| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,084,580 | C→T | 13.7% | intergenic (‑315/+70) | alaS ← / ← astE | alanine‑‑tRNA ligase/succinylglutamate desuccinylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,084,580 | 0 | C | T | 13.7% | 68.4 / 2.9 | 30 | intergenic (‑315/+70) | alaS/astE | alanine‑‑tRNA ligase/succinylglutamate desuccinylase |
| Reads supporting (aligned to +/- strand): ref base C (15/10); new base T (2/2); total (18/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01 | |||||||||||
TGTGTTTCGCCACTGGCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCTTGGGGTTGGGG > NC_002947/5084443‑5084725 | tgtgTTTCGCCACTGGCAAGCCGATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACAtt > 7:280218/1‑149 (MQ=255) tggttCGCCACTGGCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACAtt < 8:292198/144‑1 (MQ=255) gCCACTTGAAAGCAAATTACCGCTCAAGGGAGGTGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCtt < 6:174667/149‑1 (MQ=255) gCCACTGGCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTAGCGGGTAAACCCGCTCCTACGGGCAC‑GGCCCCACATTGAGACCtt > 1:340284/1‑149 (MQ=255) gCCACTGGCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCATTTTACCGGGTAAACCCCCTCCGACGGGCCA‑GGCCCCACAATGAGACCtt > 5:231026/1‑149 (MQ=255) ccACTGGCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTg > 6:127533/1‑149 (MQ=255) ggCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGcaca > 5:12352/1‑148 (MQ=255) ccAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCGCCACATTGAGACCTTGCACATATCTCg > 7:179355/1‑149 (MQ=255) aCCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAg > 3:240680/1‑146 (MQ=255) aCCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAg < 4:240680/146‑1 (MQ=255) ccGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCCGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGCGCCCTTGCCCATATCTCGTATGAGc > 2:332528/1‑149 (MQ=255) gCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCgg < 8:280218/149‑1 (MQ=255) aaGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACat > 5:361550/1‑130 (MQ=255) aaGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACat < 6:361550/130‑1 (MQ=255) ggagggGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACAATGAGACCTTGCACATATCTCGTAGGAGCGGGGTCAcc > 3:304802/1‑149 (MQ=255) agggGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCGCCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCa < 8:179355/149‑1 (MQ=255) aTGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAg < 8:156362/149‑1 (MQ=255) aGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTgag > 4:192065/1‑85 (MQ=255) aGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTgag < 3:192065/85‑1 (MQ=255) gatCCGAAACTGTGTCGCCTGTTTCGCGTGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCa > 1:202440/1‑149 (MQ=255) gatCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAT‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCa > 2:54858/1‑149 (MQ=255) gggTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATg > 3:60832/1‑133 (MQ=255) gggTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATg < 4:60832/133‑1 (MQ=255) gTAAACCCGCTCCTACGGGCAT‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTc > 1:231057/1‑149 (MQ=255) gTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTc > 7:242245/1‑149 (MQ=255) aCCCGCTCCTACGGGCAT‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGa < 2:231057/149‑1 (MQ=255) cccGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGAc > 2:33848/1‑149 (MQ=255) ccGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGAcc > 2:350344/1‑149 (MQ=255) cccatccGGCCAT‑GGCACCACTTTGACACCTTCCAAATCTCTCTTAGGCGCGGGGTCACCCACCAAACTGGCTACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCttg < 1:54858/143‑1 (MQ=255) cTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCttg < 1:350344/149‑1 (MQ=255) ctcgggcacgggcaCAATATTCCCACCTGCCACATCTCTCGTTGGTGGGGGTCCACCAACCAAACTGGCTACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCttgggg < 5:80131/147‑1 (MQ=255) gcac‑ggcacCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCtt < 4:54196/139‑1 (MQ=255) gcac‑ggcacCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCtt > 3:54196/1‑139 (MQ=255) ac‑ggcacCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCttggggttgggg > 8:170530/1‑147 (MQ=255) | TGTGTTTCGCCACTGGCAAGCCAATTACCGCTCAAGGGAGGGGAAAATGGAACAGGCGGTGAATTAAAGGGTTTTCATCAAGGATAGGATCCGAAACTGTGTCGCCTGTTTCGCGGGTAAACCCGCTCCTACGGGCAC‑GGCACCACATTGAGACCTTGCACATATCTCGTAGGAGCGGGGTCACCCACGAAACAGGCGACACCGCTCTCACCCCAGGCGCTGCCCCGAATCCGGCACGATCAGAATGCCGGCGCGCAAGCCGTTCTTGACCTTGGGGTTGGGG > NC_002947/5084443‑5084725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |