 (MQ=255)
                                                                                                                           cccGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCttcttc                           <  3:235836/149‑1 (MQ=255)
                                                                                                                            ccGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTc                                                           <  8:190994/116‑1 (MQ=255)
                                                                                                                            ccGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTc                                                           >  7:190994/1‑116 (MQ=255)
                                                                                                                              gCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTcaca                        <  7:107550/149‑1 (MQ=255)
                                                                                                                                  cTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCTAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCagag                                                                             <  2:343106/92‑1 (MQ=255)
                                                                                                                                  cTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCTAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCagag                                                                             >  1:343106/1‑92 (MQ=255)
                                                                                                                                  cTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGcc                                 <  1:34301/136‑1 (MQ=255)
                                                                                                                                  cTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGcc                                 >  2:34301/1‑136 (MQ=255)
                                                                                                                                  cTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAACCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGACAGAGCTCCTCCGTCTCGCATACCCTGATACCAAGCTCTAGATACAGACTTCTTCAAATGc                     >  8:322543/1‑148 (MQ=255)
                                                                                                                                       ttCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTCACATGCGATGtt               >  6:210239/1‑149 (MQ=255)
                                                                                                                                            gAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTCACATGCGATGTtcatc           >  5:298776/1‑148 (MQ=255)
                                                                                                                                            gAAGGCGCCGGGCAGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTCACATGCGATGTtcatc           >  4:85288/1‑148 (MQ=255)
                                                                                                                                                    cGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTCACATGCGATGTTCATCAAAtctctc  <  6:298776/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGACATTCCATACTGGGAATTGTGGAAGGAAGAATGGAAAGATCTTAGCTTTGATCAGATCACGACTGAGTGGCTTAAATAAGCACTGCTGCTCATCTGAGAAACGAAGCGCACCAACGGCCCCGCACCTAACTTCAGGAAGGCGCCGGGCCGTTCAGCGCTCAAGATCAGAAGCCAGAAGCATTTTTTGGAAGGTAGGGCTTCCGTAATGTTGCCAGAGCTCCTCCGTCGCGCATTCCCTGATACCAAGCTCTAGATCCAGCCTTCTTCACATGCGATGTTCATCAAATCTCTC  >  NC_002947/5044611‑5044907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑